Insertion Sequence (IS) Element-Mediated Activating Mutations of the Cryptic Aromatic β-Glucoside Utilization () Operon Are Promoted by the Anti-Terminator Protein (BglG) in .

The cryptic β-glucoside GFB () operon in () can be activated by mutations arising under starvation conditions in the presence of an aromatic β-glucoside. This may involve the insertion of an insertion sequence (IS) element into a "stress-induced DNA duplex destabilization" (SIDD) region upstream of the operon promoter, although other types of mutations can also activate the operon. Here, we show that increased expression of the gene, encoding a well-characterized transcriptional antiterminator, dramatically increases the frequency of both IS-mediated and IS-independent Bgl mutations occurring on salicin- and arbutin-containing agar plates.