The aim of this study was to investigate the effects of retro-cavity preconditioning with or without 17% ethylenediaminetetraacetic acid (EDTA) solution on root surface pH as well as dislodgement resistance of NeoMTA2 and MTA Flow retro-fills. Forty-eight single-rooted human incisors were selected. After completion of endodontic treatment, root-end resections were performed, and retro-cavities were prepared.
View Article and Find Full Text PDFAntibacterial activity against endodontic pathogens is a desirable feature for root canal sealers. The objective of this study was to compare the antibacterial effect of three resin-based endodontic sealers (AH26, Adseal, and Beta RCS) against in vitro. The antibacterial properties of the sealers were assessed against using agar diffusion test (ADT) for fresh state (N=10) and direct contact test (DCT) for freshly-mixed and set states of the materials (N=10).
View Article and Find Full Text PDFBackground: Biocompatibility and induction of mineralized tissue formation are the properties expected from a material used in vital pulp therapy and repair of perforations. Cold ceramic (SJM, Iran; CC) is a newly introduced calcium silicate-based cement for above mentioned therapeutic applications. This in-vitro study aimed to compare the effect of CC and White MTA-Angelus (MTA) on cell viability, attachment, odontogenic differentiation, and calcification potential of human dental pulp stem cells (DPSCs) and periodontal ligament fibroblasts (PDLFs).
View Article and Find Full Text PDF. This study aimed to colorimetric comparison of internal bleaching with and without removing mineral trioxide aggregate (MTA) on induced coronal tooth discoloration by MTA cement. In this experimental study, twenty human teeth were prepared.
View Article and Find Full Text PDFBackground: This study aimed to assess the effect of ultrasonic activation of photosensitizer on the efficacy of Antimicrobial Photodynamic Therapy (aPDT) against Enterococcus faecalis and penetration depth of photosensitizer.
Materials And Methods: In this ex vivo study, mature microbial biofilm of E. faecalis was formed in the root canals of 58 single-rooted single-canal mandibular incisors following their decoronation.
Objectives: The aim of this study was to assess the antibacterial activities of OrthoMTA, RetroMTA, and ProRoot MTA against (), (), and ()
Materials And Methods: Each material was mixed on a glass slab using a spatula and was placed in columns containing the filter membrane of the modified membrane-enclosed immersion test (MEIT) system. The materials were sterilized after setting. The columns containing the sterilized test materials were placed in microcentrifuge tubes containing 500 μl of bacterial suspension.
Introduction: Reducing the bacterial count from the root canal system is one of the main stages in root canal treatment. The aim of the present clinical study was to compare the antibacterial effect of four intracanal irrigants in primary endodontic infections using both microbiological culture and quantitative Real-time Polymerase Chain Reaction (qRT-PCR) technique.
Methods And Materials: Forty patients with primarily infected single rooted premolars were selected and then randomly divided into 4 groups according to the intra canal irrigant used: 5.
Objectives: The aim of this study was to compare apical sealing ability of alpha and beta phases of gutta-percha by means of bacterial leakage model.
Materials And Methods: Fifty single-rooted human premolars were selected. The root canals were prepared with Mtwo rotary instruments up to apical #35.
Introduction: The aim of this in vitro study was to compare the efficacy of ProTaper retreatment (ProTaper R) and Mtwo retreatment (Mtwo R) files in removing gutta-percha and GuttaFlow from endodontically treated straight root canals.
Methods And Materials: The root canals of 60 human mandibular single-rooted premolars were prepared and randomly divided into two groups (n=30). In groups A and B the root canals were obturated using lateral condensation of gutta-percha plus AH 26 and GuttaFlow, respectively.
Objectives: This study aimed to assess the radicular wall thickness in mandibular incisors with two canals and find the maximum and minimum thickness to prevent root canal treatment (RCT) procedural errors.
Materials And Methods: A total of 160 extracted mandibular incisors were selected and radiographed; out of which, 55 had two canals. Three parallel transverse sections were made in each tooth at 1mm below the cementoenamel junction (CEJ), mid-root and 1 millimeter to the apex.
Objectives: This study aimed to assess the effect of chloroform, eucalyptol and orange oil solvents on the microhardness of human root dentin.
Materials And Methods: Sixty-eight single-rooted single-canal extracted human premolar teeth were used. Tooth crowns were separated from the roots at the cementoenamel junction (CEJ).
Objectives: This study was performed to evaluate the cytotoxicity of four calcium silicate-based endodontic cements at different storage times after mixing.
Materials And Methods: Capillary tubes were filled with Biodentine (Septodont), Calcium Enriched Mixture (CEM cement, BioniqueDent), Tech Biosealer Endo (Tech Biosealer) and ProRoot MTA (Dentsply Tulsa Dental). Empty tubes and tubes containing Dycal were used as negative and positive control groups respectively.
Objectives: To compare the mechanical properties of Resilon and gutta-percha cones after storing at three different temperatures.
Materials And Methods: One-hundred standardized size 40/02 gutta-percha cones and 100 Resilon cones were randomly divided into four groups. The first group of the materials were tested immediately after receipt as the control group.
Objective: Handling of endodontic sealers is greatly dependent on their elasticity and flow ability. We compared the viscoelastic properties of three root canal sealers.
Materials And Methods: AH Plus (Dentsply, De Trey, Konstanz, Germany), Endofill (Dentsply Hero, Petrópolis, Rio de Janeiro, Brazil) and AH26 (Dentsply, De Trey, Konstanz, Germany) were mixed according to the manufacturers' instructions.
Aim: To compare the accuracy of digital radiography (DR), multidetector computed tomography (MDCT) and cone beam computed tomography (CBCT) in detecting vertical root fractures (VRF) in the absence and presence of gutta-percha root filling.
Methodology: The root canals of 100 extracted human single-rooted teeth were prepared and randomly divided into four groups: two experimental groups with artificially fractured root and two intact groups as controls. In one experimental and one control group, a size 40, 0.
Background And Objective: Enamel matrix proteins are involved in the development and regeneration of root cementum and in its attachment to dentin; however, the mechanisms through which this occurs have yet to be elucidated. The present study was therefore carried out to evaluate the mitogenic and proliferative responses of human periodontal fibroblast (HPLF) cells to Emdogain (EMD), and the potential role of cyclooxygenase 2 (COX-2) in this process.
Material And Methods: We investigated the effects of EMD on 5-bromo-2'-deoxyuridine (BrdU) incorporation, colchicine freezing of mitosis, XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] reduction and Trypan Blue dye exclusion, with or without celecoxibe, a selective cyclooxygenase-2 (COX-2) inhibitor; we also evaluated the expression of COX-2 mRNA and COX-2 protein in response to EMD.
Introduction: There is some controversy about the effect of enamel matrix derivative (EMD) on inflammation and resorption. The aim of this study was to investigate the effect of EMD on the inflammatory response of monocytes and their phagocytic activity in vitro.
Methods: Human monocytes were incubated in complete medium (CM) and exposed to 50, 100, and 200 microg/mL EMD for different time points (12, 24, 48, and 72 hours).
Introduction: Successful endodontic treatment is dependent on a perfect knowledge of normal root canal anatomy and variations from the norm. This study was aimed to investigate the root canal anatomy of mandibular first premolars by both radiography and cross-sectional methods in an Iranian population.
Methods: Two hundred seventeen extracted human mandibular first premolars were used.
Introduction: It has been demonstrated that organic content of the root canals can influence the antimicrobial capability of chemical irrigants. The aim of this study was to evaluate the effect of bovine serum albumin (BSA), as an organic material, on the antimicrobial activity of several intracanal irrigants.
Materials And Methods: Bactericidal activity of Ethylenediaminetetraacetic acid (EDTA) 17%, citric acid 10%, Sodium hypochlorite (NaOCl) 5.
The purpose of this study was to compare the efficacy of SmearClear (Sybron Endo, Orange, CA), 17% EDTA, and 10% citric acid in smear layer removal. Forty-eight extracted single-rooted human teeth were randomly divided into 4 groups (n = 12) and instrumented using Mtwo nickel-titanium rotary instruments. Each canal was subsequently irrigated with one of the following solutions: 5.
View Article and Find Full Text PDFIntroduction: The aim of this study was to compare the cleaning efficacy of three irrigants used during and after instrumentation.
Materials And Methods: Eighty-four single rooted human teeth were randomly divided into 7 groups, 12 cases each, and canals were instrumented with Mtwo rotary systems. 5.
Introduction: Recently, attention has been drawn to the influence of smear layer removal on apical seal, and the relation of root canal filling material with canal wall surface in the presence and absence of the smear layer. The purpose of this study was to evaluate the influence of the smear layer removal on the apical sealing ability of AH26 sealer.
Materials And Methods: Forty extracted human anterior teeth were used in this study.