Passive acoustic monitoring for seabed bubble flows: Case of shallow methane seeps at Laspi Bay (Black Sea).

This research quantifies the gas release rate from a natural shallow methane seep site in the Laspi Bay (Black Sea), whose origin is thermocatalytic. An adaptive single bubble identification technique was applied to analyze gas volume and release rates from passive acoustic data. Gas from the seafloor was emitted by single bubbles that occurred in clusters.

Shift in hemocyte immune parameters of marine bivalve Mytilus galloprovincialis (Lamarck, 1819) after exposure to methane.

Methane are widely used in industry as an emerge source may be released significantly higher aquatic ecosystems due to gas seepages. In this study, short-term (90 min) methane effects on bivalve hemocytes were investigated using flow cytometry. Hemocyte parameters including hemolymph cellular composition, phagocytosis activity, mitochondrial membrane potential and reactive oxygen species (ROS) content were evaluated in the mussel Mytilus galloprovincialis (Lamarck, 1819) exposed to hypoxia (control group), pure methane and industrial methane (industrial hydrocarbon mixture).