Lipoamide dehydrogenase and diaphorase catalyzed conversion of some NO donors to NO and reduction of NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO).

One of the key functions of nitric oxide (NO) in human is to dilate blood vessels. We tested glycerol trinitrate (GTN) and other well-known NO donors together with those bearing a >C=N-OH group for possible conversion to NO (or nitrites, respectively) by diaphorase (DP) and lipoamide dehydrogenase (LAD). Both, DP and LAD were unable to convert formamidoxime (FAM), acetone oxime (AC), acetohydroxamic acid (AHA) and Nomega-hydroxy-L-arginine (L-NOHA).

Labelling of the anti-III-neurotubulin monoclonal antibody by 99mTc and its binding to responsible antigen.

Background: beta-III-neurotubulin is a characteristic degradation protein of cellular cytoskeleton of nervous tissues, which originates with disorders that lead to the loss of peripheral neurons or neurons of the central nervous system. For the diagnostics of neuropathic and neurodegenerative processes, beta-III-neurotubulin is therefore the goal structure, and radioactive labelled antibody TU-20 (99m)Tc could theoretically enable diagnostic application in vivo.

Material And Methods: In our case, were selected three ways of labelling monoclonal antibody IgG1-TU-20, which shows a high affinity towards beta-III-neurotubulin with (99m)Tc.