Modification of isothermal test for detecting DNA of pathogens of tick-borne borreliosis.

Early diagnosis of tick-borne borreliosis determines the indications for etiotropic therapy, and the detection of borrelia in a tick that has bitten you serves as the basis for antibiotic prophylaxis. To determine the causative agent of borreliosis, PCR methods are most widely used, which requires special conditions for organizing the work of laboratories and the use of expensive equipment. In addition, the procedure for isolating bacterial DNA and subsequent amplification takes several hours of working time.

[Oxidative modification of human serum proteins. A method of determining it].

A rate of protein oxidative destruction may be estimated from the reaction of the resultant carbonyl derivatives of amino acids reaction with 2,4-dinitrophenyl hydrazine. The procedure for estimation of 2,4-dinitrophenyl hydrazones was modified for clinical application. The initiating effect of nonenzymatic components (Fe(3+)-ascorbic acid, Fe(2+)-O2, Fe(2+)-H2O2) was in the metal-catalyzing oxidation of proteins.

[The action of bilirubin in vitro on the antioxidant activity and lipid peroxidation of the plasma and erythrocytes of cord blood].

Plasma and erythrocytes of cord blood were incubated with bilirubin (40-400 mkMol/l) for 30 min. This incubation resulted in reduction of lipid peroxidation both in plasma and erythrocytes. The antioxidant activity increased in erythrocytes after incubation, but decreased in plasma.

[The results of the therapeutic monitoring of piracetam in parturients and newborn infants].

Overall 30 women in childbirth with preeclampsia and fetoplacental dysfunction received piracetam solution which was given initially in a dose of 5 g by intravenous drip, rapidly since the onset of labour and then every 2 hours till the end of labour in a dose of 2 g by jet injection. This made it possible to increase fetus resistance to hypoxic conditions. The concentrations of piracetam within the range of 60-80 g/l in the blood of women in childbirth should be viewed as minimum therapeutic.

Cerebral circulation control in healthy full-term neonates.

A study of the dynamic regulation of cerebral circulation and its metabolic regulation in hyperoxia in healthy mature infants has shown that dynamic regulation acquires some features that are typical of healthy adults by the second day of life. Hyperoxia on the first day after birth does not influence the volumetric parameters of cerebral circulation, but on subsequent days it results in their noticeable decrease. Thus, in healthy mature infants the development of dynamic regulation of cerebral circulation and its metabolic regulation occurs on the first day of life.