Publications by authors named "Kirsty M Rooney"
Article Synopsis
- The study investigates HNF1B, a gene that encodes a transcription factor important for kidney development, and its mutations that lead to kidney malformations.
- Researchers created kidney organoids with HNF1B mutations using CRISPR-Cas9 technology to study the resulting abnormalities at a cellular level.
- Findings revealed that these mutant organoids had deformed tubules and altered gene expression affecting key pathways, contributing to a better understanding of how HNF1B mutations cause kidney disease.
Optogenetics is a rapidly advancing technology combining photochemical, optical, and synthetic biology to control cellular behavior. Together, sensitive light-responsive optogenetic tools and human pluripotent stem cell differentiation models have the potential to fine-tune differentiation and unpick the processes by which cell specification and tissue patterning are controlled by morphogens. We used an optogenetic bone morphogenetic protein (BMP) signaling system (optoBMP) to drive chondrogenic differentiation of human embryonic stem cells (hESCs).
View Article and Find Full Text PDFBackground: Kidney disease causes major suffering and premature mortality worldwide. With no cure for kidney failure currently available, and with limited options for treatment, there is an urgent need to develop effective pharmaceutical interventions to slow or prevent kidney disease progression.
Summary: In this review, we consider the feasibility of using human pluripotent stem cell-derived kidney tissues, or organoids, to model genetic kidney disease.
MicroRNAs (miRNAs) are gene expression regulators and they have been implicated in acquired kidney diseases and in renal development, mostly through animal studies. We hypothesized that the miR-199a/214 cluster regulates human kidney development. We detected its expression in human embryonic kidneys by in situ hybridization.
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