Combined autoradiographic and cytophotometric study of proliferating cell populations in human gliomas with description of a logarithmic screen method of cytophotometry.

Cell populations of 19 human brain tumors were studied by the combination of autoradiographic and cytophotometric methods. In some tumors there were up to 20% polyploid cells, but in the majority of cases the number of labeled polyploid cells was minimal. In 17 cases, labeled cells were within the range 2c to 4c or 1c to 3c: i.

Circadian oscillations of nucleic acid and protein content in functionally different neuron-neuroglia units of rat central nervous system.

Scanning integrating visible cytospectrophotometry of gallocyanin-, amido black-, fast green- or ammoniacal silver-stained sections of various areas of the central nervous system of adult rats sacrificed at 4 h intervals has demonstrated the presence of circadian changes in RNA, total protein and total histone content as well as in arginine-rich to lysine-rich histone ratio in the neurons and in their perineuronal satellite neuroglia. The peak of the content per cell of the macromolecular components studied was in some cases elevated above their lowest level up to 1.7-fold.