Reactive oxygen and nitrogen species in the course of B-CLL.

Purpose: The study objective was to investigate the production of NO, cGMP and superoxide anion radical by neutrophils, and to examine alterations in serum or plasma total nitric oxide, MDA and cGMP levels in B-CLL patients.

Material And Methods: PMNs were isolated from 20 patients with B-CLL. Total nitrite was measured in cell supernatants and serum by Griess method.

TRAIL receptors in the serum of patients with B-cell chronic lymphocytic leukemia.

Great importance in the course of chronic B-cell lymphocytic leukemia (B-CLL) has been ascribed to cytokines belonging to the superfamily of the tumor necrosis factor (TNF), including TRAIL (TNF-related apoptosis inducing ligand) and its specific receptors: TRAIL receptor 1 (TRAIL-R1), TRAIL receptor 2 (TRAIL-R2), TRAIL receptor 3 (TRAIL-R3), TRAIL receptor 4 (TRAIL-R4) and osteoprotegerin (OPG). Both the molecule and the receptors may occur in membrane and soluble forms, except for OPG which has only a soluble form. The aim of the study was to assess the levels of sTRAIL molecule and soluble TRAIL receptors - sTRAIL-R2 and OPG in the serum of patients with B-CLL.

[Nitric oxide in supernatants of leukocytes and the serum of patients with B-CLL].

Progression of B-cell chronic lymphocytic leukemia (B-CLL) is linked to an abnormal immune system in the host. Recent studies have suggested that polymorphonuclear neutrophils (PMN) play a role in the malignancy process through release of a wide range of mediators, involving nitric oxide (NO). The aim of this study was to examine NO production by PMN and, for comparison of peripheral blood mononuclear cell (PBMC) confronted with the expression and concentration of inducible NO synthase (iNOS) in these cells derived from patients with B-CLL.

[Estimation of L-selectin expression on neutrophils and level of soluble L-selectin form in serum of patient with chronic myelogenic leukemia].

The aim of this study was the estimation of L-selectin expression on PMN and concentration of sL-selectin in patients serum with chronic myelogenic leukemia. The results indicate the increased expression of L-selectin on isolated neutrophils from peripheral blood of patients with chronic myelogenic leukemia. A concentration of sL-selectin was also increased in patients serum with chronic myelogenic leukemia.

[Estimation of level of soluble form PECAM-1, ICAM-2 and TNF-alpha, IL-18 in serum patients with chronic myelogenic leukemia].

The aim of this study was to estimate sPECAM-1, sICAM-2 and TNF-alpha and IL-18 concentrations in serum patients with chronic myelogenic leukemia. The results indicate of increased level sPECAM-1, sICAM-2 and TNF-alpha, IL-18 concentrations in serum patients with chronic myelogenic leukemia. Elevation levels of sPECAM-1 and sICAM-2 may lead to inhibit of making myelogenic leukemia cells infiltrations through the block of surface their receptors in patients with CML.

Soluble form of TRAIL, Fas and FasL in the serum of patients with B-CLL.

Purpose: Although many studies demonstrated expression of TNF family members in the course of B-CLL, there is a little known about relationships between soluble forms of these proteins. Furthermore, there is no study reported on effects of used therapy on this relation. The present study was designed to asses the relationships between the serum concentrations of sFas, sFasL and sTRAIL in patients with B-CLL regarding their correlation with clinical stage and used therapy.

TNF family molecules in the serum of patients with B-cell chronic lymphocytic leukemia (B-CLL).

Tumor necrosis factor (TNF) superfamily, involving membrane receptors and ligands are important for the growth and survival of leukemic B cells. In the present study levels of TNF-alpha, sTNFRp55, sTNFRp75 and sCD40 and sCD40L in the serum of patients with B-CLL before and after treatment were measured. In sera of patients before treatment increased concentrations of sCD40 and decreased concentrations of sCD40L were shown.

The value of estimating serum aproptotic marker concentrations in monitoring and prognosis of 131I--therapy in Graves' disease. Preliminary report.

Background: The effect of radioiodine (131I) in Graves' disease (GD) is probably due to the direct physical destruction of thyrocytes by beta radiation, and by the indirect action through stimulation of apoptosis in these cells. The aim of our study was to investigate the changes in serum concentrations of sFas and sFasL as stimulators of apoptosis, and Bcl-2 as an inhibitor of apoptosis in patients with GD following 131I administration.

Material And Methods: The study was performed on 30 patients with GD (29 female and 1 male aged 25-45).

[Effect of cytostatic therapy on IL-1beta and L-selectin concentrations, synthesis, accumulation and excretion in patients with acute myeloid leukaemia].

The etiology and pathogenesis of the majority of diseases that make up the acute leukemias is unknown. A change in IL-1beta and L selectin concentrations is most likely to occur in the course of subtype M2 of acute myeloid leukaemia (AML). The purpose of our study was to examine the change in concentrations of these molecules mentioned above in blood plasma, culture supernatant and isolated, broken granulocytes in AML patients in both exacerbation and remission of the disease and in healthy control group.

[Blood levels of the adhesion molecules ICAM-1 and VCAM-1 in patients with chronic myeloid leukemia during remission and exacerbation].

A change in ICAM-1 and VCAM-1 concentrations is most likely to occur during the course of chronic myleoid leukemia (CML). The purpose of our study was to examine concentrations of these adhesion molecules in blood plasma, culture supernatant and isolated, broken granulocytes in 20 patients (45-65 years old) with CML in exacerbation and during the remission of the disease and in 10 healthy control subjects. The concentration assay of substances mentioned above was made using ready immunoenzymatic sets of ELISA type.

[Interleukin-6 and interleukin-12 blood levels in patients with chronic B-cell lymphocytic leukemia].

B-cell chronic lymphatic leukaemia (B-CLL) is characterized by proliferation and accumulation of small B-lymphocytes, which are monoclonal in organ. The changes in IL-6 and IL-12 concentrations usually occur during the course of B-CLL. IL-6 and IL-12 seem to be positive kinetic regulators of stem cells.

[Study of interleukins and their soluble receptors in B-cell chronic lymphatic leukemia].

The purpose of our study was to examine concentrations of IL-1 beta, IL-1 beta R, IL-6 and IL-6R in blood plasma, culture supernatant and isolated broken lymphocytes in 20 patients with CLL, at I and III stage of the disease according to Rai's classification and in 10 healthy control subjects. The studies were carried out on the cultures of cells nonstimulated and stimulated with mitogene. A significant increase in IL-1 beta and IL-6 concentrations were found in all study groups during the course of B-CLL.

[sCD44 in peripheral blood cultures of mononuclear cells and in the serum of patients with B-cell chronic lymphocytic leukemia].

The aim of this study was to estimate the ability of peripheral blood mononuclear cells to release of soluble CD44 standard (sCD44st) compared to concentration in the serum of patients B cell chronic lymphocytic leukemia. The results showed the decrease of secretion of sCD44 by leukaemic cells in studied patients. There was no significant differences between patients with I and III stage according to Rai classification.

[Effect of LFA-1 expression on neutrophils and serum level of soluble ICAM-1 in patients with chronic myelogenous leukemia].

The aim of this study was to estimation of LFA-1 expression on PMN and concentration of sICAM-1 in serum patients with chronic myelogenic leukemia. The results indicate on increased expression of LFA-1 on isolated neutrophils from peripheral blood of patients with chronic myelogenic leukemia. A concentration of sICAM was increased in serum patients with chronic myelogenic leukemia either.

[Chemokines and their participation in leukemic cells migration].

Impaired migration of leukocytes is characteristic feature of leukaemias. Knowledge of the mechanisms of leukaemia cells migration has expanded greatly in recent years. Leukocytes infiltrates are formed in surrounding tissues due to changes in chemokines and adhesion molecules concentrations.

[Assessment of serum levels of adhesion molecules (sICAM-1, sVCAM-1, sE-selectin) in stable and unstable angina and acute myocardial infarction].

The targeting and recruitment of inflammatory cells to vascular endothelium in ischaemic heart diseases is mediated by Intercellular Adhesion Molecule-1 (ICAM-1), Vascular Cell Adhesion Molecule (VCAM-1) and E-selectin and proinflammatory cytokines. Accumulation of mononuclear cells to the endothelium is one of the earliest events in the formation of an atherosclerotic lesion. The aim of this study was to estimate the serum concentrations of soluble intercellular adhesion molecule-1 (sICAM-1), vascular adhesion molecule-1 (sVCAM-1), selectin E (sE-selectin) in patients with acute myocardial infarction (Group 1--n = 18 patients: 3 women and 15 men, mean age--60 years), unstable angina pectoris (Grupa 2--n = 31 patients: 8 women and 23 men, mean age--62 years and stable heart disease (Grupa 3--n = 25 patients: 14 women and 11 men, mean age--61 years.

Cytokine and adhesion molecule concentrations in blood of patients with B-cell chronic lymphocytic leukaemia with regard to disease progression.

Purpose: To examine concentrations of IL-8, E-selectin and VCAM-1 in blood plasma, culture supernatant and isolated broken lymphocytes in patients with chronic lymphocytic leukaemia (CLL), at I and III stage of the disease according to Rai's classification and in healthy individuals constituting controls. Two types of lymphocyte cultures were carried out--nonstimulated and stimulated with mitogen.

Material And Methods: A concentration assay of substances mentioned above was made using ready immuno-enzymatic sets of the ELISA type.

Cytokines in patients with ischaemic heart disease or myocardial infarction.

Background: Cytokines are responsible for the modulation of immunological and inflammatory processes as well as proliferative responses and apoptosis. It has been recently suggested that such cytokines as interleukin 6 (IL-6), soluble interleukin 6 receptor (sIL-6R) and anti-inflammatory cytokines such as interleukin 10 (IL-10) may play a significant role in the pathogenesis of acute coronary syndromes.

Aim: To assess serum concentration of IL-6, sIL-6R and IL-10 in patients with ischaemic heart disease or acute myocardial infarction (AMI).

[Some adhesion molecules in blood of patients with chronic B-cell lymphocytic leukemia].

Adhesion molecules actively participate in all stages of leukocyte migration, directly or indirectly by means of appropriate ligands. Therefore the aim of study was the determination of concentrations of L-selectin and ICAM-1 in cell culture supernatante, broken lymphocyte and plasma. The measurement of cell adhesion molecules (CA) concentrations were performed by means of immunoenzymatic kits of ELISA type in the patients with chronic lymphocytic leukaemia in the clinicals stages I and III.

[Diagnostic value of some cytokines levels in chronic myelogenous leukemia].

Some cytokines play an important role in aetiology and pathogenesis of leukaemia. Majority of data available in the literature relates to a single cytokine in the cell culture in vitro. In patients with chronic myelogenous leukaemia several different cytokines are probably active.

[An attempt to clarify the etiopathogenesis of chronic lymphatic B-cell leukemia].

The paper presents current opinions about aetiology and pathogenesis of chronic lymphatic B-cell leukaemia.

[The role of some cytokines in the etiopathogenesis of leukemia].

Some cytokines play important role in etiology and pathogenesis of leukaemia. Most of them more stimulate than inhibit the proliferation of leukaemia cells.

[Cytokines in acute myeloid leukemia].

The cytokines belong to the group of main factors regulating of leukaemia cell proliferation. Most of information comprised in the literature concern the behaviour of single cytokines in the cell culture in vitro. In the organism of leukaemia patient many different cytokines, adhesion molecules, growth factors and other substances probably act in the same time.

[Cytokines in certain proliferative diseases of the hematopoietic system].

Some cytokines playing a great role in the proliferation of neoplastic haematopoietic system cells were described. Most of cytokines stimulate the cell proliferation in non-lymphoblastic leukemia, less of them act in lymphocytic leukemia and least in the other proliferative diseases of haematopoietic system.

[The role of some cytokines in the physiology of proliferation of hematopoietic cells].

Cytokines are of great importance in the regulation of proliferation of haematopoietic system cells. Most of known cytokines stimulate the proliferation of cells of granulocyte-macrophage, monocyte, and lymphocyte system. Some of them stimulate the proliferation of cells from erythrocyte, eosinophil and megakaryocyte systems and proliferation of B and T lymphocytes.