Effects of feeding expeller-extruded high oleic soybeans in broiler diets on growth performance, blood profile, and meat quality.

This study evaluated the effects of expeller-extruded high oleic soybeans (TruSoya) on growth performance, blood immune and antioxidant status, and meat quality of broilers compared with conventional soybean meal (SBM) and expeller-extruded conventional soybeans. A total of 288 one-day-old male broiler chicks (Cobb500; initial BW: 37.2 g) were allotted to one of 3 dietary treatments: 1) a corn basal diet with conventional SBM (CSBM); 2) a corn basal diet with expeller-extruded conventional soybeans (EECS); 3) a corn basal diet with expeller-extruded high oleic soybeans (EEHS) in a randomized complete block design.

Three-way comparison of BBL CHROMagar MRSA II, MRSASelect, and spectra MRSA for detection of methicillin-resistant Staphylococcus aureus isolates in nasal surveillance cultures.

Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-acquired and life-threatening infections. Active surveillance programs for MRSA utilize either molecular or culture-based methods. A prospective study was performed to compare the performance of selective and differential chromogenic media, BBL CHROMagar MRSA II (CMRSA II; BD Diagnostics, Sparks, MD), MRSASelect (Bio-Rad Laboratories, Redmond, WA), and Spectra MRSA (Remel, Lenexa, KS), for the detection of MRSA in nasal swab specimens.

Distribution of resistant gram-positive organisms across the census regions of the United States and in vitro activity of tigecycline, a new glycylcycline antimicrobial.

Background: Gram-positive isolates were collected from 76 medical centers within the 9 census regions across the United States.

Methods: Antimicrobial susceptibilities were determined according to Clinical and Laboratory Standards Institute guidelines.

Results: Vancomycin resistance among Enterococcus faecium isolates varied from 45.

Clustering of nucleosides in the presence of alkali metals: Biologically relevant quartets of guanosine, deoxyguanosine and uridine observed by ESI-MS/MS.

Electrospray ionization (ESI) mass spectra of nucleosides, recorded in the presence of alkali metals, display alkali metal ion-bound quartets and other clusters that may have implications for understanding non-covalent interactions in DNA and RNA. The tetramers of guanosine and deoxyguanosine and also their metaclusters (clusters of clusters), cationized by alkali metals, were observed as unusually abundant magic number clusters. The observation of these species in the gas phase parallels previous condensed-phase studies, which show that guanine derivatives can form quartets and metaclusters of quartets in solution in the presence of metal cations.

Clustering of nucleobases with alkali metals studied by electrospray ionization tandem mass spectrometry: implications for mechanisms of multistrand DNA stabilization.

Self-clustering of the five common nucleobases was investigated by electrospray ionization tandem mass spectrometry and shown to provide insight into the non-covalent interactions between identical bases. Alkali and ammonium cations significantly increase self-aggregation of the nucleobases and lead to the formation of uniquely stable magic number clusters. Sodium adducts of guanine, thymine and uracil preferentially take the form of tetrameric (quartet) clusters.