Purification of human C-reactive protein by barium sulfate and preparative agarose electrophoresis.

A procedure is described for the purification of C-reactive protein (CRP) from human serum. The methods described take advantage of the barium sulfate adsorption property of CRP and the unique biophysical property of CRP migration during electrophoresis in agarose gels containing Ca2+. The purified CRP had an apparant molecular weight of 28,000 as determined by migration of the reduced protein during SDS polyacrylamide gel electrophoresis.

Sequential changes of the plasma-protein pattern in cases of hepatitis A.

The level of 21 plasma proteins was followed in Hepatitis A for two months after onset of icterus. The mean concentration of alpha 1-antitrypsin, orosomucoid, haptoglobin, C-reactive protein (CRP) and alpha 1-antichymotrypsin increased uniformely during the first week of hepatitis A. Thus, they differ from that of inoculation hepatitis earlier described.

The first human case of domestic Q fever in Sweden.

A case of Q fever in a 56-year-old man is reported. The patient had visited Mallorca six months prior to the present disease. Except for that he had not been outside Sweden for several years, thus being the first known case of Q fever acquired in this country.

Intradermal immunization with reduced doses of human diploid cell strain rabies vaccine: evaluation of antibody response by ELISA and mixed hemadsorption test.

The antibody response to the rabies human diploid cell strain vaccine was compared after pre-exposure immunization with 0.1 ml intradermal (i.d.

Aplastic anaemia in a case of hepatitis B with a high titer of hepatitis B antigen.

A high titer of hepatitis B antigen (HBsAg) was found in a patient with acute hepatitis B infection complicated by bone marrow aplasia. A possible antimitotic effect of HBsAg as an aetiological factor of the aplasia in this patient is discussed.

C-reactive protein, C3, C4 and properdin during the Jarisch-Herxheimer reaction in early syphilis.

The occurrence of plasma CRP during the febrile response after the first injection of penicillin was followed in 10 patients with early syphilis. An increase in CRP was noted after 12 hours, with a maximum after 24 hours. The appearance of this acute phase protein could not be correlated to cutaneous reaction, increased body temperature or leukocytosis, nor were baseline values of CRP correlated to clinical or serological activity of the infection.

Quantitative measurement of properdin in normal human serum by electroimmunoassay and single radial immunodiffusion.

Properdin in normal serum was measured by electroimmunoassay (EIA) and single radial immunodiffusion (SRID). Fresh sera gave much lower properdin values in SRID in gels containing Mg2+ ions. Storage of sera at 4 degrees C resulted in a gradual increase of the properdin values measured by SRID but not of those of the EIA.

In vitro binding of human C-reactive protein by some pathogenic bacteria and zymosan.

C-reactive protein (CRP) in serum from patients with an acute disease was adsorbed not only to , but also to , and could be eluted highly purified. The finding suggests a specific interaction between CRP and bacteria, an interaction possibly playing an important role in the stimulating effect of CRP on phagocytosis of several bacterial species.

The concentration of C-reactive protein in sera from healthy individuals.

C-reactive protein (CRP) in a concentration of 0.02-13.5 mg/l was regularly demonstrated in sera obtained from apparently healthy children and adults of both sexes.

Stimulating effect of C-reactive protein on phagocytosis of various species of pathogenic bacteria.

In an investigation of the relationship between the presence of C-reactive protein (CRP) and the promoting effect of the phagocytosis of leucocytes in an phagocytosis system containing living pathogenic bacteria, it was found that CRP stimulated phagocytosis of and . It appears that CRP is related to non-specific immunity.