One hallmark of cancer is the upregulation and dependency on glucose metabolism to fuel macromolecule biosynthesis and rapid proliferation. Despite significant pre-clinical effort to exploit this pathway, additional mechanistic insights are necessary to prioritize the diversity of metabolic adaptations upon acute loss of glucose metabolism. Here, we investigated a potent small molecule inhibitor to Class I glucose transporters, KL-11743, using glycolytic leukemia cell lines and patient-based model systems.
View Article and Find Full Text PDFThe incorporation of mitochondria into early eukaryotes established organelle-based biochemistry and enabled metazoan development. Diverse mitochondrial biochemistry is essential for life, and its homeostatic control via mitochondrial dynamics supports organelle quality and function. Mitochondrial crosstalk with numerous regulated cell death (RCD) pathways controls the decision to die.
View Article and Find Full Text PDFUrothelial carcinoma (UC) is the most common malignancy of urinary bladder. It is the 9th leading cause of death worldwide and second most common genitourinary malignancy among male. Incidence is increasing in developing countries like Bangladesh.
View Article and Find Full Text PDFSpherically shaped trimetallic MnAlO (MAO) nanoadsorbent was prepared in an one-pot synthesis process for the removal of excess fluoride from water. The adsorbent was characterized by thermogravimetric analysis (TGA), X-ray diffraction study (XRD), Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), etc. The adsorption property for fluoride on the MAO was analyzed by batch experiments varying the adsorbent dose, pH, contact time, and initial fluoride concentration.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
December 2023
A 3D flower-shaped bimetallic nanocomposite zirconium magnesium oxide (ZMO) was prepared first time by the controlled solution combustion method using triethanolamine (TEA) as a fuel and chelating agent. The composite material was used to remove excess fluoride via adsorption. The thermal stability of the adsorbent was characterized by thermogravimetric analysis (TGA).
View Article and Find Full Text PDFUnlabelled: T-cell acute lymphoblastic leukemia (T-ALL) is a NOTCH1-driven disease in need of novel therapies. Here, we identify a NOTCH1-SIRT1-KAT7 link as a therapeutic vulnerability in T-ALL, in which the histone deacetylase SIRT1 is overexpressed downstream of a NOTCH1-bound enhancer. SIRT1 loss impaired leukemia generation, whereas SIRT1 overexpression accelerated leukemia and conferred resistance to NOTCH1 inhibition in a deacetylase-dependent manner.
View Article and Find Full Text PDFA novel porous spherical-shaped magnesium zinc binary oxide (MZO) was successfully prepared for the first time using a chemical process for fluoride removal and photocatalytic methylene blue (MB) and Congo red (CR) dye degradation. XRD, FESEM, and TEM were studied for phase formation, topographic, crystallographic, and detailed structural information. The surface charge and optical properties of the adsorbent were studied by zeta potential and photoluminescence spectra.
View Article and Find Full Text PDFIn this growing age of population,agriculture plays a significant role by providing food and employment to millions of people. But to meet the growing need of food day by day the demand of fast and quality plant production becomes a must. Fertilization is one of such activities which are people accustomed to do for this purpose from a very long time.
View Article and Find Full Text PDFOkra () is a traditionally important vegetable herb used to treat numerous illnesses, including diabetes mellitus, in many rural parts of Bangladesh and the South Asian subcontinent. However, the scientific evidence for the aforementioned properties has not been adequately validated. Hence, the aim of this study was to explore the antidiabetic, antilipidemic activity of okra mucilage powder, and to compare its effectiveness with the isolated peel-seed of okra after mucilage extraction in Swiss albino mice.
View Article and Find Full Text PDFThis study set out to examine the combined effects of the supplementation of a dietary palm oil (PO) and sunflower oil (SO) blend, 0. 25% L-Arginine (L-Arg), and different levels of vitamin E (Vit E) on growth performance, fat deposition, cytokine expression, and immune response in broilers. A total of 216 1-day-old male broiler chicks (Cobb500) were randomly distributed into six dietary groups as follows: Diet 1: 6% palm oil (negative control); Diet 2: PO and SO blend (4% palm oil and 2% sunflower oil) + 0.
View Article and Find Full Text PDFThe trimetallic Fe/Al/Ti (1:1:1) nanocomposite (FAT), synthesized by an adaptable tuned chemical route, offers a new approach for water treatment, for example, the de-fluoridation and photodegradation soluble dye methylene blue (MB) at pH 7. FAT acted as a good fluoride scavenger in the presence of other co-ions and within a widespread pH range (pH 2-11). The photodegradation efficiencies were >90% for different concentrations of MB solutions.
View Article and Find Full Text PDFRtg1p and Rtg3p are two basic helix-loop-helix, retrograde transcription factors in the budding yeast Both factors heterodimerize to activate the transcription of nuclear genes in response to mitochondrial dysfunction and glutamate auxotrophy, but are not well characterized in other yeasts. Here, we demonstrate that the Rtg1p/Rtg3p-mediated retrograde signaling pathway is absent in the methylotrophic yeast We observed that Rtg1p (PpRtg1p) heterodimerizes with Rtg3p and functions as a nuclear, retrograde transcription factor in , but not in We noted that Rtg3p lacks a functional leucine zipper and interacts with neither Rtg1p (ScRtg1p) nor PpRtg1p. In the absence of an interaction with Rtg3p, PpRtg1p has apparently acquired a novel function as a cytosolic regulator of multiple metabolic pathways, including biosynthesis of glutamate dehydrogenase 2 and phosphoenolpyruvate carboxykinase required for the utilization of glutamate as the sole carbon source.
View Article and Find Full Text PDFThe present study assessed the effect of feeding palm oil (PO), sunflower oil (SO) and their combination on performance, fat deposition, fatty acid composition and lipogenic gene expression of broilers reared for 42 days. A total of 144 1-day-old broilers (Cobb500) were randomly allotted into four treatment diets with each having six replicates of six chicks in each replicate following a completely randomized design. Live weight gain and feed efficiency was significantly (P < 0.
View Article and Find Full Text PDFBackground: The visceral leishmaniasis (VL) elimination program in Bangladesh is in its attack phase. The primary goal of this phase is to decrease the burden of VL as much as possible. Active case detection (ACD) by the fever camp method and an approach using past VL cases in the last 6-12 months have been found useful for detection of VL patients in the community.
View Article and Find Full Text PDFThe alarming rise of ciprofloxacin-resistant Pseudomonas aeruginosa has been reported in several clinical studies. Though the mutation of resistance genes and their role in drug resistance has been researched, the process by which the bacterium acquires high-level resistance is still not well understood. How does the genomic evolution of P.
View Article and Find Full Text PDFBackground: Proteogenomic mapping is an approach that uses mass spectrometry data from proteins to directly map protein-coding genes and could aid in locating translational regions in the human genome. In concert with the ENcyclopedia of DNA Elements (ENCODE) project, we applied proteogenomic mapping to produce proteogenomic tracks for the UCSC Genome Browser, to explore which putative translational regions may be missing from the human genome.
Results: We generated ~1 million high-resolution tandem mass (MS/MS) spectra for Tier 1 ENCODE cell lines K562 and GM12878 and mapped them against the UCSC hg19 human genome, and the GENCODE V7 annotated protein and transcript sets.
Membrane proteomics, the large-scale analysis of membrane proteins, is often constrained by the difficulties of achieving fully resolvable separation and resistance to proteolysis, both of which could lead to low recovery and low identification rates of membrane proteins. Here, we introduce a novel integrated approach, GELFrEE Optimized FASP Technology (GOFAST) for large-scale and comprehensive membrane proteins analysis. Using an array of sample preparation techniques including gel-eluted liquid fraction entrapment electrophoresis (GELFrEE), filter-aided sample preparation (FASP), and microwave-assisted on-filter enzymatic digestion, we identified 2 090 proteins from the membrane fraction of a leukemia cell line (K562).
View Article and Find Full Text PDFData from the Encyclopedia of DNA Elements (ENCODE) project show over 9640 human genome loci classified as long noncoding RNAs (lncRNAs), yet only ~100 have been deeply characterized to determine their role in the cell. To measure the protein-coding output from these RNAs, we jointly analyzed two recent data sets produced in the ENCODE project: tandem mass spectrometry (MS/MS) data mapping expressed peptides to their encoding genomic loci, and RNA-seq data generated by ENCODE in long polyA+ and polyA- fractions in the cell lines K562 and GM12878. We used the machine-learning algorithm RuleFit3 to regress the peptide data against RNA expression data.
View Article and Find Full Text PDFEukaryotic cells make many types of primary and processed RNAs that are found either in specific subcellular compartments or throughout the cells. A complete catalogue of these RNAs is not yet available and their characteristic subcellular localizations are also poorly understood. Because RNA represents the direct output of the genetic information encoded by genomes and a significant proportion of a cell's regulatory capabilities are focused on its synthesis, processing, transport, modification and translation, the generation of such a catalogue is crucial for understanding genome function.
View Article and Find Full Text PDFMicrobes have developed resistance to nearly every antibiotic, yet the steps leading to drug resistance remain unclear. Here we report a multistage process by which Pseudomonas aeruginosa acquires drug resistance following exposure to ciprofloxacin at levels ranging from 0.5× to 8× the initial MIC.
View Article and Find Full Text PDFMotivation: The identification of peptides by tandem mass spectrometry (MS/MS) is a central method of proteomics research, but due to the complexity of MS/MS data and the large databases searched, the accuracy of peptide identification algorithms remains limited. To improve the accuracy of identification we applied a machine-learning approach using a hidden Markov model (HMM) to capture the complex and often subtle links between a peptide sequence and its MS/MS spectrum.
Model: Our model, HMM_Score, represents ion types as HMM states and calculates the maximum joint probability for a peptide/spectrum pair using emission probabilities from three factors: the amino acids adjacent to each fragmentation site, the mass dependence of ion types and the intensity dependence of ion types.
The identification of proteins by tandem mass spectrometry relies on knowledge of the products produced by collision-induced dissociation of peptide ions. Most previous work has focused on fragmentation statistics for ion trap systems. We analyzed fragmentation in MALDI TOF/TOF mass spectrometry, collecting statistics using a curated set of 2459 MS/MS spectra and applying bootstrap resampling to assess confidence intervals.
View Article and Find Full Text PDFMatern Child Health J
November 2006
Objectives: The study was undertaken to identify the extent of missed opportunities of clients for selected reproductive and child health services, including the reasons and factors contributing to missed opportunities, in NGO clinics in Bangladesh.
Methods: A cross-sectional survey of 6 NGO clinics from 6 randomly-selected municipality areas was conducted for one month during July-August 2000. In total, 1,478 clients were consecutively selected both from fixed and satellite clinics.
J Health Popul Nutr
December 2005
Depot-holders are women from the community who promote good health practice and use of clinics. They keep a stock of contraceptives and oral rehydration salts to supply other women and are paid some incentives. In 2003, the NGO Service Delivery Program (NSDP) introduced depot-holders in three types of urban area in Bangladesh as a pilot.
View Article and Find Full Text PDFHealth Care Manag (Frederick)
February 2005
A client-retained Family Health Card (FHC) was distributed to all households of 2 districts of Bangladesh under the Health and Population Sector Programme. This study assessed the extent and factors relating to retention of FHC, its perceived usefulness, and the extent of use. Quantitative and qualitative data were collected from randomly selected subdistricts.
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