Inference of weak-form partial differential equations describing migration and proliferation mechanisms in wound healing experiments on cancer cells.

Targeting signaling pathways that drive cancer cell migration or proliferation is a common therapeutic approach. A popular experimental technique, the scratch assay, measures the migration and proliferation-driven cell closure of a defect in a confluent cell monolayer. These assays do not measure dynamic effects.

Castellvi classification of lumbosacral transitional vertebrae: comparison between conventional radiography, CT, and MRI.

Background: The reliability and diagnostic accuracy of commonly used diagnostic imaging modalities in the classification of lumbosacral transitional vertebrae (LSTV) are poorly known, and comparative studies are scarce.

Purpose: To compare the diagnostic performance of conventional radiography (CR), computed tomography (CT), and magnetic resonance imaging (MRI) in classifying LSTVs.

Material And Methods: In this retrospective cross-sectional study, a total of 852 patients undergoing lumbar imaging studies using all three modalities were initially assessed for the presence of LSTV using CT scans.

Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling.

Engineering metabolism to efficiently produce chemicals from multi-step pathways requires optimizing multi-gene expression programs to achieve enzyme balance. CRISPR-Cas transcriptional control systems are emerging as important tools for programming multi-gene expression, but poor predictability of guide RNA folding can disrupt expression control. Here, we correlate efficacy of modified guide RNAs (scRNAs) for CRISPR activation (CRISPRa) in E.

Seasonal variation in metabolic profiles and microbial communities in a subarctic ore processing plant.

The mining industry strives to reduce its water footprint by recycling water in ore processing. This leads to build-up of ions, flotation chemicals and microbial biomass, which may affect the process. The Boliden Kevitsa mine in Northern Finland is exposed to seasonal change and recycles up to 90% of the process water.

Impact of NHOH treatment on the ion exchange and pore characteristics of a metakaolin-based geopolymer.

We investigated the viability and influence of NHOH post-synthetic treatment on the pore characteristics of geopolymers. Geopolymers are a class of materials with amorphous aluminosilicate three-dimensional frameworks, regarded as amorphous analogues of zeolites. Similar to zeolites, when geopolymers are used in catalysis or adsorption applications, post-synthetic treatments such as ion exchange with NH salts (, NHCl and NHNO) and desilication (using strong bases such as NaOH) are necessary to introduce active sites and modify their pore structure, respectively.

Characterizing heterogeneous single-cell dose responses computationally and experimentally using threshold inhibition surfaces and dose-titration assays.

Single cancer cells within a tumor exhibit variable levels of resistance to drugs, ultimately leading to treatment failures. While tumor heterogeneity is recognized as a major obstacle to cancer therapy, standard dose-response measurements for the potency of targeted kinase inhibitors aggregate populations of cells, obscuring intercellular variations in responses. In this work, we develop an analytical and experimental framework to quantify and model dose responses of individual cancer cells to drugs.

Veterinarians and zoonotic pathogens, infections and diseases - questionnaire study and case series, Finland.

Background: Veterinarians are at risk for numerous zoonotic infections. In this paper, we summarise descriptions of zoonotic infections from a questionnaire study and a series of work-related zoonotic cases, aiming to add to the knowledge on occupational zoonotic risks of veterinarians.

Methods: We collected data on zoonotic infections contracted by veterinarians in Finland in two studies:1) using a questionnaire in 2009, and 2) inviting veterinarians who had encountered an occupational zoonosis to report it in structured interviews in 2019.

Valorisation of baryte tailings for radiation shielding in plastics and nuclear waste disposal.

Baryte (BaSO) is a critical raw material with no functional recycling since the used applications are dissipative. Significant quantities of baryte end up in tailings as a side stream from the mining industry. Baryte from a secondary raw material source was used as a filler in plastics for low duty radiation shielding and as an aggregate in radiation shielding geopolymers needed for safely storing low-radioactive waste ash.

Post-vaccinal distemper-like disease in two dog litters with confirmed infection of vaccine virus strain.

Modified live canine distemper virus (CDV) vaccines are widely used and considered both safe and effective. Although there are occasional literature reports of suspected vaccine-induced disease, there are none where the vaccine strain has been identified in affected tissues. Here we describe two such cases in different litters.

Equine dermatitis outbreak associated with parapoxvirus.

Parapoxviruses (PPV) cause skin and mucous membrane lesions in several animal species, and of the five recognized PPVs, at least three are zoonotic. Equine PPV (EqPPV) is the sixth one initially described in humans in the United States and later in a severely sick horse in Finland in 2013-2015. In 2021-2022, a large-scale pustulo-vesicular pastern dermatitis outbreak occurred in horses all over Finland.

Molecular detection of pathogens from ticks collected from dogs and cats at veterinary clinics in Finland.

Background: Ticks carry microbes, some of which are pathogenic for humans and animals. To assess this One Health challenge, 342 ticks were collected from pet dogs and cats at 10 veterinary clinics in Finland as part of the European project "Protect Our Future Too".

Methods: The tick species were identified, and ticks were screened with quantitative PCR (qPCR) for tick-borne pathogens, including Borrelia burgdorferi sensu lato, Borrelia miyamotoi, Ehrlichia canis, Anaplasma spp.

World's human migration patterns in 2000-2019 unveiled by high-resolution data.

Despite being a topical issue in public debate and on the political agenda for many countries, a global-scale, high-resolution quantification of migration and its major drivers for the recent decades remained missing. We created a global dataset of annual net migration between 2000 and 2019 (~10 km grid, covering the areas of 216 countries or sovereign states), based on reported and downscaled subnational birth (2,555 administrative units) and death (2,067 administrative units) rates. We show that, globally, around 50% of the world's urban population lived in areas where migration accelerated urban population growth, while a third of the global population lived in provinces where rural areas experienced positive net migration.

Partial Genome Characterization of Novel Parapoxvirus in Horse, Finland.

We report a sequencing protocol and 121-kb poxvirus sequence from a clinical sample from a horse in Finland with dermatitis. Based on phylogenetic analyses, the virus is a novel parapoxvirus associated with a recent epidemic; previous data suggest zoonotic potential. Increased awareness of this virus and specific diagnostic protocols are needed.

Increased probability of hot and dry weather extremes during the growing season threatens global crop yields.

Although extreme weather events recur periodically everywhere, the impacts of their simultaneous occurrence on crop yields are globally unknown. In this study, we estimate the impacts of combined hot and dry extremes as well as cold and wet extremes on maize, rice, soybean, and wheat yields using gridded weather data and reported crop yield data at the global scale for 1980-2009. Our results show that co-occurring extremely hot and dry events have globally consistent negative effects on the yields of all inspected crop types.

Oscillatory ERK Signaling and Morphology Determine Heterogeneity of Breast Cancer Cell Chemotaxis via MEK-ERK and p38-MAPK Signaling Pathways.

Chemotaxis, regulated by oscillatory signals, drives critical processes in cancer metastasis. Crucial chemoattractant molecules in breast cancer, CXCL12 and EGF, drive the activation of ERK and Akt. Regulated by feedback and crosstalk mechanisms, oscillatory signals in ERK and Akt control resultant changes in cell morphology and chemotaxis.

Comparison of voluntary and forced digital leaps in higher education - Teachers' experiences of the added value of using digital tools in teaching and learning.

The study examines the benefits of digital tools in teaching and learning as experienced by university teachers in two different time periods: 1) during the controlled digital leap before the Covid-19 pandemic (2017-2019) and 2) during the emergency imposition of remote teaching in response to the lockdown aimed at containing the Covid-19 pandemic (2020). Teachers in different academic fields at a large multidisciplinary Finnish university (N1 = 303 and N2 = 265) responded to two open-ended questions as part of a broader questionnaire. The research identified four kinds of benefits related to digital teaching: (1) practical and administrative and (2) independence of time and place, implying practical and technical benefits; as well as (3) enhancing learning and (4) developing teaching, which are primarily pedagogical benefits, related to teaching and learning.

Crop Yield Loss Risk Is Modulated by Anthropogenic Factors.

High crop yield variation between years-caused by extreme shocks on the food production system such as extreme weather-can have substantial effects on food production. This in turn introduces vulnerabilities into the global food system. To mitigate the effects of these shocks, there is a clear need to understand how different adaptive capacity measures link to crop yield variability.

Characterization of an aged alkali-activated slag roof tile after 30 years of exposure to Northern Scandinavian weather.

Alkali-activated materials (AAMs) have been known as an alternative cementitious binder in construction for more than 120 years. Several buildings utilizing AAMs were realized in Europe in the 1950s-1980s. During the last 30 years, the interest towards AAMs has been reinvigorated due to the potentially lower CO footprint in comparison to Portland cement.

Computational modeling implicates protein scaffolding in p38 regulation of Akt.

Cells process environmental cues by activating intracellular signaling pathways with numerous interconnections and opportunities for cross-regulation. We employed a systems biology approach to investigate intersections of kinase p38, a context-dependent tumor suppressor or promoter, with Akt and ERK, two kinases known to promote cell survival, proliferation, and drug resistance in cancer. Using live, single cell microscopy, multiplexed fluorescent reporters of p38, Akt, and ERK activities, and a custom automated image-processing pipeline, we detected marked heterogeneity of signaling outputs in breast cancer cells stimulated with chemokine CXCL12 or epidermal growth factor (EGF).

Veterinarians as a Risk Group for Zoonoses: Exposure, Knowledge and Protective Practices in Finland.

Background: Veterinarians may encounter a variety of zoonotic pathogens in their work.

Methods: We conducted two cross-sectional questionnaire studies among veterinarians in Finland. Participants were recruited during two Annual Veterinary Congresses.

Co-optimization of therapeutic antibody affinity and specificity using machine learning models that generalize to novel mutational space.

Therapeutic antibody development requires selection and engineering of molecules with high affinity and other drug-like biophysical properties. Co-optimization of multiple antibody properties remains a difficult and time-consuming process that impedes drug development. Here we evaluate the use of machine learning to simplify antibody co-optimization for a clinical-stage antibody (emibetuzumab) that displays high levels of both on-target (antigen) and off-target (non-specific) binding.

Computational methods for characterizing and learning from heterogeneous cell signaling data.

Heterogeneity in cell signaling pathways is increasingly appreciated as a fundamental feature of cell biology and a driver of clinically relevant disease phenotypes. Understanding the causes of heterogeneity, the cellular mechanisms used to control heterogeneity, and the downstream effects of heterogeneity in single cells are all key obstacles for manipulating cellular populations and treating disease. Recent advances in genetic engineering, including multiplexed fluorescent reporters, have provided unprecedented measurements of signaling heterogeneity, but these vast data sets are often difficult to interpret, necessitating the use of computational techniques to extract meaning from the data.

Exploring Mechanisms of Hydration and Carbonation of MgO and Mg(OH) in Reactive Magnesium Oxide-Based Cements.

Reactive magnesium oxide (MgO)-based cement (RMC) can play a key role in carbon capture processes. However, knowledge on the driving forces that control the degree of carbonation and hydration and rate of reactions in this system remains limited. In this work, density functional theory-based simulations are used to investigate the physical nature of the reactions taking place during the fabrication of RMCs under ambient conditions.

Nanostructured and Advanced Designs from Biomass and Mineral Residues: Multifunctional Biopolymer Hydrogels and Hybrid Films Reinforced with Exfoliated Mica Nanosheets.

Transforming potential waste materials into high-value-added sustainable materials with advanced properties is one of the key targets of the emerging green circular economy. Natural mica (muscovite) is abundant in the mining industry, which is commonly regarded as a byproduct and gangue mineral flowing to waste rock and mine tailings. Similarly, chitin is the second-most abundant biomass resource on Earth after cellulose, extracted as a byproduct from the exoskeleton of crustaceans, fungal mycelia, and mushroom wastes.

Alkali-Activation of Synthetic Aluminosilicate Glass With Basaltic Composition.

Alkali-activated materials (AAMs) are a potential alternative to Portland cement because they can have high strength, good durability and low environmental impact. This paper reports on the structural and mechanical characteristics of aluminosilicate glass with basalt-like compositions, as a feedstock for AAMs. The alkali-activation kinetics, microstructure, and mechanical performance of the alkali activated glass were investigated.