Publications by authors named "Kiplagat Kotut"

Hot springs and saline-alkaline lakes of East Africa are extreme habitats regarding temperature, or salinity and pH, respectively. This study examines whether divergent habitats of Lake Bogoria, Kenya, impacts cyanobacterial community structure. Samples from the hot springs, pelagic zone and sediment were analysed by light microscopy, multilocus 454-amplicons sequencing and metagenomics to compare the cyanobacterial diversity.

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PCR-based denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments was used to identify the cyanobacterial phylotypes in sediments and plankton of saline-alkaline and freshwater lakes of Kenya. The detection of the aminotransferase domain located on modules mcyE and ndaF using specific molecular markers confirmed the presence of potential toxin-producing cyanobacteria. The eight nucleotide sequences obtained from DGGE bands were placed in three divergent cyanobacterial clusters.

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Phytoplankton biodiversity studies in Kenya's standing waters were carried out between 2001 and 2003. Toxin producing cyanobacteria were recorded in twelve water bodies. Microcystis and Anabaena were the most common species in freshwaters while Anabaena and Anabaenopsis were common in alkaline saline lakes.

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Article Synopsis
  • The research studied cyanobacterial mats in hot springs near Lake Bogoria, Kenya, focusing on the types of species present and the toxicity levels of the cyanobacteria.
  • The cyanobacterial mats contained various toxins, including microcystins and anatoxin-a, with concentrations suggesting potential hazards; microcystins ranged from 221 to 845 microg, while anatoxin-a levels were between 10 and 18 microg in dry weight of the mats.
  • The presence of these toxins in the stomach contents of Lesser Flamingos, along with observed neurological symptoms in the birds, indicates that the ingestion of cyanobacterial cells from the mats may be linked to the mass mortality of these flamingos.
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