[The cytopathic and cytogenetic sequelae of chronic inhalational exposure to formaldehyde on female germ cells and bone marrow cells in rats].

A study was made of cytopathological and cytogenetic effects of formaldehyde chronic inhalation, in doses 0.5 and 1.5 mg/m3, on the female rat's germ and marrow cells.

Urethane-induced lung adenomas in the first-generation progeny of irradiated male mice.

A carcinogenic effect of urethane on the first-generation progeny of irradiated male mice was investigated. Seventy SHR male mice were exposed to a single total-body X-ray irradiation at a dose of 4.2 Gy.

[Structural and functional indices of disturbances of oocyte maturation in androgenized rats after induction of ovulation with pregnant mare's serum and luliberin].

The ultrastructure of oocytes from large follicles and the meiotic maturation rate of preovulatory oocytes of intact and androgenized rats were studied. The oocytes of defeminized animals were characterized by the lack of organelles and by changes of their structure. According to the rate of meiosis they were divided into two groups.

[Interrelation of the hormonal indices and the cytologic characteristics of the germ cells].

The analysis of the maturation of follicular oocytes as well as of the state of gametes after induction of ovulation with PMSG or with GnRH in intact and androgenized rats revealed the dependence of the heterogeneity extent (in morphologic and chromosomal damages) of population of germ cells maturing in vivo and in vitro upon the character of gonadotropin regulation disturbances of folliculogenesis. Stable disorder of reproductive function (androgenization) is followed by a significant increase in number of abnormal gametes under its hormonal correction.

[Human extracorporeal fertilization with subsequent successful embryo implantation and the birth of a child].

With the aim to use a previously elaborated method of extracorporeal fertilization of human ova for treating sterility, resulted from obstruction or absence of the uterine tubes, ova were obtained at laparoscopy after a previous hormonal stimulation of women. From 119 women 159 ova were obtained. Fertilization of the ova was performed in the medium Ham F-10 previously capacitated with the husband's spermatozoa.

[Changes in the nature of the estrous cycle and gametogenesis during superovulation stimulation in rats at different stages of the cycle].

Estrous cycle, ovulation rate and gamete quality were studied in rats with superovulation induced on different days of the cycle. It was shown that the rat ovaries in estrous were most sensitive to the action of gonadotropins. Heterogeneity in the ovulation rate was noted in animals at the same phase of the cycle.

[Effect of an estrogen-progestin preparation alone and in combination with amizil on the structure and electrophoretic parameters of oocytes and early rat embryos].

An increasing contraceptive effect at a combined administration of steroid drugs with neurotropic substances--M-cholinolytic benactyzine, makes the problem on the character and mechanism of their action. In the experiments performed in rats, under effect of ethinyl estradiol and norethisteron acetate, and especially at their combination with benactyzine, the first signs of overmaturation are revealed in oocytes situating in the follicular cavity. Therefore, oocytes revealed in the rat fallopian tubes leave behind the development of the control oocytes.

[Ultrastructure of rat oocytes during the induction of superovulation].

The ultrastructure of ovocytes, fixed no later than 4-5 hours after the beginning of ovulation, the number of cells with the signs of organelle alterations is increased. This is presumably connected with a change in conditions of hormonal regulation of late stages of gametogenesis.

[The state of gametes and the type of maturation of follicular oocytes in rats neonatally androgenized by LH-RH-induced ovulation].

The character of follicular oocyte maturation and the state of gametes in androgenized rats was studied after LH-RH stimulation. The induction of ovulation with LH-RH has been shown to increase the number of rats with nonovulatory follicules and reduce the number of oocytes maturing prior to the onset of metaphase II. After the induction of ovulation the rate of chromosomal aberrations became higher than in intact animals.

[Effect of LH releasing hormone on the state of gametes and the maturation of follicular oocytes in vitro].

Ovulation, gametogenesis and maturation of rat follicular oocytes were examined in vitro under the effect of single and repeated injections of LH-RF. It was shown that LH-RF injection led to incomplete ovulation and to alteration of the heterogeneity of the gametes as regards the degree of maturation and the number of the degenerating forms. At the same time it did not produce any substantial effect on the rate of chromosomal abnormalities.

[Human follicular oocytes (cytologic and physiologic characteristics)].

The morphology and the rate of degenerative changes were studied in the oocytes recovered from the antral follicles of the operated women. The rate of meiosis resumption, chromosome aberrations and electrophysiological properties were studied in the cultivated oocytes. These indices were compared in women of different age and pathology.

[Comparison of the frequency of chromosomal disorders in populations of in vitro-matured and ovulating rat oocytes].

The rat oocytes extracted from the rat ovaries and cultivated for 42-46 hours were compared with ovulated oocytes by the chromosomal aberration rate. The chromosomal aberration rate in the population of "follicular" oocytes was 8.2% on the average whereas in ovulated oocytes, it did not exceed 1.