2,3-diphosphoglycerate and oxygen supply of tissues in cardiosurgical diabetics.

The Main Aim Of The Study: The oxygen supply of tissues was studied under haemodilution in cardiosurgical diabetic and non-diabetic patients.

Methods: There were 30 cardiosurgery patients examined, 9 were patients with diabetes mellitus.and 21 were non-diabetic patients.

Systemic blood pressure response to the inhibition of two hyperpolarizing pathways: a comparison to NO-synthase inhibition.

The impact on blood pressure of two vasodilating mechanisms, underlied by vascular smooth muscle hyperpolarization, was studied and compared to that induced by nitric oxide NO mechanism. Systemic blood pressure, after inhibitory intervention in arachidonic acid metabolism cytochrome P-450 inhibition by miconazole 0.5 mg/100 g b.

The assessment of erythrocyte deformability by filtration rate.

The aim of the study was to monitor the erythrocyte deformability as one of important factors securing the appropriate tissues perfusion in healthy subjects and diabetic patients with insulin dependent diabetes mellitus. Erythrocyte deformability was determined by the method of filtration and centrifugation, and the erythrocyte filtrability was calculated as a percentage of filtered erythrocytes out of the number of erythrocytes counted before centrifugation. Diluted blood suspensions were filtered by centrifugation through membrane filters with pores of 5 microm in diameter.

The effect of selected membrane active substances on erythrocyte deformability.

Aminoguanidine improved the erythrocyte filterability by 4%, pyridoxyliden-aminoguanidine by 11% and pyridoxal by 13% in healthy subjects. In diabetic patients the aminoguanidine effect on erythrocyte filterability was improved by 7%, PAG effect by 9% and pyridoxal effect by 15% in comparison to the control group. The other investigated haematological variables in both groups were within the range of the physiological standard.

[Oxygen transport values in patients with surgery performed under extracorporeal blood circulation].

The aim of the work was to study the O2-transport changes to tissues in cardio-surgical patients suffering from CAD and operated during extra-corporeal blood circulation (ECC). The changes of selected haematologic variables, 2,3-diphosphoglycerate (2,3-DPG) and ATP concentration, acid-base balance parameters with p50 calculation were measured in the venous blood samples taken before the operation, during the operation and on the 1st, 2nd, 3d, 5th, 7th and 10th day after the operation. From the obtained results follows, that extreme haemodilution causes significant decrease of the haematocrit value (Htc) by 35%, the value of haemoglobin (Hb) by 37% and the count of erythrocytes (Er) by 37% from the initial values.

Early changes of protein synthesis in myocardium and coronary arteries induced by NO synthase inhibition.

The question was addressed whether short-term (4 hour) NO deficiency, inducing an increase in blood pressure in anaesthetized dogs, does influence proteosynthesis in the myocardium and coronary arteries. A potentially positive answer was to be followed by the study of the supporting role of ornithine decarboxylase for the polyamines pathway. N(G)-nitro-L-arginine-methyl ester (L-NAME) (50 mg/kg per hour) was administered i.

NO concentration in the periendothelial area of the femoral artery of the dog measured in vivo.

.NO concentration was measured in the periendothelial area of the femoral artery by Malinski's porphyrinic .NO sensor in seven anaesthetized dogs.

Early changes in protein synthesis in epicardial coronary artery of pressure-overloaded heart.

In anesthetized dogs, a 4-h, approximately 30% increase in blood pressure induced by constriction of the abdominal aorta brought about an increase in the total RNA content in the left anterior descending coronary artery (LADCA) and the left ventricular (LV) myocardium (9.05 +/- 1.72 and 11.

Nitric oxide in the periendothelial area of femoral vein of the dog assessed in vivo by a porphyrinic sensor.

Nitric oxide concentration in the periendothelial are of the femoral vein in anaesthetized dogs was measured directly with a catheter- protected porphyrinic sensor. A 2- to 4-fold increase occurred in the basal NO concentration of 90 +/- 12 nM after acetylcholine injection (1-1.5 micrograms/kg).

Biomechanical signals in the coronary artery triggering the metabolic processes during cardiac overload.

Peculiarities in structure and deformability of epicardial conduit coronary arteries are described. The thin wall of animal coronary artery contrasts the human coronary artery in which the remarkable wall thickness is due namely by the intima thickness. Deformation in length and diameter of conduit coronary arteries, due to the left and right ventricle volume increase, has been defined in non-beating canine heart.

Protein metabolism in coronary wall affected by cardiac load.

The relation between biomechanics of conduit coronary artery and metabolic processes in its wall were studied in cardiac pressure overload, in anaesthetized dogs. Two sorts of forces and/or coronary deformations were followed: (1) radial and length deformation of ramus interventricularis anterior (RIA) due to ventricle volume changes, and (2) shear stress in ramus circumflexus (RC) ensuing the blood flow changes. Increase in aortic pressure (33.

Blood pressure and vasomotor responses to sympathetic stimuli in patients with chronic myocardial infarction.

The responses of resistance and capacitance vessels of skin and muscle, and of blood pressure to reflex influences mediated by the sympathetic system were investigated in a group of 63 male patients in a chronic phase of myocardial infarction. In the group of patients with MI, as compared to the controls: (1) the reactive increase of systolic BP and heart rate was significantly smaller but longer lasting after cessation of the stimuli; (2) the vasoconstrictory reactions in skin were significantly prolonged in resistance as well as in capacitance segments of vascular bed; and (3) the resting blood flow in muscle was significantly lower and the vasodilatation during an emotional reaction was less pronounced and shorter. Possible underlying mechanisms of this vasomotor pattern are discussed.