Sol-gel synthesis, structure, and dielectric properties of LaLiTiAlO solid solutions.

LaLiTiAlO were synthesized using the sol-gel Pechini route. Ceramic samples were sintered in the temperature range of 1240-1300 °C in the air atmosphere. It was found that in the concentration range of 0.

[Ultrastructural characteristics of NKHI+- and CD8+-subpopulations of large granule-containing lymphocytes in the blood of patients with Hodgkin's disease].

Ultrastructure of NKH1+ and CD8+ subpopulations of large granular lymphocytes (LGL) obtained by the flow cytometry method using corresponding monoclonal antibodies was studied in patients with Hodgkin's disease. Electron microscopy has identified different changes in ultrastructure of LGL, especially of osmiophilic granules. The results indicate significant changes in the functional activity and defect of LGL in blood at early stages of the disease.

[The number and ultrastructure of large granule-containing lymphocytes (natural killer cells) in the blood of patients with diabetes mellitus type 1].

The content and ultrastructure of blood large granule containing lymphocytes (LGL) associated with the system of natural killer cells (NKC) were investigated in primary untreated patients with type I diabetes mellitus using light and electron microscopy. A marked decrease in the content of LGL and change in their submicroscopic structure indicating disorder of their function, were noted in the blood of patients with type I diabetes mellitus as compared to those in the blood of healthy subjects. LGL cytoplasm revealed a decrease in the number and size of specific electron dense granules (azurophil granules), their destruction and vacuolization were noted; the number of peculiar submicroscopic formations (parallel tubular structures) was on an increase.

[Ultrastructure of large granule-containing lymphocytes possessing natural killer activity].

By means of light and electron microscopy, ultrastructural cytochemistry and immune cytochemistry methods, contents and ultrastructure of large granule-containing lymphocytes (LGL) have been studied in human blood--this is cell population possessing natural killer and, partly, antibody-depending cytotoxicity. The LGL concentrates are isolated from blood applying successive physical-chemical methods, differential centrifugation in the density gradient of pack-phycoll and percoll included. Separate LGL populations are marked by means of rosette-forming reaction with sheep erythrocytes and monoclonal antibodies OKT4 and OKT8.

[Count and ultrastructure of large granule-containing lymphocytes in the blood of lymphogranulomatosis patients].

The blood-level and ultrastructure of large granular lymphocytes (LGL)--a subpopulation with natural killer activity--were studied in 120 cases of Hodgkin's disease and 50 healthy controls. A marked increase in the relative and total number of LGLs was observed in patients with complication--free stage I--II tumor. Electron microscopy identified changes in the size and ultrastructure of osmiophilic granules as well as a relatively higher level of parallel tubular arrays in LGL cytoplasm.

[Ultrastructure of human blood T-lymphocytes marked with monoclonal antibodies].

The ultrastructure of different human blood T-lymphocyte subpopulations was studied by immunoelectron microscopy using "Orthoclone" monoclonal antibodies (OKT-3, OKT-4 and OKT-8) and peroxidase-antiperoxidase (PAP) complex. It was shown that T-lymphocyte subpopulations, distinct in their surface markers and function, possessed some specific features of submicroscopic structure. OKT-4+ cells (helper/inductor) were characterized by small size, high nucleus/cytoplasm ratio and few cytoplasmic organelles.

[Comparative immunological and electron microscopic study of the structure of E-RFC and OKT-3+ lymphocytes from human blood].

Methods of immune electrone microscopy have revealed that OKT-3+ cells and E-RFC of human blood have a similar ultrastructure. Definite morphologic spectrum is observed within each of these fractions, that testifies to the heterogeneity of surface markers of circulating T-lymphocytes and their ultrastructure.

[Ultrastructure of hematopoietic and stromal cells of the subendosteal region of the bone marrow].

Using electron microscopic cytochemistry and immunoelectron microscopy, the ultrastructure of bone marrow (BM) cells of the subendosteal region with a high colony-forming (CFUs) ability was studied. In comparison with the central part of BM, the subendosteal region of CBA and BALB/c mice contains a higher number of lymphocyte-like mononuclears, bearing an antigen, common with the brain surface one but negative for peroxidase and acid and alkaline phosphatase. The ultrastructure of these cells is similar to that of presumptive hematopoietic stem cells.

[Electron microscopy and cytochemistry studies of lymphocyte subpopulations in corticosteroid deficiency].

The authors studied the number, structure and function of different blood T lymphocyte subpopulations in experimental hypocorticoidism of male CBA mice with the use of modern hematological, cytochemical, immunological and electron microscopy methods. One week after bilateral adrenalectomy the blood showed a considerable absolute and relative lymphocytosis determined by an increase in the content of Thy I-positive, ANAE-positive (giving positive reaction during identification of acid non-specific alpha-naphthylacetate esterase) cells and large granule-containing lymphocytes, a group, which includes natural killers and cells possessing antibody-dependent cell-mediated cytotoxicity. Transmission and scanning electron microscopy demonstrated changes in submicroscopic structure of different blood lymphocytes in adrenalectomized mice.

Immunoelectron microscopy of the bone marrow mononuclears labelling with rabbit anti-mouse brain serum using peroxidase-anti-peroxidase method.

Ultrastructural features of mouse bone marrow mononuclears (BMM) labelled with peroxidase-anti-peroxidase (PAP) complex using rabbit anti-mouse brain serum (RAMBS) and rabbit anti-mouse brain serum, absorbed with thymocytes (RAMBST) were investigated. After incubation of cells with RAMBST which have high activity against mouse pluripotent hemopoietic stem cells (CFUs) about 0.6% of BMM were labelled.

[Ultrastructure, metabolism and function of white blood cells during the development of an immune response and exposure to glucocorticoids. III. Effect of immunization on the number and ultrastructure of rabbit bone marrow cells].

The characteristic features of the early period after the immunization of rabbits with bovine serum albumin are the decreased content of ripe neutrophil forms, the increased numbers of eosinophils and basophils, as well as changes in the ultrastructure of granulocytes in the bone marrow. The destruction of the primary and secondary granules, vacuolization and an increase in acidic phosphatase activity can be observed in neutrophils. Each stage of immunogenesis has its characteristic ratio of various kinds of lymphocytes.

[Submicroscopic formations of an unknown nature in the eosinophils of the blood of dogs].

The authors describe ultrastructure in dog blood eosinophils, unknown formerly. These formations look like dumb-bells or rods, 0,003-0,005 micron in length. A double-contour membrane is distinct under high magnification.