Features of the interaction of 5-[4'-(6″-aminopurin-2″-yl)phenyl]-10,15,20-tri(N-methylpyridin-3'-yl)-porphyrin with nucleic acids.

Directed synthesis of novel water-soluble asymmetric porphyrins containing in a molecule three cationic fragments and residues of adenine (PorAD) was performed, using metal-complex catalysis method. The interaction of the synthesized porphyrin with the oligonucleotides poly[d(AT)2] and poly[d(GC)2] and double-stranded deoxyribonucleic acid of the calf thymus (ctDNA) was studied by means of spectral and hydrodynamic methods. It was established that PorAD intercalated not only into GC-enriched regions, but also into AT regions.

Self-organizing human heart assembloids with autologous and developmentally relevant cardiac neural crest-derived tissues.

Unlabelled: Neural crest cells (NCCs) are a multipotent embryonic cell population of ectodermal origin that extensively migrate during early development and contribute to the formation of multiple tissues. Cardiac NCCs play a critical role in heart development by orchestrating outflow tract septation, valve formation, aortic arch artery patterning, parasympathetic innervation, and maturation of the cardiac conduction system. Abnormal migration, proliferation, or differentiation of cardiac NCCs can lead to severe congenital cardiovascular malformations.

Human heart assembloids with autologous tissue-resident macrophages recreate physiological immuno-cardiac interactions.

Interactions between the developing heart and the embryonic immune system are essential for proper cardiac development and maintaining homeostasis, with disruptions linked to various diseases. While human pluripotent stem cell (hPSC)-derived organoids are valuable models for studying human organ function, they often lack critical tissue-resident immune cells. Here, we introduce an advanced human heart assembloid model, termed hHMA (human heart-macrophage assembloid), which fully integrates autologous cardiac tissue- resident macrophages (MPs) with pre-existing human heart organoids (hHOs).

Combination of flaxseed and clarified tomato juice in bread: Delayed effect on metabolic disorders during long-term unbalanced diet in an in vivo experiment.

Objective: The of study was to evaluate the biological effects occurring in the animal body in conditions of a regular consumption of bread with a combination of flaxseed and clarified tomato juice in its composition.

Methods: The animals (Soviet Chinchilla breed) were randomly distributed between six groups. Group 1 is intact animals.

Trophic Position Stability of Benthic Organisms in a Changing Food Web of an Arctic Fjord Under the Pressure of an Invasive Predatory Snow Crab, .

The introduction of a large predatory snow crab, , into the Kara Sea is a unique situation where the impact of an invasive species affecting an otherwise undisturbed ecosystem can be observed unhindered by other ecosystem stressors. Trophic interactions are one of the principal relationships between animals and can help assess an ecosystem's stability. The trophic positions and sources of organic material for the most common benthic species of Blagopoluchiya Bay sampled at different stages of the invasion were calculated using stable isotope analysis.

The Relevance of Spinal Muscular Atrophy Biomarkers in the Treatment Era.

Spinal muscular atrophy (SMA) is a severe neuromuscular disorder that currently has an approved treatment for all forms of the disease. Previously, biomarkers were primarily used for diagnostic purposes, such as detecting the presence of the disease or determining a specific clinical type of SMA. Currently, with the availability of therapy, biomarkers have become more valuable due to their potential for prognostic, predictive, and pharmacodynamic applications.

Serum Irisin, Myostatin, and Myonectin Correlate with Metabolic Health Markers, Liver Disease Progression, and Blood Pressure in Patients with Metabolic Dysfunction-Associated Fatty Liver Disease and Hypertension.

Background/objectives: Recent data indicate the involvement of skeletal muscles in the regulation of metabolism and in the pathogenesis of chronic noncommunicable diseases. The goal of our study was to describe the serum concentrations of myokines in patients with metabolic dysfunction-associated steatotic liver disease (MASLD) and hypertension (HTN) and their correlation with laboratory parameters, blood pressure (BP), and MASLD severity.

Methods: A total of 67 patients with MASLD and HTN underwent anthropometric measurements, laboratory tests, and point shear-wave elastography.

Wavelet-Detected Changes in Nocturnal Brain Electrical Activity in Patients with Non-Motor Disorders Indicative of Parkinson's Disease.

Background/Objectives-Parkinson's disease (PD) is the second most common neurodegenerative disorder caused by the destruction of neurons in the substantia nigra of the brain. Clinical diagnosis of this disease, based on monitoring motor symptoms, often leads to a delayed start of PD therapy and control, where over 60% of dopaminergic nerve cells are damaged in the brain substantia nigra. The search for simple and stable characteristics of EEG recordings is a promising direction in the development of methods for diagnosing PD and methods for diagnosing the preclinical stage of PD development.

Changes in the Diet of an Invasive Predatory Crab, , in the Degrading Benthic Community of an Arctic Fjord.

The introduction of a new species can lead to substantial changes in a new ecosystem. Local and introduced species' survival depends on their ability to adapt to the new environment. Studying such adaptations is often hindered by multiple factors affecting the ecosystem.

Bioelectrical Impedance Analysis Demonstrates Reliable Agreement with Dual-Energy X-ray Absorptiometry in Identifying Reduced Skeletal Muscle Mass in Patients with Metabolic Dysfunction-Associated Steatotic Liver Disease and Hypertension.

Body composition (BC) affects the risk of developing metabolic dysfunction-associated steatotic liver disease (MASLD) and hypertension (HTN). Currently, dual-energy X-ray absorptiometry (DEXA) is considered the gold standard for assessing BC, even though it has some limitations, including immobility, ionizing radiation, and patient weight restrictions. The aim of the study was to evaluate the correlations of BC parameters measured by bioelectrical impedance analysis (BIA) with those measured by DEXA in patients with MASLD and HTN.

Immunometabolic cues recompose and reprogram the microenvironment around implanted biomaterials.

Circulating monocytes infiltrate and coordinate immune responses in tissues surrounding implanted biomaterials and in other inflamed tissues. Here we show that immunometabolic cues in the biomaterial microenvironment govern the trafficking of immune cells, including neutrophils and monocytes, in a manner dependent on the chemokine receptor 2 (CCR2) and the C-X3-C motif chemokine receptor 1 (CX3CR1). This affects the composition and activation states of macrophage and dendritic cell populations, ultimately orchestrating the relative composition of pro-inflammatory, transitory and anti-inflammatory CCR2, CX3CR1 and CCR2 CX3CR1 immune cell populations.

Frequency-Domain Features and Low-Frequency Synchronization of Photoplethysmographic Waveform Variability and Heart Rate Variability with Increasing Severity of Cardiovascular Diseases.

-Heart rate variability (HRV) and photoplethysmographic waveform variability (PPGV) are available approaches for assessing the state of cardiovascular autonomic regulation. The goal of our study was to compare the frequency-domain features and low-frequency (LF) synchronization of the PPGV and HRV with increasing severity of cardiovascular diseases. -Our study included 998 electrocardiogram (ECG) and finger photoplethysmogram (PPG) recordings from subjects, classified into five categories: 53 recordings from healthy subjects, aged 28.

Maslach Burnout Inventory versus Boyko's Burnout Inventory: a comparative study and methodology.

Background: In Russia, the following two questionnaires are mainly used to study the burnout syndrome: Maslach Burnout Inventory (MBI) and Boyko's Burnout Inventory (BBI). Despite the fact that the questionnaires are based on different theoretical models and composition of the scales, they evaluate essentially the same construct. A few published sources provide data on correlations between the results of measuring burnout using these methods.

Wavelet analysis of intermittent dynamics in nocturnal electrocardiography and electroencephalography data.

This paper presents the results of a study of the characteristics of phase synchronization between electrocardiography(ECG) and electroencephalography (EEG) signals during night sleep. Polysomnographic recordings of eight generally healthy subjects and eight patients with obstructive sleep apnea syndrome were selected as experimental data. A feature of this study was the introduction of an instantaneous phase for EEG and ECG signals using a continuous wavelet transform at the heart rate frequency using the concept of time scale synchronization, which eliminated the emergence of asynchronous areas of behavior associated with the "leaving" of the fundamental frequency of the cardiovascular system.

Observation of Strong Nuclear Suppression in Exclusive J/ψ Photoproduction in Au+Au Ultraperipheral Collisions at RHIC.

We report a measurement of exclusive J/ψ and ψ(2s) photoproduction in Au+Au ultraperipheral collisions at sqrt[s_{NN}]=200  GeV using the STAR detector. For the first time, (i) the ψ(2s) photoproduction in midrapidity at the Relativistic Heavy-Ion Collider has been experimentally measured; (ii) nuclear suppression factors are measured for both the coherent and incoherent J/ψ production. At average photon-nucleon center-of-mass energy of 25.

Schiff Bases Derived from Pyridoxal 5'-Phosphate and 2-X-Phenylamine (X = H, OH, SH): Substituent Effects on UV-Vis Spectra and Hydrolysis Kinetics.

Schiff bases are compounds that are widely distributed in nature and have practical value for industry and biomedicine. Another important use of Schiff bases is identifying metal ions and different molecules, including proteins. Their proneness to hydrolysis limits the utilization of Schiff bases to mainly non-aqueous solutions.

Review on the Use of Brain Computer Interface Rehabilitation Methods for Treating Mental and Neurological Conditions.

This review provides a comprehensive examination of recent developments in both neurofeedback and brain-computer interface (BCI) within the medical field and rehabilitation. By analyzing and comparing results obtained with various tools and techniques, we aim to offer a systematic understanding of BCI applications concerning different modalities of neurofeedback and input data utilized. Our primary objective is to address the existing gap in the area of meta-reviews, which provides a more comprehensive outlook on the field, allowing for the assessment of the current landscape and developments within the scope of BCI.

Full-Term and Preterm Newborns Differ More Significantly in Photoplethysmographic Waveform Variability than Heart Rate Variability.

Background: Features of cardiovascular autonomic regulation in infants are poorly studied compared with adults. However, the clinical significance of autonomic dysfunction in infants is very high. The goal of our research was to study the temporal and frequency-dependent features, as well as low-frequency synchronization in cardiovascular autonomic regulation in full-term vs.

Immune niches for hair follicle development and homeostasis.

The hair follicle is a dynamic mini-organ that has specialized cycles and architectures with diverse cell types to form hairs. Previous studies for several decades have investigated morphogenesis and signaling pathways during embryonic development and adult hair cycles in both mouse and human skin. In particular, hair follicle stem cells and mesenchymal niches received major attention as key players, and their roles and interactions were heavily revealed.

Shedding Light on Heavy Metal Contamination: Fluorescein-Based Chemosensor for Selective Detection of Hg in Water.

This article discusses the design and analysis of a new chemical chemosensor for detecting mercury(II) ions. The chemosensor is a hydrazone made from 4-methylthiazole-5-carbaldehyde and fluorescein hydrazide. The structure of the chemosensor was confirmed using various methods, including nuclear magnetic resonance spectroscopy, infrared spectroscopy with Fourier transformation, mass spectroscopy, and quantum chemical calculations.

Assessment of Nuclear Gem Quantity for Evaluating the Efficacy of Antisense Oligonucleotides in Spinal Muscular Atrophy Cells.

Spinal muscular atrophy is a neuromuscular disorder caused by mutations in both copies of the survival motor neuron gene 1 (), which lead to reduction in the production of the SMN protein. Currently, there are several therapies that have been approved for SMA, with many more undergoing active research. While various biomarkers have been proposed for assessing the effectiveness of SMA treatment, a universally accepted one still has not been identified.

Establishment of a Pilot Newborn Screening Program for Spinal Muscular Atrophy in Saint Petersburg.

Spinal muscular atrophy 5q (SMA) is one of the most common neuromuscular inherited diseases and is the most common genetic cause of infant mortality. SMA is associated with homozygous deletion of exon 7 in the gene. Recently developed drugs can improve the motor functions of infants with SMA when they are treated in the pre-symptomatic stage.

ER stress and lipid imbalance drive diabetic embryonic cardiomyopathy in an organoid model of human heart development.

Congenital heart defects are the most prevalent human birth defects, and their incidence is exacerbated by maternal health conditions, such as diabetes during the first trimester (pregestational diabetes). Our understanding of the pathology of these disorders is hindered by a lack of human models and the inaccessibility of embryonic tissue. Using an advanced human heart organoid system, we simulated embryonic heart development under pregestational diabetes-like conditions.