Self-packed filter plates: a good alternative for pre-packed filter plates for developing purification processes for therapeutic proteins.

The use of high-throughput screening (HTS) has been successfully applied over the past years in downstream process development of therapeutic proteins. Different HTS applications have been introduced to speed up the development of purification processes for these proteins. In the light of these findings, studies were conducted to develop a controlled method of pipetting the right amount of resin into self-packed filter plates.

Aspergillus vadensis, a new species of the group of black Aspergilli.

A strain from the group of black Aspergilli was analysed in detail to determine the species to which it belongs. A detailed analysis of morphology, RFLP patterns and metabolite profiles was carried out. In addition, a phylogenetic tree was constructed for the black Aspergilli using the ITS and the beta-tubulin sequences of the individual strains.

A new black Aspergillus species, A. vadensis, is a promising host for homologous and heterologous protein production.

A new species of the group of black aspergilli, Aspergillus vadensis, was analyzed for its potential as a host for homologous and heterologous protein production. Unlike the other black aspergilli, this strain does not acidify the culture medium when nitrate is the nitrogen source and only produces very low levels of extracellular proteases, mainly serine metalloproteases. The stability of A.