Publications by authors named "Kian Leong Tan"
The ultracentrifugation-based process is considered the common method for small extracellular vesicles (sEVs) isolation. However, the yield from this isolation method is relatively lower, and these methods are inefficient in separating sEV subtypes. This study demonstrates a simple benchtop filtration method to isolate human umbilical cord-derived MSC small extracellular vesicles (hUC-MSC-sEVs), successfully separated by ultrafiltration from the conditioned medium of hUC-MSCs.
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- Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles (hUC-MSCs-sEVs) have shown potential in treating diabetes, but previous dosages used in animal studies were too high for human application.
- This study evaluated the effects of lower doses of hUC-MSCs-sEVs on human skeletal muscle cells and type 2 diabetes mellitus (T2DM) rats, finding no significant cytotoxicity at 100 μg/mL and an 80-90% increase in glucose uptake at 20 μg/mL.
- The treatment improved glucose tolerance and had protective effects on blood count, while also demonstrating no significant harm to liver and kidney functions, indicating that hUC-M
Background: Copper complex has been gaining much attention in anticancer research as a targeted agent since cancer cells uptake more copper than non-cancerous cells. Our group synthesised a ternary copper complex, which is composed of 1,10-phenanthroline and tyrosine [Cu(phen)(L-tyr)Cl].3H0.
View Article and Find Full Text PDFThe objective of this study is to develop a simple protocol to isolate and characterise small extracellular vesicles (sEVs) from human umbilical cord-derived MSCs (hUC-MSCs). hUC-MSCs were characterised through analysis of morphology, immunophenotyping and multidifferentiation ability. SEVs were successfully isolated by ultrafiltration from the conditioned medium of hUC-MSCs.
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