Comparison of the cholinergic system in neocortical field Ep in cats with strong and weak cognitive abilities.

Comparative analysis of the cholinergic system of the neocortex, consisting of the terminals of neurons from the magnocellular basal nuclei and intracortical neurons, in cats with strong and weak abilities to solve abstraction and generalization tasks was performed by isolating subfractions of synaptic membranes and synaptoplasm from "light" (C) and "heavy" (D) synaptosomes from associative field Ep and measuring choline acetyltransferase and acetylcholinesterase activities, protein content, and sulfhydryl group concentrations. These experiments showed that all measures were significantly lower in subfractions from C synaptosomes from cats with strong cognitive abilities. This leads to the conclusion that relatively small numbers of cholinergic synapses form in field Ep of the brains of cats with strong cognitive abilities, while their location in the C fraction demonstrates that they correspond to neurons of the magnocellular basal nuclei.

[Comparison of cholinergic systems in Ep neocortical regions of cats with high and low cognitive ability].

Cats were behaviorally tested for the ability to solve the abstraction and generalization tasks. Fractions of light (C) and heavy (D) synaptosomes of the associative temporal (Ep) areas were prepared, and subfractions of synaptic membranes and synaptoplasm were isolated. Choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activity and the content of protein and protein sulphydric (SH-) groups were measured in synaptic subfractions.