Publications by authors named "Kittiya Intaruck"
Article Synopsis
- Fruit bats can carry dangerous viruses like Nipah and Hendra, which can affect humans and animals.
- Scientists found a new type of virus called MRV from fruit bats in Indonesia, showing it's the first time this virus has been detected in Southeast Asia.
- The research revealed that this virus could make laboratory mice very sick, indicating it might be a serious health risk for both animals and people.
Article Synopsis
- - Rabies virus (RABV) is a deadly virus that causes rabies, categorized into fixed laboratory strains and street strains found in the wild, each with different traits.
- - The Toyohashi strain is a street strain from Japan that was derived from a rabid dog bite and has been used to create a reverse genetics system for research.
- - Researchers developed a recombinant Toyohashi strain that allows tracking of viral proteins in living cells, making it a valuable resource for studying how rabies street strains replicate at the molecular level.
Article Synopsis
- - The study investigates the effectiveness of monotherapy with oral antiviral ensitrelvir and the anti-inflammatory corticosteroid methylprednisolone, as well as their combination, in treating hamsters infected with SARS-CoV-2, focusing on timing and therapeutic outcomes.
- - Results showed that combining ensitrelvir and methylprednisolone significantly improved respiratory health and decreased pneumonia risk, even when treatment started two days post-infection, by reducing lung damage and inflammation.
- - The findings underscore the potential benefits of using a combination therapy of antiviral and corticosteroid drugs for better lung pathology and inflammatory response management in COVID-19 cases, including those caused by the delta and omicron variants.
The most conserved fusion loop (FL) domain present in the flavivirus envelope protein has been reported as a dominant epitope for cross-reactive antibodies to mosquito-borne flaviviruses (MBFVs). As a result, establishing accurate serodiagnosis for MBFV infections has been difficult as anti-FL antibodies are induced by both natural infection and following vaccination. In this study, we modified the most conserved FL domain to overcome this cross-reactivity.
View Article and Find Full Text PDFViral protein assembly and virion budding are tightly regulated to enable the proper formation of progeny virions. At this late stage in the virus life cycle, some enveloped viruses take advantage of the host endosomal sorting complex required for transport (ESCRT) machinery, which contributes to the physiological functions of membrane modulation and abscission. Bullet-shaped viral particles are unique morphological characteristics of rhabdoviruses; however, the involvement of host factors in rhabdovirus infection and, specifically, the molecular mechanisms underlying virion formation are not fully understood.
View Article and Find Full Text PDFNelson Bay orthoreovirus (NBV) is an emerging bat-borne virus and causes respiratory tract infections in humans sporadically. Over the last two decades, several strains genetically related to NBV were isolated from humans and various bat species, predominantly in Southeast Asia (SEA), suggesting a high prevalence of the NBV species in this region. In this study, an orthoreovirus (ORV) belonging to the NBV species was isolated from Indonesian fruit bats' feces, tentatively named Paguyaman orthoreovirus (PgORV).
View Article and Find Full Text PDFAir-liquid interphase culture confers SARS-CoV-2 susceptibility to A549 alveolar epithelial cells.
Biochem Biophys Res Commun
November 2021
Article Synopsis
- A549 lung cells are normally resistant to SARS-CoV-2 due to low levels of the entry receptor ACE2 when grown in standard submerged cultures.
- Adapting A549 cells to an air-liquid interface (ALI) culture causes them to develop a mucus layer and reduces a proliferation marker, making them more susceptible to SARS-CoV-2 infection.
- This increased susceptibility is linked to higher expression of ACE2 and TMPRSS2 in ALI culture, and treatment with Camostat, a TMPRSS2 inhibitor, effectively reduced the infection rate in these modified cells.
Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) possesses a discriminative polybasic cleavage motif in its spike protein that is recognized by the host furin protease. Proteolytic cleavage activates the spike protein, thereby affecting both the cellular entry pathway and cell tropism of SARS-CoV-2. Here, we investigated the impact of the furin cleavage site on viral growth and pathogenesis using a hamster animal model infected with SARS-CoV-2 variants bearing mutations at the furin cleavage site (S gene mutants).
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