[Changes in the activity of endonucleolysis of nuclear DNA in children with acute leukemia].

The previously established fact of low activity of Ca, Mg-dependent endonucleolysis of cell nucleus DNA in lymphoproliferative diseases (CME-activity) brought the authors to study intranuclear characteristics of lymphoid cells in childhood acute lymphoblastic leukemia (ALL). The intensity of DNA-endonucleolysis was measured in 0.7% agarose gel using electrophoresis.

[Changes in Ca, Mg-dependent endonuclease of DNA in isolated nuclei of human lymphocytes in lymphoproliferative diseases].

Ca, Mg-dependent endonuclease is one of the main DNAses of lymphocyte chromatin. It's activity is known to increase in the immune response and to decrease in spontaneous and experimental CLL. These observations became a basis for analysis of possible clinical meaning of it's enzymatic activity assay.

[Characteristics of the mechanisms of the immune response in typhoid fever and the bacterial carrier state].

During the acute period of typhoid fever, as well as in the early and, partially, late convalescence periods, a considerable increase in the level of T-lymphocytes and their helper activity and, in some cases, in the number of B- and D-cells and the populations of neutrophils with receptors for C3 and Fc IgG was observed. In chronic carriers immune shifts of a different character have been recorded: an increase in suppressor activity and a decrease in the number of the above-mentioned neutrophils. These data prompt the use of immunological criteria for the prediction of chronic carrier state in typhoid fever.