[The study of experimental and natural rotavirus infection in mice using a heterologous enzyme immunoassay test-system].

Enzyme immunoassay test systems constructed on the basis of heterologous calf and monkey rotaviruses were shown to be applicable for diagnosis and study of the pathogenesis of experimental and natural rotavirus infection of mice.

[Development of test systems of immunoenzyme analysis for the rapid diagnosis of rotavirus infection].

A test system of enzyme immunoassay (EIA) consisting of simian rotavirus SA-11 and rabbit antiserum has been developed for the detection of rotavirus antigen. Direct EIA was used for tests on stool specimens from 289 children varying in ages from 10 days to 12 years suffering from acute enteric infections and 56 normal children. The antigen was detected in 22.

[Suppression of rotavirus SA-11 reproduction by protease inhibitors in cell culture].

The effect of proteases inhibitors, epsilon-amino-caproic acid and gordox, on reproduction of rotavirus SA-11 in MA-104 cells was studied by enzyme immunoassay. These inhibitors were shown to exert an inhibiting effect on rotavirus reproduction.

[Scanning microscopy of hamster cells transformed by herpes simplex virus type 2].

Scanning microscopy was used to examine the features of morphology and attachment to a solid substrate of a transformed and tumor lines of hamster cells. These cell lines differed from normal hamster fibroblasts by changes in the mode of attachment and the degree of flattening on the solid substrate, relief of the cell surface and pattern of intercellular interactions. The observed morphological changes correlated with the degree of cell transformation.

[Mechanism of the cellular transformation of newborn hamsters inoculated with herpes simplex type 2 virus].

Combined virological, antigenic, electron microscopic, and molecular biologic study of the role of an oncovirus, herpes simplex type 2 virus (HSV-2), in the mechanisms of transformation of HSV-2-infected hamster cells was carried out. No expression of genetic information of the oncovirus could be detected. Cell transformation was shown to be associated with persistence and realization of HSV-2 genetic information in the transformed cells.

[Morphological, cytogenetic and proliferative characteristics of Syrian hamster HTC-2 and HTC-1 cells and of the HTCT tumor cell line transformed by herpes simplex type 2 virus].

Morphological, caryological, cytoproliferative, and isoenzyme studies of Syrian hamster cells HTC-2 and HTC-1 transformed by herpes simplex virus type 2 and a HTCT line of tumor cells were carried out. The hamster origin of these cell lines was established by chromosomal analysis and electrophoretic mobility of lactate dehydrogenase and glucose-6-phosphate-dehydrogenase isoenzymes. The transformed and tumor cell lines differ from normal cells by altered morphology, an aneuploid chromosome set and increased proliferative activity.

[Malignant transformation of newborn hamster cells by herpes simplex virus type 2].

The oncogenic potentials of HSV-2 inactivated by ultraviolet rays in newborn hamster cell culture were studied. Virus-induced morphological and malignant transformation of cells was accompanied by synthesis of virus-specific antigen, changes in morphology, formation of colonies in semi-liquid agar, and tumorigenicity of cells. The animals bearing tumors induced by transformed cells showed humoral and cellular immune responses to the virus-specific antigen.

[Cellular immune response detected in blast transformation test with lymphocytes from rabbits immunized with nucleocapsid of herpes simplex type 2 virus].

A preparation of herpes simplex virus type 2 (HSV-2) nucleocapsid (NC) was obtained after treatment of intracellular virus with detergents followed by centrifugation in linear sucrose density gradient. Electron microscopic analysis revealed polymorphism of HSV-2 NC population. Three main types of capsid structures were found: empty capsids, NC containing various amounts of nucleoid material, and NC with complete nucleoid.

[Etiological role of the herpes simplex virus in causing cervical cancer].

The results of the composite virological and immunological examinations of 46 patients with cervical carcinoma (CC) attest to the association of herpes simplex virus (HSV) with neoplastic processes. HSV strains were isolated from the tumour tissue as well as from the cervical canal secretions and blood of 4 patients. A higher level of virus-neutralizing and complement-fixing antibodies to HSV was found in sera from CC patients as compared with the control group of normal women.

[Modelling of viral-chemical carcinogenesis in chronic infection in mice caused by the herpes simplex type 2 virus].

The influence of chronic herpetic infection of white mice caused by herpes simplex virus type 2 (HSV-2) on the blastomogenic effect of a polycyclic hydro-carbon, 20-methylcholanthrene (20-MCA), was studied. Solid tumors developed in 20-MCA-treated mice on the side of the carcinogen administration within 81--89 days. The simultaneous administration of 70 intracerebral LD50 of HSV-2 subcutaneously into a hind leg pad and 0.

[Comparative study of the effectiveness of the purification and concentration of herpes simplex virus types 1 and 2].

The results of comparative studies on concentration and purification of herpes simplex virus type 1 and type 2 (HSV-1 and HSV-2) by ficol density gradient centrifugation are presented. A two-phase distribution of extracellular HSV was established in phycol density gradient centrifugation: in zones with density of 1.110-1.

[Comparative study of the oncornavirus A and D proteins of human J-96 cells by the methods of isoelectric focusing and polyacrylamide gel electrophoresis].

Three peaks of 14C-radioactivity with buoyant densities of 1.23--1.24, 1.

Radioimmunoassay of type D oncovirus from continuous J-96 cells.

Radioimmunoassay of J-96 virus and an extract of J-96 cells in the homologous and heterologous systems aimed at detecting antigenic determinants of p25 of Mason-Pfizer virus, as well as group-specific and interspecies antigenic determinants p30 of Rauscher leukaemia virus demonstrated that (1) J-96 virus contains a major internal protein immunologically identical with p25 protein of Mason-Pfizer virus based on the antigenic determinants detectable by the radioimmunoassay used; and (2) no interspecies antigenic determinants characteristic of the major internal protein of mammalian type C viruses were detectable in the J-96 virus or the J-96 cell extract.

[Protein metabolism in human embryo skin-muscle tissue cells infected with oncornavirus D from J-96 cells].

Structural polypeptides of oncornavirus D produced by J-96 cells and the effect of oncornavirus infection on protein metabolism of human embryo skin-muscle tissue cells (HESM) were studied. Electrophoresis showed the number of polypeptides detectable in a preparation of virus produced by infected HESM cells to depend on the number of centrifugations in sucrose gradient in the process of virus purification. A preparation of a singly purified virus was found to contain 13 polypeptide components: p115, p110, p100, p87, p78, p69, p57, p45, p36, p27, p15, p12, and p10.

[Effect of exogenous interferon on the course of latent oncornavirus infection of J-96 cells].

The effect of exogenous leukocyte interferon on the course of chronic oncornavirus infection of lymphoblastoid J-96 cells chronically producing type B virus was studied. By means of radio-isotope analysis and electron microscope examinations it was shown that upon long-term passage of the cells (18-34 passages) in the presence of interferon (10 units/ml) the process of virion formation in the cells was inhibited 2.0-4.

[Assessment of the sensitivity of immunoprecipitation for the serodiagnosis of influenzal-bacterial pneumonias].

A comparative examination of 226 paired sera from patients with pneumonia was carried out by CFT, HI, neuraminidase activity inhibition (NI) and double immunodiffusion. A correlation of the results of agar gel precipitation and the CFT and HI tests was observed. Convalescent sera contained antibody to influenza virus ribonucleoprotein frequently, less so to its neuraminidase or hemagglutinin.

[Analysis of influenza virus reproduction under conditions of chronic infection].

The results of comparative studies of influenza virus, original and produced in KM cells under conditions of chronic infection (KM WSN) are presented. The WSN KM virus was shown to band in the density range of 1.18 to 1.

[Comparative antigenic analysis of J-96 cell oncornavirus and Mason-Pfizer virus from a spontaneous monkey mammary tumor].

A comparative study of the antigenic structure of oncornavirus of J-96 cells and Mason-Pfizer virus (M-PVM) isolated from cells of M. rhesus spontaneous mammary tumour was carried out using immunodiffusion in agar, immune autoradiography and indirect immunofluorescence procedures. Immune rabbit serum to disrupted J-96 virus detected by agar immunodiffusion in preparations or purified virus three soluble antigens one of which was identical to group-specific M-PVM antigen.

[Further study of J-96 transplantable cell oncornavirus].

The symplast-forming capacity of oncornavirus of continuous J-96 cells was studied by KS-test. Syncytia were formed upon combined cultivation of KS cells with J-96 cells and cells of human embryo skin-muscle tissue infected with J-96 virus as well as upon direct inoculation of KS cells with oncornavirus J-96. Formation of syncytia of KS cells correlated with the amount of J-96 cell oncornavirus.