Publications by authors named "Kiyoko Higashiyama"
Parvoviruses have evolved to possess a linear single-stranded DNA (ssDNA) genome ranging from 4 to 6.3 kb. Adeno-associated virus (AAV), a member of the Parvoviridae family, contains approximately 5 kb of linear ssDNA within its capsid.
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- Adeno-associated virus (AAV) vectors are important for gene therapy but are produced with various impurities, making purification essential.
- The study highlights the use of equilibrium density gradient analytical ultracentrifugation (DGE-AUC) to accurately quantify full and empty AAV particles, and to identify other components.
- While DGE-AUC is effective for assessing particle density and purity, it has limitations in detecting minor components with low absorption or similar densities to major AAV components, thus complementing other methods like sedimentation velocity AUC (SV-AUC) and band sedimentation AUC (BS-AUC).
Recombinant adeno-associated virus (rAAV) is a prominent viral vector currently available for human gene therapy. The diameter of the rAAV capsid is ∼25 nm, and a positive or negative single-stranded DNA is packaged within the vector capsid. In this report, we describe a concise method to examine the extracted rAAV genome using an automated electrophoresis system.
View Article and Find Full Text PDFRecombinant adeno-associated virus (rAAV) is a viral vector commonly used in gene therapy. Residual host cell DNA is an impurity that has been associated with the risk of infection and oncogenicity. Thus, it needs to be monitored for quality control.
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