Environment modulates protein heterogeneity through transcriptional and translational stop codon readthrough.

Stop codon readthrough events give rise to longer proteins, which may alter the protein's function, thereby generating short-lasting phenotypic variability from a single gene. In order to systematically assess the frequency and origin of stop codon readthrough events, we designed a library of reporters. We introduced premature stop codons into mScarlet, which enabled high-throughput quantification of protein synthesis termination errors in E.

[Factors Affecting the Stability of the Trimer of 2'-Deoxyuridine 5'-Triphosphate Nucleotide Hydrolase from Escherichia coli].

2'-Deoxyuridine 5'-triphosphate nucleotide hydrolase (Dut) hydrolyzes dUTP to dUMP and pyrophosphate to prevent erroneous incorporation of dUMP from the dUTP metabolic pool into DNA. Dut is considered as a promising pharmacological target for antimetabolite therapy. Enzymatically active Dut is a trimer that binds the substrate at the interface between the subunits.

A model of the optimal selection of crypto assets.

We propose a modelling framework for the optimal selection of crypto assets. We assume that crypto assets can be described according to two features: (technological) and (governance). We simulate optimal selection decisions of investors, being driven by (i) their attitudes towards assets' features, (ii) information about the adoption trends, and (iii) expected future economic benefits of adoption.

On the formation of Dodd-Frank Act derivatives regulations.

Following the 2007-2009 financial crisis, governments around the world passed laws that marked the beginning of new period of enhanced regulation of the financial industry. These laws called for a myriad of new regulations, which in the U.S.

Tourism research from its inception to present day: Subject area, geography, and gender distributions.

This paper uses text data mining to identify long-term developments in tourism academic research from the perspectives of thematic focus, geography, and gender of tourism authorship. Abstracts of papers published in the period of 1970-2017 in high-ranking tourist journals were extracted from the Scopus database and served as data source for the analysis. Fourteen subject areas were identified using the Latent Dirichlet Allocation (LDA) text mining approach.

Comparing CMIP-3 and CMIP-5 climate projections on flooding estimation of Devils Lake of North Dakota, USA.

Background: Water level fluctuations in endorheic lakes are highly susceptible to even slight changes in climate and land use. Devils Lake (DL) in North Dakota, USA is an endorheic system that has undergone multi-decade flooding driven by changes in regional climate. Flooding mitigation strategies have centered on the release of lake water to a nearby river system through artificial outlets, resulting in legal challenges and environmental concerns related to water quality, downstream flooding, species migration, stakeholder opposition, and transboundary water conflicts between the US and Canada.

Data for Sochi 2014 Olympics discussion on social media.

Presented data is related to the research article "Sochi 2014 Olympics on Twitter: Perspectives of Hosts and Guests" [2].  The data were collected through regular API Twitter search for five months windowing 2014 Sochi Olympic Games and further used for cluster analysis and analysis of the sentiment on the Games. The main dataset contains 616 thousand tweets, rigorously cleaned and filtered to remove irrelevant content.

Inter-Coder Agreement in One-to-Many Classification: Fuzzy Kappa.

Content analysis involves classification of textual, visual, or audio data. The inter-coder agreement is estimated by making two or more coders to classify the same data units, with subsequent comparison of their results. The existing methods of agreement estimation, e.

[On the development of a system of medical weather forecast for the Caucasian Mineral Waters spa-and-resort complex].

A system of medical weather forecast for the Caucasian Mineral Waters spa-and-resort complex has been modified and updated based on the results of long-term observations of weather conditions in the region of interest with special reference to the bioclimatic regime, atmospheric circulation, aerosol pollution of the near-ground air, ultraviolet radiation, heliomagnetic activity, and meteopathic effects. This system provides a basis for the timely emergency meteopreventive treatment of meteodependent patients and therefore can be instrumental in enhancing efficiency of spa-and-resort rehabilitative therapy.

Biochemical characterization and expression analysis of a novel EF-hand Ca2+ binding protein calmyrin2 (Cib2) in brain indicates its function in NMDA receptor mediated Ca2+ signaling.

Calmyrin2 (CaMy2, Cib2) is a novel EF-hand calcium-binding protein found recently in skeletal muscles. CaMy2 mRNA was also detected in brain, but nothing is known about CaMy2 protein localization and properties in the brain. We report cloning and characterization of CaMy2 in rat brain: its expression pattern, intracellular localization and biochemical features.

Climate change impacts on forestry.

Changing temperature and precipitation pattern and increasing concentrations of atmospheric CO(2) are likely to drive significant modifications in natural and modified forests. Our review is focused on recent publications that discuss the changes in commercial forestry, excluding the ecosystem functions of forests and nontimber forest products. We concentrate on potential direct and indirect impacts of climate change on forest industry, the projections of future trends in commercial forestry, the possible role of biofuels, and changes in supply and demand.

[Diagnosis, treatment and prophylaxis of acute ulcero-erosive gastroduodenal bleedings at cardiosurgical patients].

Results of treatment of 495 patients who has undergone various cardiac operations with artificial circulation are analyzed. The complex program "Blood-free surgery" was used at 388 (78.4%) of them.

Role of ionization of the phosphate cosubstrate on phosphorolysis by purine nucleoside phosphorylase (PNP) of bacterial (E. coli) and mammalian (human) origin.

Kinetics of the reactions of purine nucleoside phosphorylases (PNP) from E. coli (PNP-I, the product of the deoD gene) and human erythrocytes with their natural substrates guanosine (Guo), inosine (Ino), a substrate analogue N(7)-methylguanosine (m(7)Guo), and orthophosphate (P(i), natural cosubstrate) and its thiophosphate analogue (SP(i)), found to be a weak cosubstrate, have been studied in the pH range 5-8. In this pH range Guo and Ino exist predominantly in the neutral forms (pK(a) 9.

Effects of mutagenesis of W343 in human annexin A6 isoform 1 on its interaction with GTP: nucleotide-induced oligomer formation and ion channel activity.

Accumulated experimental evidence suggests that annexin A6 (AnxA6) is involved in ion transport in various tissues. Such a biological function is related either to the modulation of ion transport systems by AnxA6 or to the ion channel activity of the protein. While AnxA6 channel activity at low pH seems to be associated with a large conformational transition in the protein, the mechanism of GTP-induced ion channel formation remains obscure.

Structure of human annexin a6 at the air-water interface and in a membrane-bound state.

We postulate the existence of a pH-sensitive domain in annexin A6 (AnxA6), on the basis of our observation of pH-dependent conformational and orientation changes of this protein and its N- (AnxA6a) and C-terminal (AnxA6b) halves in the presence of lipids. Brewster angle microscopy shows that AnxA6, AnxA6a, and AnxA6b in the absence of lipids accumulate at the air-water interface and form a stable, homogeneous layer at pH below 6.0.

A putative consensus sequence for the nucleotide-binding site of annexin A6.

Reaction-induced infrared difference spectroscopy (RIDS) has been used to investigate the nature of interactions of human annexin A6 (ANXA6) with nucleotides. RIDS results for ANXA6, obtained after the photorelease of GTP-gamma-S, ATP, or P(i) from the respective caged compounds, were identical, suggesting that the interactions between the nucleotide and ANXA6 were dominated by the phosphate groups. Phosphate-induced structural changes in ANXA6 were small and affected only seven or eight amino acid residues.

GTP-binding properties of the membrane-bound form of porcine liver annexin VI.

Annexin VI (AnxVI) of molecular mass 68-70 kDa belongs to a multigenic family of ubiquitous Ca2+- and phospholipid-binding proteins. In this report, we describe the GTP-binding properties of porcine liver AnxVI, determined with a fluorescent GTP analogue, 2'-(or 3')-O-(2,4,6-trinitrophenyl)guanosine 5'-triphosphate (TNP-GTP). The optimal binding of TNP-GTP to AnxVI was observed in the presence of Ca2+ and asolectin liposomes, as evidenced by a 5.

GTP-induced membrane binding and ion channel activity of annexin VI: is annexin VI a GTP biosensor?

Annexin VI (AnxVI) formed ion channels in planar lipid bilayers that were induced by the addition of millimolar guanosine 5'-triphosphate (GTP) at pH 7.4 and that were not accompanied by a penetration of the protein into the membrane hydrophobic region. GTP-influenced interactions of AnxVI with Ca2+/liposomes produced small structural alterations as revealed by circular dichroism and infrared spectroscopies.

[Methods to map traffic noise in cities].

The authors suggest a method evaluating acoustic situation on traffic pathways with restricted volume of direct measurements. The regression equations obtained could forecast equivalent noise levels changing with city growth and with shifts of traffic flow between routes.

N- and C-terminal halves of human annexin VI differ in ability to form low pH-induced ion channels.

Human recombinant annexin VI (AnxVI) or its N- (AnxVIA) and C-terminal (AnxVIB) fragments were expressed in E. coli. Their ability to form voltage-dependent ion channels in membranes, induced by low pH, was measured to verify the hypothesis that, upon acidification, the hydrophobicity of AnxVI at a specific domain significantly increases allowing the AnxVI interaction with lipids in a Ca(2+)-independent manner.

[Population-demographic structure of the population of Kursk region: anthropometric profile of newborn children].

Based on the characteristics of body height and weight in 4905 newborns, the population-genetic structure of the rural raions (districts) of Kursk oblast (region) and in the city of Kursk was determined. An "adaptive norm area" with respect to body weight and height was distinguished for newborns in Kursk oblast. On the average, the anthropometric parameters of 20% of infants from the studied populations fell within this area.

[Distribution of ABO and Rhesus phenotypes and genes in the population of Kursk region].

The distributions of AB0 and Rhesus phenotypes and the corresponding genes in the population of Kursk oblast were studied. Based on these data, genetic differentiation of rural populations with respect to the d gene frequency was revealed. The differentiation was determined by the differences in the genetic and demographic structure of these rural populations.