A new, rapid and precise method for measuring the intra-cellular water content by gas chromatography and 3H-sucrose.

A new rapid method for measuring intra-cellular water content by gas chromatography and an isotopic (3H-sucrose) technique is described. Water content of circulating red cells was revealed to be 71.26 +/- 0.

Treatment of variant angina with drugs: a survey of 11 cardiology institutes in Japan.

Data from 11 cardiology institutes in Japan were examined to determine the effectiveness of drug therapy, especially with calcium antagonists, on variant angina. The subjects were 243 males and 43 females, most of whom were 40-59 years old. Coronary artery lesions were found in 92 of 162 patients (56.

Effects of macrocyclic polyamines and polymethylenediamines on reactions influenced by polyamines.

The effects of macrocyclic polyamines and polymethylenediamines on various reactions influenced by polyamines have been studied. Among the amines tested, 2,3,4,3- and 3,3,3,4-cyclic polyamines, NH2(CH2)6NH2 and NH2(CH2)8NH2 had some ability to stimulate polyphenylalanine synthesis, globin synthesis and rat liver isoleucyl-tRNA formation. The degree of stimulation was at most 40% of that obtained by polyamines.

Concepts of diffusion theory and a graphic approach to the description of the epidemic flow of contagious disease.

Concepts used to analyze sociological, geographic, and economic processes were adapted to an examination of the diffusion of contagious disease. The example used in applying these concepts was an epidemic of variola minor which continued for 12 months in an area of 1,006 square kilometers centered on the city of Bragança Paulista, Sao Paulo State (Brazil). A graphic procedure is proposed that depicts aspects of the epidemic flow of person-to-person transmission.

Clinical evaluation of antiarrhythmic effects of disopyramide by multiclinical controlled double-blind methods.

Used orally, in double-blind cross-over controlled trials conducted on 73 patients, disopyramide, a newly developed antiarrhythmic agent, was found to be more effective than an inert placebo (lactose), with a statistically significant difference, and to be as effective as quinidine sulfate in suppressing ventricular and supraventricular premature beats. In double-blind studies with 42 patients, it was also found to be as effective as quinidine sulfate in preventing the recurrence of atrial fibrillation after successful cardioversion. In non-blind studies attacks of paroxysmal supraventricular and ventricular tachycardia and transient atrial fibrillation were effectively prevented in the respective arrhythmias in more than 70% of the cases.

Effect of metildigoxin (beta-methyldigoxin) on congestive heart failure as evaluated by multiclinical double blind study.

The efficacy on congestive heart failure of metildigoxin (beta-methyldigoxin, MD), a derivative of digoxin (DX), which had a good absorption rate from digestive tract, was examined in a double blind study using a gorup comparison method. After achieving digitalization with oral MD or intravenous deslanoside in the non-blind manner, maintenance treatment was initiated and the effects of orally administered MD and DX were compared. MD was administered in 44 cases, DX in 42.

Variant form of angina pectoris: its clinical features and treatment. The 185th Luis Guerrero Memorial Lecture given at the 6th Paul Dudley White Session, University of Santo Tomas, Manila, The Philippines, January 24, 1979.

The variant form of angina pectoris (VA) is a rather common disease in Japan, and the author himself is suffering from it. Among 100 successive cases of various types of angina seen in our Department, excluding myocardial infarction and post-infarctional angina, 23 cases were VA. The brief duration, cyclic occurrence and frequent incidence of the attacks at night are considered to be the clinical characteristics.

Relationship between antigenic type of virus and antibody response in female patients with herpes genitalis.

The antigenic types of virus recovered from genital sites in cases of adult female genital herpes and antibody response in these patients were investigated. Twelve strains were isolated from 23 clinical specimens, and half the number of the isolates was classified as type 1 virus and the remaining half as type 2 virus. The results of serological typing corresponded well to biological differentiation by plaque-forming ability on chick embryo cultures.

Cytotoxic effect of the thoracic-duct-lymph incubated on lymphocytes.

It was found that lymphocytes cultured in the medium containing chyle were remarkably suppressed in responding to phytohemagglutinin as compared with the control culture without the fluid. This suppressive effect on the cell type seemed likely to be induced by cytotoxic substance produced from the fluid incubated. Various lipids were separated from the incubated-chyle by thin-layer and gas-liquid chromatographies and then their cytotoxic activity to the lymphocytes was examined.

Two cases of rubella infection with cardiac involvement.

During the prevalence of rubella infection in Japan in 1975, we experienced 2 cases complicated with cardiac involvement: a 40-year-old housewife and a 52-year-old man. These 2 cases showed enlarged cardiac silhouettes and bilateral pleural effusion on the chest X-ray films. Pericardial effusion was demonstrated by echocardiogram and cardiac RI pool scintigram.

The estimation of heart weight from routine chest X-ray films.

A formula to estimate heart weight was derived using a theory of quantification which employed the measurements of cardiac silhouette taken from routine postero-anterior projection chest X-ray film. The films obtained in 73 necropsied cases of essential hypertension, renal hypertension, mitral and aortic valvular diseases, and 62 necropsied cases with other diseases were used for derivation of the formula. The measurements of the horizontal distances from the midsternal line to the intersecting point of the lower margin of each rib along the left cardiac border, and of the greatest distance along the right cardiac border were employed; body height, age and sex were also included.

Changes in blood level of kininogen, prostaglandin E and hemodynamics during experimental acute myocardial ischemia with and without FOY-007.

Changes in kininogen, prostaglandin E and hemodynamics were studied during 2 hours after left anterior descending artery ligation in 19 anesthetized dogs with and without FOY-007, an inhibitor of kinin forming enzyme. Significant decrease in kininogen in aorta and great cardiac vein (aorta greater than great cardiac vein) and increase in prostaglandin E in great cardiac vein were observed after ligation, indicating release of kinin and prostaglandin E from ischemic area. Both kininogen and prostaglandin E changes were inhibited by FOY-007 but further decrease in cardiac output and increase in systemic vascular resistance were observed.

A rapid method for measuring the phospholipid synthetic activity of incubated lymphocytes.

A rapid and simple method for estimating the phospholipid synthetic activity of incubated lymphocytes by a Florisil column technique following the uptake of 14C-oleic acid was established. Stimulation of phospholipid synthesis by PHA and inhibitions caused by Tween 20 or Tween 80 and heating were evaluated easily with this method.