Publications by authors named "Kimoto H"

Article Synopsis
  • Fluoroquinolone antibiotics, like ciprofloxacin and norfloxacin, can be found in places like wastewater treatment plants and can harm the environment.
  • A study used an enzyme called laccase, which comes from spent mushroom waste, to break down these antibiotics in water.
  • The best results for breaking down the antibiotics happened at a pH of 4.5 and a temperature of 30°C, and the products were found to be safe for bacteria, which means they were less harmful afterwards.
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Purpose: To clarify the magnification error caused by the degree of tilt of the incisor and the elevation of the X-ray focus position, and the verification effect of magnification correction when performing vertical dual-exposure panoramic radiography.

Methods: Panoramic radiographic images of a phantom embedding 26 steel balls were taken at different heights (0, 5, 10, 15, and 20 mm) and tilt angles (0°, 10°, 20°, and 30°) to evaluate vertical magnification in each condition. Error and correlation coefficients in the vertical magnifications were calculated between the measured and theoretical magnification values.

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Purpose: To evaluate the image quality of vertical dual-exposure panoramic radiography (PR), which merges two PR images taken at different focus heights to reduce ghost images of cervical vertebrae (CV) and intervertebral spaces (IVS) in the incisor region.

Methods: PR images of an aluminum block, a CV phantom and a human head phantom were taken at 0 mm and merged with and subtracted from PR images taken at other heights (0, 5, 10, 15, and 20 mm) to create new images, e.g.

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Basal plant immune responses are activated by the recognition of conserved microbe-associated molecular patterns (MAMPs), or breakdown molecules released from the plants after damage by pathogen penetration, so-called damage-associated molecular patterns (DAMPs). While chitin-oligosaccharide (CHOS), a primary component of fungal cell walls, is most known as MAMP, plant cell wall-derived oligosaccharides, cello-oligosaccharides (COS) from cellulose, and xylo-oligosaccharide (XOS) from hemicellulose are representative DAMPs. In this study, elicitor activities of COS prepared from cotton linters, XOS prepared from corn cobs, and chitin-oligosaccharide (CHOS) from crustacean shells were comparatively investigated.

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Article Synopsis
  • - Lipopolysaccharide (LPS) is a bacterial toxin linked to fevers in humans, and a newly developed chemosensor exhibits a rapid fluorescence response to detect LPS in water at a very low concentration of 11 pM, outperforming earlier sensors.
  • - The sensor's fluorescence response is driven by various interactions, including hydrophobic and electrostatic forces, as well as the coordination between zinc(II)-dipicolylamine in the sensor and LPS's phosphate group.
  • - This innovative chemosensor has been integrated into a flow injection analysis system, achieving a high processing rate of 36 samples per hour, demonstrating its potential for real-time monitoring of LPS in water.
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A novel class of potent Na1.7 inhibitors has been discovered. The replacement of diaryl ether in compound was investigated to enhance mouse Na1.

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Zinc (Zn) is one of the trace elements, and Zn deficiency causes many adverse effects. Zn complexes are used for Zn supplementation, but there are few toxicity reports. Zn maltol (ZM) was orally administered for 4 weeks to male rats at a dose of 0, 200, 600, or 1000 mg/kg to assess its toxicity.

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A new chemiluminescence (CL) method based on the chemiluminescent reaction between sulfide and an acidic permanganate solution was used to quantify sulfide in seawater. A terbium-pipemidic acid complex was used as CL enhancer. The method was used to determine sulfide in the concentration range of 1-30 μmol/L in artificial seawater samples.

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A simple and practical method for boron detection in water is desired in various fields such as seawater desalination, water conservation, and plant production. To develop a method for detecting boron as boric acid in water, we synthesized [Ru(acac)(Hthap)] (acac = acetylacetonat ion, thap = 2',3',4'-trihydroxyacetophenonate (gallacetophenonate) ion) possessing a cis-diol moiety that interacts with boric acid. A comparison of UV-visible (UV-vis) absorption spectra measured in the presence and absence of boric acid at various pH values revealed that [Ru(acac)(Hthap)] shows the highest response to boric acid at pH 8.

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β-glucan, a plant polysaccharide, mainly exists in plant cell walls of oats, barley, and wheat. It is attracting attention due to its high potential for use as functional foods and pharmaceuticals. We have previously reported that low-molecular-weight -fermented β-D-glucan (LMW-AP-FBG) could inhibit inflammatory responses by inhibiting mitogen-activated protein kinases and nuclear factor-κB signaling pathways.

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Purpose: The dual imaging plate (DIP) method, which synthesizes intraoral radiographs from a front imaging plate (FIP) and a back imaging plate (BIP), produces adequate image quality and allows the radiation dose to be reduced. However, there are slight errors in superimposition and alignment between the FIP and BIP. The aim of this study was to establish positional correction in the DIP method and evaluate the effect.

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Objectives: Artifacts including scratches and dirt artifacts on the digital intraoral radiographs finally contribute to making inaccurate diagnoses. The aim of this study was to reduce the incidence of artifacts using dual imaging plates (DIPs) in imaging processing.

Methods: Conventional X-rays were taken of a porcine mandible embedded in acrylic resin using a DIP which consists of a front IP (FIP) and a back IP (BIP) with some scratches and dirt.

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Endotoxin is a lipopolysaccharide (LPS) that is found in the outer membrane of the cell wall of Gram-negative bacteria. Due to its high toxicity, the allowable endotoxin limit for water for injection is set at a very low value. Conventional methods for endotoxin detection are time-consuming and expensive and have low reproducibility.

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Evaluation of learning and memory is crucial in juvenile animal toxicity studies (JAS) during the development of CNS active drugs, but there are no currently recommended test methods. We compared the ability of the Morris water maze (MWM) and the Biel water maze (BWM) to detect learning and memory disorder (LMD) using rats inhaled isoflurane (IFN). Rats were treated with 1% IFN using inhalation on postnatal day (PND) 7 for 6 h.

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The vomeronasal system plays an essential role in sensing various environmental chemical cues. Here we show that mice exposed to blood and, consequently, hemoglobin results in the activation of vomeronasal sensory neurons expressing a specific vomeronasal G protein-coupled receptor, Vmn2r88, which is mediated by the interaction site, Gly17, on hemoglobin. The hemoglobin signal reaches the medial amygdala (MeA) in both male and female mice.

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Purpose: This study evaluated the contrast-to-noise ratio (CNR), spatial resolution, and subjective quality of dual imaging plates (DIP) intraoral radiography.

Methods: The DIP and conventional single IP (CSIP) methods both used YCR DT-1 imaging plates (Yoshida Co.).

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β-glucan derived from the black yeast Aureobasidium pullulans (A. pullulans) is one of the natural products attracting attention due to its high potential for application as a functional food and pharmaceutical. Our team of researchers obtained a highly soluble, low-molecular-weight β-glucan from the fermentation culture medium of A.

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Article Synopsis
  • The study examines the role of genetic variants in predicting lethal arrhythmic events in Brugada syndrome, focusing on how functional evaluation of specific SCN5A mutations can enhance prognostic accuracy.
  • Researchers evaluated 22 variants among 55 SCN5A mutations using laboratory methods, finding that carriers of loss-of-function SCN5A mutations experienced more severe heart issues and higher rates of lethal events compared to other mutation carriers.
  • The findings highlight the importance of functionally validating these genetic variants for better risk assessment, revealing that only certain SCN5A mutations are significantly linked to dangerous outcomes, while other associated genes showed no meaningful impact.
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The chitinolytic bacterium sp. str. FPU-7 efficiently degrades chitin into oligosaccharides such as -acetyl-D-glucosamine (GlcNAc) and disaccharides (GlcNAc) through multiple secretory chitinases.

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Article Synopsis
  • Strict guidelines can make it hard to tell if certain genetic changes are harmful for conditions like long QT syndrome and Brugada syndrome, leading to many unclear results.
  • Scientists compared genetic data from patients with these conditions to other population data to create better rules that help identify which genetic changes are serious.
  • Their new approach showed that they could find more harmful genetic variants in European patients, making genetic testing for these heart diseases more accurate and reliable.
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Article Synopsis
  • Mutations in nuclear envelope genes linked to Emery-Dreifuss muscular dystrophy can cause cardiac issues, but some mutations result in heart problems without skeletal symptoms.
  • A study analyzed families with cardiac conduction diseases and identified three specific mutations associated with progressive atrial arrhythmias and left ventricular noncompaction (LVNC).
  • The findings suggest that cardiac emerinopathy presents as a new condition characterized by atrial standstill, LVNC, and a heightened risk of thromboembolism, distinguishing it from traditional Emery-Dreifuss muscular dystrophy.
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Catalase has crucial role in adaptive response to HO. Main channel structure responsible for substrate selectivity was estimated to understand the relationship between the evolutionary direction of catalases from and which survive in cold and high concentration of hydrogen peroxide, and their catalytic property. .

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The discovery of a novel class of state-dependent voltage-gated sodium channel (Na)1.7 inhibitors is described. By the modification of amide or urethane bond in Na1.

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A highly potent, selective Na1.7 inhibitor, DS-1971a, has been discovered. Exploration of the left-hand phenyl ring of sulfonamide derivatives ( and ) led to the discovery of novel series of cycloalkane derivatives with high Na1.

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