Kinetics of Apoptosis of Peripheral Blood Leukocytes in Normal State and in Children Recovered from Acute Lymphoblastic Leukemia.

The original method is proposed for the study of apoptosis kinetics in cell populations by determination of apoptotic cell accumulation during the day's incubation in serum free medium. We have studied the features of this process for peripheral blood granulocytes and lymphocytes from healthy children. High sensitivity of the proposed method has been shown for the estimation of apoptosis, taking for illustration the analysis of delayed chemotherapy influence on blood cells in children, recovered from acute limphoblastic leukemia (ALL).