ToTE: A global database on trees of the treeline ecotone.

Globally, treelines form a transition zone between tree-dominated forest downslope and treeless alpine vegetation upslope. Treelines represent the highest boundary of "tree" life form in high-elevation mountains and at high latitudes. Recently, treelines have been shifting upslope in response to climate warming, so it has become important to understand global tree diversity and treeline distributions.

Spatial variability in herbaceous plant phenology is mostly explained by variability in temperature but also by photoperiod and functional traits.

Whereas temporal variability of plant phenology in response to climate change has already been well studied, the spatial variability of phenology is not well understood. Given that phenological shifts may affect biotic interactions, there is a need to investigate how the variability in environmental factors relates to the spatial variability in herbaceous species' phenology by at the same time considering their functional traits to predict their general and species-specific responses to future climate change. In this project, we analysed phenology records of 148 herbaceous species, which were observed for a single year by the PhenObs network in 15 botanical gardens.

Functional trait diversity and aboveground biomass of herbaceous vegetation in temperate forests of Kashmir Himalaya.

Studying functional trait diversity can provide crucial clues about the adaptive survival strategies of regional plant species pool. Despite large-scale trait datasets available worldwide, the plant trait data from many biodiversity hotpot regions, like the Himalaya is still scarce. In this study, we aimed to investigate the plant functional traits and aboveground biomass of understory herbaceous vegetation in temperate forests of Overa-Aru wildlife sanctuary of Kashmir Himalaya.

Plant phenology shifts under climate warming: a systematic review of recent scientific literature.

Climate warming-driven temporal shifts in phenology are widely recognised as the foremost footprint of global environmental change. In this regard, concerted research efforts are being made worldwide to monitor and assess the plant phenological responses to climate warming across species, ecosystems and seasons. Here, we present a global synthesis of the recent scientific literature to assess the progress made in this area of research.

Different sets of traits determine transition of alien species along the invasion continuum.

Invasive alien species are currently considered as one of the dominant drivers of global environmental change. Till now, the majority of studies have focused on single or a few traits of alien species that facilitate their invasion. Also inclusion of all the traits which determine the transition of aliens along the different stages of invasion continuum (casual, naturalised and invasive) has remained largely overlooked.

Leaf functional traits vary among growth forms and vegetation zones in the Himalaya.

Compression of life zones along elevational gradients in mountains supports diverse vegetation types, and therefore offers ideal setting to study plant functional traits. Functional traits, the features that enable plants to live in varied environmental conditions, help in understanding ecological interactions, evolutionary adaptations, and predicting plant response to global change drivers. To date, little is known how the trait diversity varies across different growth forms and vegetation zones in mountains.

Diversity and utilization patterns of fodder resources in a Himalayan protected area.

In the Himalaya, collection of fodder from protected areas by local communities for sustenance of their livestock is an integral part of mountain farming systems. Here, we assess the diversity and utilization patterns of fodder resources collected by local communities from Overa-Aru wildlife sanctuary in Kashmir Himalaya. A multi-stage random sampling was employed to select sample villages (5) and households (81) for the survey.

Sustaining Traditional Ethnomedicinal Knowledge and Protected Areas in Synergy: A Case Study of Overa-Aru Wildlife Sanctuary in Kashmir Himalaya.

The ethnic communities living near protected areas are critically dependent on medicinal plants for their healthcare. Although several ethnomedicinal studies are available from the Himalaya, quantitative studies focusing on protected areas in this region are largely overlooked. In this study, we aimed to document the ethnomedicinal plants of the Overa-Aru wildlife sanctuary in Kashmir Himalaya.

Plant invasion shifts soil microbiome and physico-chemical attributes along an elevational gradient in Kashmir Himalaya.

Soil microbial communities, being situated at the interface of aboveground plant and belowground soil systems, can play a pivotal role in determining ecosystem response to the drivers of global environmental change, including invasive species. In mountains, invasive plants occurring along elevational gradients offer a unique natural experimental system to investigate the impact of invasions in determining patterns and relationships of soil microbial diversity and nutrient pools at much shorter spatial distances. Here, we studied the impact of a global plant invader, Leucanthemum vulgare, on the diversity of soil microbiome and physico-chemical attributes along an elevational gradient (1760-2880 m) in Kashmir Himalaya.

From forest floor to tree top: Partitioning of biomass and carbon stock in multiple strata of forest vegetation in Western Himalaya.

The foremost role of forest vegetation in storing biomass and carbon (C) stock constitutes one of the main nature-based solutions to mitigate climate change. In this study, we aimed to quantify biomass and C stock partitioning in multiple vegetation strata (tree, shrub, herb, and ground floor layers) of major forest types in Jammu and Kashmir, Western Himalaya, India. We used a stratified random cluster sampling strategy to collect field data in 96 forest stands of 12 forest types (altitudinal range: 350 to 3450 m) in the study region.

Alien flora causes biotic homogenization in the biodiversity hotspot regions of India.

Biotic homogenization by invasive alien species is one of the dominant drivers of global environmental change. However, little is known about the patterns of biotic homogenization in global biodiversity hotspots. Here, we fill this knowledge gap by studying the patterns of biotic homogenization and associated geographic and climatic correlates in Indian Himalayan Region (IHR).

Unravelling diversity, drivers, and indicators of soil microbiome of Trillium govanianum, an endangered plant species of the Himalaya.

In an era of global environmental change, conservation of threatened biodiversity and ecosystem restoration are formidable ecological challenges. The forest understory strata and the belowground soil environment including rhizospheric microbial communities, which are crucial for ecosystem functioning and overall forest biodiversity maintenance, have remained understudied. Here, we investigate the soil microbiome of Trillium govanianum - an endangered Himalayan Forest herb, to unravel the underground diversity, drivers, and potential indicators of the microbial community.

Simultaneous estimation of paclitaxel and erlotinib in plasma by liquid chromatography/(+) electrospray tandem mass spectrometry: application in formulation development and pharmacokinetics.

The bio-analytical method was developed and validated for simultaneous detection and quantification of paclitaxel (PAC) and erlotinib (ERL) in plasma samples. The sample preparation process was accomplished by liquid-liquid extraction technique. The dried and reconstituted samples were subjected to chromatography on Discovery -C18 (50 × 4.

Climate warming-driven phenological shifts are species-specific in woody plants: evidence from twig experiment in Kashmir Himalaya.

Experimental evidences in support of climate warming-driven phenological shifts are still scarce, particularly from the developing world. Here, we investigated the effect of experimental warming on flowering phenology of selected woody plants in Kashmir Himalaya. We selected the twigs of four congeneric pairs of temperate woody species (Prunus, Populus, Ulmus, Viburnum)-typical spring-flowering plants in the region.

Multiple-reaction monitoring (MRM) LC-MS/MS quantitation of venlafaxine and its O-desmethyl metabolite for a preclinical pharmacokinetic study in rabbits.

Preclinical pharmacokinetic (PK) studies in animal models during the formulation development phase give preliminary evidence and near clear picture of the PK behavior of drug and/or its dosage forms before clinical studies on humans and help in the tailoring of the dosage form according to the expected and requisite clinical behavior. The present work reports a first of its kind preclinical PK study on extended-release (ER) solid oral dosage forms of venlafaxine (VEN) in New Zealand White rabbits. The VEN is a highly prescribed and one of the safest and most effective therapeutic agents used in the treatment of different types of depression disorders worldwide.

Patterns of floristic and functional diversity in two treeline ecotone sites of Kashmir Himalaya.

Globally, the treelines at higher elevations in mountains are reported to be advancing up-slope in response to recent climate warming. However, little is known about the treeline advancement in the Himalaya due to paucity of baseline vegetation data with which to compare, thus making their assessment and monitoring challenging. To fill this knowledge gap, the present study documented floristic and functional diversity of two treeline ecotone sites in Kashmir Himalaya.

Development of a bioanalytical method for the quantification of sinococuline in human plasma and its application in phase I clinical pharmacokinetic study.

A selective, highly sensitive, precise, and novel bioanalytical method has been developed and validated to quantify sinococuline, an active constituent present in the phytopharmaceutical drug product containing Cocculus hirsutus plant extract, in vivo. Chromatographic separation was achieved on a Luna Omega Polar-C18 bonded analytical column maintained at 45°C. The isocratic mobile phase consisted of methanol and ammonium formate buffer (60:40, v/v) at acidic pH with a low flow rate of 0.

Substantial shifts in flowering phenology of Sternbergia vernalis in the Himalaya: Supplementing decadal field records with historical and experimental evidences.

In an age of anthropocene, shifting plant phenology is one of the most striking biological indicators of global environmental change. Majority of the studies reporting shifts in plant phenology are available from the North America and Europe and largely scarce from the developing world, including the Himalaya; and studies integrating multiple methodological approaches to investigate the climate-driven phenological shifts are too rare. Here, we report the shifts in spring flowering phenology of model plant species, Sternbergia vernalis in response to the changing climate in Kashmir Himalaya, by integrating decadal field observational records with long-term herbarium and dated-photograph data, and supported with experimental evidences.

Predicting shifts in distribution range and niche breadth of plant species in contrasting arid environments under climate change.

Arid environments face extreme risk from contemporary climate change; therefore, predicting the shifts in species distribution range and niche breadth in these environments assumes urgent research priority. Here we report the potential distribution and predict future distribution range of two model plant species typically representing contrasting environments across Asia and Africa: hot-arid Ephedra foliata and cold-arid E. gerardiana.

Arterolane-piperaquine-mefloquine versus arterolane-piperaquine and artemether-lumefantrine in the treatment of uncomplicated Plasmodium falciparum malaria in Kenyan children: a single-centre, open-label, randomised, non-inferiority trial.

Background: Triple antimalarial combination therapies combine potent and rapidly cleared artemisinins or related synthetic ozonides, such as arterolane, with two, more slowly eliminated partner drugs to reduce the risk of resistance. We aimed to assess the safety, tolerability, and efficacy of arterolane-piperaquine-mefloquine versus arterolane-piperaquine and artemether-lumefantrine for the treatment of uncomplicated falciparum malaria in Kenyan children.

Methods: In this single-centre, open-label, randomised, non-inferiority trial done in Kilifi County Hospital, Kilifi, coastal Kenya, children with uncomplicated Plasmodium falciparum malaria were recruited.

Early Evidence of Shifts in Alpine Summit Vegetation: A Case Study From Kashmir Himalaya.

Under the contemporary climate change, the Himalaya is reported to be warming at a much higher rate than the global average. However, little is known about the alpine vegetation responses to recent climate change in the rapidly warming Himalaya. Here we studied vegetation dynamics on alpine summits in Kashmir Himalaya in relation to measured microclimate.

Plant invasion alters the physico-chemical dynamics of soil system: insights from invasive Leucanthemum vulgare in the Indian Himalaya.

Understanding the impact of plant invasions on the terrestrial ecosystems, particularly below-ground soil system dynamics can be vital for successful management and restoration of invaded landscapes. Here, we report the impacts of a global plant invader, Leucanthemum vulgare Lam. (ox-eye daisy), on the key physico-chemical soil properties across four sites selected along an altitudinal gradient (1600-2550 m) in Kashmir Himalaya, India.