Impact of Silver Nanoparticles on Gene Expression in Producer Aflatoxin B1.

Aim: In this study, we evaluated the effect of silver nanoparticles (AgNPs) on the production of aflatoxin B (AFB) through assessment the transcription activity of aflatoxin biosynthesis pathway genes in ATCC28542.

Material And Methods: The mRNAs were quantitative by Real Time-polymerase chain reaction (qRT-PCR) of grown in yeast extract sucrose (YES) medium containing AgNPs. Specific primers that are involved in the AFB biosynthesis which highly specific to , O-methyltransferase gene (), were designed and used to detect the fungus activity by quantitative PCR assay.