Genotoxic potential of two herbicides and their active ingredients assessed with comet assay on a fish cell line, epithelioma papillosum cyprini (EPC).

The aim of this study was to optimize the epithelioma papillosum cyprini (EPC) cell line handling procedure for the comet assay to investigate the genotoxic potential of widely used pesticides. The effects of various media and handling of the EPC cell line were examined. Results indicated that avoiding trypsin to detach cells led to lower level of DNA damage in the negative control.

Comet assay on tetraploid yeast cells.

Tetraploid yeast cells (Saccharomyces cerevisiae) were used in the comet assay with the intention of developing a new, fast and easy assay for detecting environmental genotoxic agents without using higher organisms. Two DNA-damaging chemicals, H(2)O(2) and acrylamide, together with wastewater from three municipal treatment plants were tested for their effect on the yeast-cell DNA. The main problem with using yeast in the comet assay is the necessity to degrade the cell wall.

Monitoring DNA damage in indigenous blue mussels (Mytilus edulis) sampled from coastal sites in Denmark.

Damage to DNA detected by use of the single-cell gel electrophoresis (comet) assay was monitored in blue mussels, Mytilus edulis, sampled from coastal waters in Denmark. Mussels from five locations in Køge Bay, an area receiving wastewater from many industries and municipalities, were collected five times during 1999 and six times in 2001. In 1999, both gill cells and haemolymph cells were examined, and sediments were sampled on three dates from the same five locations.

Comet assay on gill cells and hemocytes from the blue mussel Mytilus edulis.

Gill cells and hemocytes from the blue mussel Mytilus edulis were examined for DNA damage using the comet assay after laboratory exposure in vitro and in vivo to methyl methansulfonate (MMS). Hydrogen peroxide and UV radiation were used as positive control. Comet assay was also carried out on hemocytes from blue mussels sampled at polluted and unpolluted coastal areas.