Corynebacterium spp.: relationship of pathogenic properties and antimicrobial resistance.

Corynebacterium spp. are part of the human microbiome, but can cause the development of inflammatory diseases of various localization. Purpose - to evaluate the relationship between pathogenic properties and resistance to antimicrobial drugs (AMD) of Corynebacterium spp.

Possibilities of transmission electron microscopy in assessing the quality of bacterial cells and microbial population.

Determination of changes in the morphostructure of microorganisms under the influence of antibiotics, disinfectants, biocins, physical and chemical agents is carried out using the method of visualization of ultrathin sections in a transmission electron microscope. The aim is to develop methodological techniques for express preparation and visualization of the ultrastructure of bacteria in a transmission electron microscope to detect structural damage to cells and assess the quality of the microbial population. A technique of accelerated sample preparation for visualization of the fine structure of bacteria and diagnosis of structural damage to cells in a transmission electron microscope has been developed.

Etiological significance of Corynebacterium spp. in the development of diseases of the respiratory tract.

Corynebacterium spp. It is associated with inflammatory diseases of the respiratory tract (tracheitis, pharyngitis, rhinosinusitis, bronchitis, pneumonia, etc.).

Peculiarities of the ultrastructure of mixed biofilms of the causing agent of diphtheria and conditionally pathogenic microorganisms isolated from the human respiratory tract.

Unlabelled: In the post-epidemic period, the circulation of the causative agent of diphtheria in the population is maintained due to the carrier of bacteria. Entering an organism with a high level of antitoxic immunity, the pathogen enters into intermicrobial interactions with representatives of the opportunistic microflora inhabiting the respiratory tract and forms a biofilm.

Materials And Methods: Modeling of the biofilm formation process was carried out using the strains C.

Corynebacterium spp. - problematic pathogens of the human respiratory tract (review of literature).

Corynebacterium spp. - representatives of the normal microflora of the human body, but their role in the development of diseases in both immunocompromised and immunocompetent patients is known. Corynebacterim spp.

Diphtheria: characteristics of the pathogen and laboratory diagnostics (lecture).

The problem of diphtheria infection remains relevant, since the circulation of toxigenic strains of Corynebacterium diphtheriae persists in the body of bacterial carriers, despite ongoing vaccination. The lecture presents modern ideas about the properties of the pathogen, its pathogenicity factors (toxin, pili, surface proteins (67-72P (or DIP0733), DIP1281, etc.) and their role in the pathogenesis of the disease.

[Cypopathic effect of diphtheria pathogen in the composition of biofilm.].

When the nasopharynx is colonized with toxigenic strains of the diphtheria pathogen, toxin is released, which contributes to the death of epithelial cells. But in bacterial carriers, the development of the clinical picture of the disease does not occur. This is due to the peculiarities of the state of their immune system, as well as the peculiarities of the production of diphtheria exotoxin by corynebacteria in the biofilm.

[Analysis of the frequency of allocation of Corynebacteria non-diphtheria from patients with inflammatory diseases of the respiratory tract.].

Corynebacteria non-diphtheria and, in particular, C. pseudodiphtheriticum species that are closely related to C. propinquum and C.

[Sensitivity and resistance to antimicrobial agents ESBL-producing and not producing ESBL strains of E. coli in patients with urinary tract infection.].

As a result of the conducted researches it is shown that 44.1% of urinary tract infections (UTIS) caused by E. coli are accounted for by producers of beta-lactamase of the extended spectrum of action (ESBL).

[The sensitivity to antibiotics of Corynebacterium non diphtheriae isolated in hospitals of Rostov-on-Don and the Rostovskaia oblast.].

The purpose of study is to analyze sensitivity of various species Corynebacterium non diphtheriaе to antibacterial pharmaceuticals. The strains of C. non diphtheriae are separated from patients with pathology of respiratory and urogenital tract and also from individuals subjected to preventive examination.

[The antibiotic sensitivity of Corynebacterium diphtheriaе gravis tox+ in composition of mixed biofilms.].

The purpose of study is to determine sensibility of strains of C. diphtheriae gravis tox+ in the composition of mixed biofilms to antibacterial medications. The object of study was strains of Corynebacterium diphtheriae gravis tox+ № 665, Corynebacterium pseudodiphtheriticum (poly-resistant), Corynebacterium pseudodiphtheriticum (antibiotic-resistant).

ANTIBIOTICS RESISTANCE OF CORYNEBACTERIUM NON DIPHTHERIAE STRAINS.

Aim: Study the frequency of occurrence of antibiotics resistant strains of various species of Corynebacterium non diptheriae.

Materials And Methods: C.pseudodiphtheriticum, C.

[Corynebacterium riegelii as an unusual species separated from female patient with tuberculosis.].

The article describes a clinical case of tuberculosis of of urinary tracts of a female patient with massive discharge of strain Corynebacterium riegelii from urogenital tract. The data of clinical laboratory examination of female patient are analyzed. The characteristics of isolated strain C.

[CORYNEBACTERIUM: FEATURES OF THE STRUCTURE OF THE BACTERIAL CELL].

In a review of the features of the bacterial cells are Corynebacterium structure: characterized by an upper layer, highly organized cell wall, cytoplasmic membrane, cytoplasm, nucleoid. Described in detail the structure of the upper layer containing pili (fimbriae), microcapsule surface proteins - PS-2, DIP1281, 67-72r protein (hemagglutinin), porins, sialidase (neuraminidase). These components are the ability to initiate a serial of Corynebacterium work with the host cell, followed by colonization.

[PATHOGENICITY FACTORS OF CORYNEBACTERIUM NON DIPHTHERIAE].

Pathogenicity factors of Corynebacterium non diphtheriae - pili, microcapsule, cell wall, pathogenicity enzymes, toxins, that determine the ability of microorganisms to consequentially interact with epithelium of entry gates of the organism, replicate in vivo, overcome cell and hu- moral mechanisms of protection, are examined in the review. Particular attention in the paper is given to species of non-diphtheria corynebacteria, that are pathogenic for human and able to produce toxins - Corynebacterium ulcerans and Corynebacterium pseudotuberculosis. Mechanisms of expression regulation of PLD-exotoxins, its interaction with immune system cells are described.

[THE COMPARATIVE ANALYSIS OF TECHNIQUES OF IDENTIFICATION OF CORYNEBACTERIUM NON DIPHTHERIAE].

The comparative analysis was carried out concerning effectiveness of three techniques of identification of Corynebacterium non diphtheriae: bacteriological, molecular genetic (sequenation on 16SpRNA) andmass-spectrometric (MALDI-ToFMS). The analysis covered 49 strains of Corynebacterium non diphtheriae (C.pseudodiphheriticum, C.

[The methods of identification of Corynebacterium non diphtheria].

The proper identification of Corynebacterium non diphtheriae is complicated by their wide species variety, variable biochemical activity, large spectrum of diseases at which they are isolated and also by their presence in normal human micro-flora. The bacteriological method is a traditional basis for identification of corynebacteria though long (7-14 days), produces ambiguous results at cultivation of lipophilic and biochemically variable species. For final identification of nondescript species of Corynebacterium non diphtheriae it is recommended to carry out molecular genetic study using golden standard - sequencing on 16S pRNA (DNA), genes rpoB and PLD.

[THE DIAGNOSTIC APPROACHES TO VERIFICATION OF STREPTOCOCCUS INFECTION IN PATIENTS WITH INFECTIOUS MONONUCLEOSIS].

The Rostovskii state medical university of Minzdrav of Russia, 344022 Rostov-on-Don, Russia The analysis is applied concerning significance of laboratory techniques of verification of streptococcus infection (bacteriological analysis, detection of anti-streptolysin O in pair serums) in 148 patients with infectious mononucleosis aged from 3 to 15 years. The content of anti-streptolysin O exceeded standard in 41 ± 4.8% of patients with concomitant in acute period and in 49.

[Features of biofilm morphology, formed by Corynebacterium diphtheriae gravis tox+ strains].

Aim: Study the structure of homogenous microbial communities of Corynebacterium diphtheriae gravis tox+ strains during formation of biofilms in vitro.

Materials And Methods: Object of study--typical and biofilm cultures of C. diphtheriae gravis tox+ museum and circulating strains.

[Effect of Corynebacterium non diphtheriae on functional activity and apoptosis of macrophages].

Aim: Determine the ability of Corynebacterium non diphtheriae to induce phagocytosis and apoptosis of macrophages and evaluate regulatory effect of nuetrophilokines (NPK) induced by Corynebacterium non diphtheriae on these processes.

Materials And Methods: The ability of Corynebacterium non diphtheriae, isolated from upper respiratory tract, skin and urogenital tract (UGT) were studied for the ability to induce phagocytosis and apoptosis of mice macrophages (MP; in vitro during staining by May-Grunwald with additional staining by Romanowsky-Giemsa) before and after the addition of NPK induced by Corynebacterium non diphtheriae.

Results: Phagocytic index (PI) was the same for all the Corynebacterium non diphtheriae species, phagocytic number (PN) and index of phagocytosis completion (IPC)--were minimal relative to corynebacteria isolated from UGT.

[Apoptogenic activity of microbes-associants during Epstein-Barr virus infection].

Aim: Study apoptogenic activity of-microbes-associants during Epstein-Barr virus infection (EBVI) on the model of mice peritoneal macrophages in vitro.

Materials And Methods: Evaluation of apoptosis induced by bacteria isolated from EBVI patients was carried out by characteristic morphological changes of macrophages in smears stained by May-Grunwald with additional staining by Romanowsky-Giemsa.

Results: All the EBVI microbes-associants were established to have apoptogenic activity, however, the highest pathogenic potential was noted in Streptococcus pyogenes.

[The comparative characteristic of immune status of males with bacterial inflammatory urogenital pathology of different etiology in the city of Orenburg].

The article discusses presence of typical characteristics of parameters of system immunity under gonococcal and nonspecific uretroprostatitis and diagnostic value of these indicators. The reliable differences of immunologic indicators in patients with gonorrhea are established as compared to patients with nonspecific bacterial uretroprostatitis. The study established in peripheral blood the reliable decrease of level of leukocytes, relative amount of monocytes, phagocyte index, functional reserve of leukocytes at the expense of spontaneous and stimulated NBT test, IgA, sIgA.

[Adhesion of corynebacterium diphtheriae: the role of surface structures and formation mechanism].

The paper is devoted to the study of surface structures including pili (fimbriae) 67-72p surface protein, DIP 1281 surface protein, lipoarabinomannan CdiLAM and their role in the adhesion and colonization of the mucous membrane of the throat by Corynebacterium diphtheriae. A description is offered for the main stages in the adhesion process of diphtheria causative agent and the ability of its adhesins to stimulate the effect of innate and acquired immunity factors. The paper stresses prospectiveness of the development of vaccines forming immunoprotection of the organism against adhesive activity of C.

[Mechanisms of formation of post-vaccinal immune response in children immunized with APDT and ADT-M preparations].

Aim: Study the mechanisms of formation of cell and humoral immunity against pertussis, diphtheria and tetanus in children immunized with immunobiological preparations (APDT vaccine and ADT anatoxin).

Materials And Methods: 30 practically healthy children (6 - 9 years of age) immunized with APDT and ADT-M preparations had TLR2, TLR4 expression determined in mononuclear cells (MNC). Vaccine preparations (APDT, ADT-M, AD-M, AT) and Corynebacterium diphtheriae gravis tox+, C.

[The ability of diphtheria causative agent to form biofilm].

The article deals with results of studying diphtheria causative agent capacities to form biofilm as one of mechanisms of persistence in human organism. The study object was strain of C.diphtheriae gravis tox+ obtained from nasopharynx of patient aged 19 in municipal hospital No 1 of town of Gukovo of Rostov oblast in 2011.