Thrombin Immobilized Hemocompatible Radiopaque Polyurethane Microspheres for Topical Blood Coagulation.

Over the past decade, there has been growing interest in developing microspheres for embolization procedures. However, the lack of noninvasive monitoring of the embolic agents and the occurrence of reflux phenomenon leading to unintentional occlusions has raised concerns regarding their compatibility/suitability for embolization therapy. Here we report the development of specialty microspheres having intrinsic radiopacity and surface functionality to tackle the existing complications that pave the way for more advanced solutions.

Marker counter-selection via CRISPR/Cas9 co-targeting for efficient generation of genome edited avian cell lines and germ cells.

Efficient isolation of genetically modified cells that are phenotypically indistinguishable from the unmodified cells remains a major technical barrier for the broader utilization of CRISPR/Cas9. Here, we report a novel enrichment approach to select the genome engineered cells by co-targeting a genomically integrated GFP gene along with the endogenous gene of interest (GOI). Using this co-targeting approach, multiple genomic loci were successfully targeted in chicken (DF1) and quail (CEC-32) fibroblast cell lines by transient transfection of Cas9 and guide RNAs (gRNAs).