Publications by authors named "Kim Kley Valladares-Diestra"

Article Synopsis
  • Xylooligosaccharides (XOS) are prebiotic oligosaccharides with significant health benefits and growing market interest due to their low dosage and high value.
  • They can be sustainably produced from inexpensive lignocellulosic materials through efficient pretreatment and production methods, including autohydrolysis and enzymatic hydrolysis.
  • The review highlights the importance of purification and characterization of XOS to enhance their beneficial properties and applications, advocating for their role in the expanding industrial prebiotic market.
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Pesticides pose significant risks to both human health, such as cancer, neurological disorders, and endocrine disruption, and ecosystems, through the destruction of beneficial insects, contamination of soil and water, and impact on non-target species. In the face of escalating pesticide pollution, there is an urgent need for multifaceted approaches to address the issue. Bioremediation emerges as a potent tool in the environmental pollution mitigation arsenal.

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In recent years, concerns about a good-quality diet have increased. Food supplements such as prebiotics have great nutritional and health benefits. Within the diverse range of prebiotics, xylooligosaccharides (XOs) show high potential, presenting exceptional properties for the prevention of systemic disorders.

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Due to their widespread occurrence and the inadequate removal efficiencies by conventional wastewater treatment plants, emerging contaminants (ECs) have recently become an issue of great concern. Current ongoing studies have focused on different physical, chemical, and biological methods as strategies to avoid exposing ecosystems to significant long-term risks. Among the different proposed technologies, the enzyme-based processes rise as green biocatalysts with higher efficiency yields and lower generation of toxic by-products.

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Lignocellulosic biomass is a renewable material of great abundance. However, its recalcitrant characteristic requires the application of pretreatments. Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.

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The high costs of bioplastics' production may hinder their commercialization. Development of new processes with high yields and in biorefineries can enhance diffusion of these materials. This work evaluated the production of polyhydroxybutyrate (PHB) from the combination of milled corn starchy fraction hydrolysate and crude glycerol as substrates by the strain Cupriavidus necator LPB 1421.

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The application of biorefinery concepts to produce different value-added biomolecules such as xylooligosaccharides (XOs) generates economical competitive, sustainable and environmentally friendly processes. The objective of this work was to develop an efficient imidazole-pretreatment process of sugarcane bagasse (SB) and the use of the obtained hemicellulose fraction in the production of XOs with the application of in house produced xylanolytic enzymes using SB as substrate, under a biorefinery approach. SB imidazole pretreatment allowed the recovery of a hemicellulose rich fraction (34%) with 91.

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A biorefinery approach was applied for pectin extraction, xylooligosaccharides' (XOs) and bioethanol production from cocoa pod husk (CPH) using citric acid-assisted hydrothermal pretreatment. Under optimal conditions at 120° C, 10 min and 2% w.v of citric acid a high pectin recovery (19.

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Cocoa beans are produced through on-farm processing where residual biomass is discarded, including cocoa pod husks (CPH), cocoa bean shells and cocoa sweatings. CPH represents about 80% of these residues that are generated during the initial cocoa bean processing steps and their disposal occupies large areas, causing social and environmental concerns. In the last decades, the lignocellulosic composition of CPH has attracted the attention of the scientific and productive sector.

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The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery. CPH́s pectin recovery was improved with concomitant production of xylooligosaccharides (XOS) through efficient enzymatic hydrolysis of the solid fraction. A central composite experimental design was planned to analyze the effect of pretreatment conditions.

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The use of low-cost feedstock for enzyme production is an environmental and economic solution. Sugarcane bagasse and soybean meal are employed in this study for optimised xylanase production with the concomitant synthesis of proteases. The enzymatic complex is produced by submerged fermentation by Aspergillus niger.

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