The encapsulation of flavourzyme in nanoliposome by heating method.

The main objective of this study was to use heating method (HM) to prepare liposome without employing any chemical solvent or detergent. Plackett-Burman design (PBD) was applied for the screening of significant process variables including the lecithin proportion, the cholesterol/lecithin ratio, the pH of solution for liposome preparation, the enzyme/lecithin ratio, the stirring time, the process temperature, the speed of stirrer, the ratio of stirrer to the tank diameter, the application of homogenization, the method of adding enzyme and centrifugation conditions on the encapsulation efficiency (EE %) of liposome and the activity of liposomal Flavourzyme (LAPU(-1)) (P < 0.05).

Preparation and characterization of alginate and alginate-resistant starch microparticles containing nisin.

Delivery systems with sustained release of nisin have been proposed to improve stability and long-term effectiveness of this bacteriocin in foods. In this study, nisin was encapsulated in alginate (Alg) and alginate-resistant starch (Alg-RS) microparticles and its release was investigated. Studies found that the nisin concentration has significant influence on encapsulation efficiency (EE), loading capacity (LC) and size of both microparticles.

Incorporation of essential oil in alginate microparticles by multiple emulsion/ionic gelation process.

In this study, an o/w/o multiple emulsion/ionic gelation method was developed for production of alginate microparticles loaded with Satureja hortensis essential oil (SEO). It was found that the essential oil concentration has significant influence on encapsulation efficiency (EE), loading capacity (LC) and size of microparticles. The values of EE, LC and particle mean diameter were about 52-66%, 20-26%, and 47-117 μm, respectively, when the initial SEO content was 1-3% (v/v) .

Effect of process variables on supercritical fluid disruption of Ralstonia eutropha cells for poly(R-hydroxybutyrate) recovery.

This research focuses on the disruption of the gram-negative bacterium Ralstonia eutropha cells by supercritical CO2 for poly(R-hydroxybutyrate) (PHB) recovery. The variables affecting cell disruption such as drying strategy, type of modifier, and cultivation time, as well as operating pressure, temperature, and repeated release of supercritical CO2 pressure, have been studied. Effect of this disruption technique on PHB molecular mass was also investigated.