CuZn-SOD suppresses the bovine papillomavirus-induced proliferation of fibroblasts.

Eukaryotic cells continuously produce reactive oxygen species (ROS) and have mechanisms to control ROS levels. ROS have been shown to mediate cell proliferation and transformation. We studied the effect of CuZn-superoxide dismutase (CuZnSOD) on the focus-forming ability of bovine papillomavirus (BPV-1) wtDNA and hypertransforming mutant of its major oncoprotein E5, E5-17S.

Bovine papillomavirus type 1 oncoprotein E5 stimulates the utilization of superoxide radicals in the mouse fibroblast cell line C127.

The major transforming protein of bovine papillomavirus type 1 (BPV-1) is a small hydrophobic polypeptide, the E5 gene product, localized in the cellular membranes and modulating various pathways in the cell. Many studies have shown that reactive oxygen species (ROS) are essential in several biological processes, including cell transformation by oncogenes, but unregulated ROS are highly toxic to cells. We studied the effect of the bovine papillomavirus protein E5 and its mutants on the level of the superoxide radicals in the mouse fibroblast cell line C127.

Two antioxidative lactobacilli strains as promising probiotics.

Two antioxidative strains tentatively identified as Lactobacillus fermentum, E-3 and E-18, were isolated from intestinal microflora of a healthy child. Survival time of these strains in the presence of reactive oxygen species (ROS), like hydrogen peroxide, superoxide anions and hydroxyl radicals, was significantly increased compared with a non-antioxidative strain, and also was quite similar to a highly ROS resistant strain of Salmonella typhimurium. E-3 and E-8 contain a remarkable level of glutathione, express Mn-SOD, which is important for the prevention of lipid peroxidation, and secrete hydrogen peroxide.

Physical exercise induces activation of NF-kappaB in human peripheral blood lymphocytes.

Current understanding of nuclear factor-kappaB (NF-kappaB) activation is derived mostly from in vitro studies, and in vivo human data are limited. This study provides first evidence showing that physical exercise (80% maximal O2 consumption, 1 h) may trigger NF-kappaB activation, as determined by electrophoretic mobility shift assay, in peripheral blood lymphocytes of physically fit young men. Supershift assay showed that the NF-kappaB protein complex contained the transcriptionally active p65 protein.

Bovine papillomavirus oncoprotein E5 affects the arachidonic acid metabolism in cells.

The bovine papillomavirus type 1 (BPV-1) oncoprotein encoded by the E5 ORF is a small highly hydrophobic protein, which is capable of inducing oncogenic transformation of cells. We studied the effect of the BPV-1 E5 protein expression on the arachidonic acid metabolism in monkey (COS1) and human (C33A) cells. At relatively low protein concentrations the phospholipase A(2) (PLA(2)) activity and the arachidonic acid (AA) metabolism are activated.

Bovine papillomavirus oncoprotein E5 induces the NF kappa B activation through superoxide radicals.

Bovine papillomavirus type 1 (BPV-1) open reading frame E5 encodes the smallest known oncoprotein. This protein is involved in transforming cells during the early stage of infection. Nuclear factor kappa B (NF kappa B) is activated in the host cells as a result of different virus infections.

Human CuZn superoxide dismutase enzymatic activity in cells is regulated by the length of the mRNA.

Single functional human CuZnSOD gene encodes two species of mRNA differing in size by 200 nucleotides in the 3'-untranslated region (UTR). We studied the expression of the CuZnSOD cDNA with different 3'- and 5'-UTR. Deletion in the 5'-end does not affect the expression of the enzyme, however, deletion in the 3'-UTR decreases the level of expression of CuZnSOD.