Silymarin attenuates benzo(a)pyrene induced toxicity by mitigating ROS production, DNA damage and calcium mediated apoptosis in peripheral blood mononuclear cells (PBMC).

Benzo(a)pyrene (B(a)P), which is the most studied member of PAH family is released into the environment (air, water and soil) from natural and man-made sources including industrial and automobile exhaust fumes. Since B(a)P is an omnipresent environmental pollutant and is believed to be a risk factor for human chemical carcinogenesis, it is important to identify potent naturally occurring/synthetic agents that could modulate B(a)P-induced toxicity. The present study explores the effect of the flavonoid silymarin (2.

Hydroxytyrosol, the phenolic compound of olive oil protects human PBMC against oxidative stress and DNA damage mediated by 2,3,7,8-TCDD.

The protective effect of hydroxytyrosol (HT), a strong antioxidant compound from extra virgin olive oil, against TCDD induced toxicity was investigated in human peripheral blood mononuclear cells (PBMC). PBMC (1 × 10(6)cellsmL(-1)) were divided into four groups and were incubated in a CO(2) incubator (5% CO(2)) for 12h with vehicle, TCDD (10 nM), TCDD+HT (10 nM+100 μM) and HT alone (100 μM) respectively. To clarify the role of HT against TCDD induced cytotoxicity, oxidative stress and the levels of antioxidant enzymes were assessed.

Olive oil protects rat liver microsomes against benzo(a)pyrene-induced oxidative damages: an in vitro study.

Benzo(a)pyrene (B(a)P), a member of the polycyclic aromatic hydrocarbon family is present ubiquitously in the environment. One of its toxic effects is induction of oxidative stress (mediated by the enzyme B(a)P hydroxylase) which leads to various diseases like cancer. Olive oil (OO) that consists of many antioxidant compounds is reported to have many beneficial properties including protection against cancer.