Publications by authors named "Kieron Walsh"
High cell density perfusion process of antibody producing CHO cells was developed in disposable WAVE Bioreactor™ using external hollow fiber filter as cell separation device. Both "classical" tangential flow filtration (TFF) and alternating tangential flow system (ATF) equipment were used and compared. Consistency of both TFF- and ATF-based cultures was shown at 20-35 × 10(6) cells/mL density stabilized by cell bleeds.
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- Developed a high cell density perfusion process for monoclonal antibody (MAb) production using disposable WAVE Bioreactor and external hollow fiber filters.
- Compared tangential flow filtration (TFF) and alternating tangential flow (ATF) systems, achieving cell densities above 10^8 cells/mL with comparable MAb production rates.
- Demonstrated effective cell cryopreservation and successful resuscitation, confirming the system's reliability for cell bank manufacturing.