Publications by authors named "Kimberly Robyak"
Objective: We validated an automated microfluidic interleukin-6 (IL-6) immunoassay on the Ella platform for clinical use.
Methods: The assay was validated for precision, lower limit of quantification, analytical measurement range, accuracy, specificity, interference of biotin, tocilizumab, GP130, IL-6R, hemolysis, icterus, and lipemia, and establishing the reference value. The clinical performance was evaluated in 96 COVID-19 patients.
Background: Recent debate on the race correction factor in creatinine-based estimated glomerular filtration rate (eGFR) has led to the development of a new race-independent equation (Chronic Kidney Disease Epidemiology Collaboration, CKD-EPI_2021). Previously, some institutions have already modified the early version of the CKD-EPI or Modification of Diet in Renal Disease (MDRD) equations by removing the race factors (CKD-EPI_2009_non-Black (NB), MDRD_NB) for Black populations although this approach remains controversial.
Methods: In this study, the CKD-EPI_2009_NB, MDRD_NB, and European Kidney Function Consortium (EKFC) equations were compared directly with the CKD-EPI_2021 equation in eGFR calculation, chronic kidney disease (CKD) diagnosis, and staging in a local population.
Article Synopsis
- The Vitamin D External Quality Assessment Scheme (DEQAS) sends human serum samples to over 1000 global participants to measure total serum 25-hydroxyvitamin D levels four times a year.
- A study was conducted to determine if shipping these samples at ambient temperature affects the reliability of various 25(OH)D assays compared to shipping them frozen.
- Results showed significant differences for four specific assays when samples were shipped ambiently, but all 14 LC-MS/MS assays showed no significant differences, indicating they remained stable during shipping at room temperature.
An interlaboratory comparison study was conducted by the Vitamin D Standardization Program (VDSP) to assess the performance of liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays used for the determination of serum total 25-hydroxyvitamin D (25(OH)D), which is the sum of 25-hydroxyvitamin D (25(OH)D) and 25-hydroxyvitamin D (25(OH)D). A set of 50 single-donor samples was assigned target values for concentrations of 25(OH)D, 25(OH)D, 3-epi-25-hydroxyvitamin D (3-epi-25(OH)D), and 24R,25-dihydroxyvitamin D (24R,25(OH)D) using isotope dilution liquid chromatography - tandem mass spectrometry (ID LC-MS/MS). VDSP Intercomparison Study 2 Part 1 includes results from 14 laboratories using 14 custom LC-MS/MS assays.
View Article and Find Full Text PDFArticle Synopsis
- - An interlaboratory study was conducted by the Vitamin D Standardization Program (VDSP) to evaluate how well Standard Reference Materials (SRMs) and proficiency testing samples can be used interchangeably for measuring serum total 25-hydroxyvitamin D levels using different assay methods.
- - A total of 50 single-donor serum samples were tested across 28 laboratories using a mix of 20 ligand binding assays and 14 LC-MS/MS methods, with target values assigned based on reference measurement procedures.
- - Results showed that certain SRM and proficiency testing samples were found to be non-commutable for specific assays, particularly indicating that SRM 972a (with high 3-epi-25(OH)
Unlabelled: Renal dysfunction is associated with poor long-term outcomes after liver transplantation. We examined the renal sparing effect of everolimus (EVR) compared to standard calcineurin inhibitor (CNI) immunosuppression with direct measurements of renal function over 24 months.
Methods: This was a prospective, randomized, open-label trial comparing EVR and mycophenolic acid (MPA) with CNI and MPA immunosuppression.
Background: In the absence of a safe, effective vaccine, the worldwide spread of COVID-19 (SARS-CoV-2) infection will continue. Laboratory tests with ideal precision, sensitivity, and specificity should be used in public health and clinical settings to gauge the extent of virus exposure. Toward this end, we evaluated the analytical and clinical performance of the Abbott SARS-CoV-2 IgG and the Roche Anti-SARS-CoV-2 immunoassays.
View Article and Find Full Text PDF