Publications by authors named "Kimberly Cornelio"
Article Synopsis
- Theonellamides (TNMs) are antifungal compounds derived from marine sponges that show increased affinity for membranes when cholesterol or ergosterol is present.
- In aqueous environments, TNM-A forms oligomers instead of remaining monomeric as it does in DMSO, and this study used NMR techniques to understand how TNM-A interacts with sterols like 25-hydroxycholesterol (25-HC).
- The findings suggest that the cholesterol complex with TNM-A makes it more hydrophobic, leading to enhanced membrane penetration and disruption, and highlights that 25-HC can effectively replace cholesterol in experimental studies.
Amphidinols are polyketide metabolites produced by marine dinoflagellates and are chiefly composed of a long linear chain with polyol groups and polyolefins. Two new homologues, amphidinols 20 (AM20, 1) and 21 (AM21, 2), were isolated from Amphidinium carterae collected in Korea. Their structures were elucidated by detailed NMR analyses as amphidinol 6-type compounds with remarkably long polyol chains.
View Article and Find Full Text PDFTheonellamide A (TNM-A) is an antifungal bicyclic dodecapeptide isolated from a marine sponge Theonella sp. Previous studies have shown that TNM-A preferentially binds to 3β-hydroxysterol-containing membranes and disrupts membrane integrity. In this study, several H NMR-based experiments were performed to investigate the interaction mode of TNM-A with model membranes.
View Article and Find Full Text PDFTheonellamides (TNMs) are antifungal and cytotoxic bicyclic dodecapeptides derived from the marine sponge Theonella sp. These peptides specifically bind to 3β-hydroxysterols, resulting in 1,3-β-D-glucan overproduction and membrane damage in yeasts. The inclusion of cholesterol or ergosterol in phosphatidylcholine membranes significantly enhanced the membrane affinity of theonellamide A (TNM-A) because of its direct interaction with 3β-hydroxyl groups of sterols.
View Article and Find Full Text PDFAim: To investigate the chemical constituents of the stems, leaves and roots of Euphorbia hirta, and to test for the cytotoxic and antimicrobial potentials of the major constituents of the plant.
Methods: The compounds were isolated by silica gel chromatography and their structures were elucidated by NMR spectroscopy. The cytotoxicity tests were conducted using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, while the antimicrobial tests employed the agar well method.