Characterisation of the Self-Sufficient Cytochrome P450 CYP116B234 From Rhodococcus globerulus and Its Suggested Native Role in 2-Hydroxyphenylacetic Acid Metabolism.

Cytochromes P450 (P450s) are exceptional biocatalysts that enable the selective oxidation of unactivated C-H bonds using molecular oxygen. Typically, auxiliary redox partner proteins deliver electrons from NAD(P)H to the P450, enabling oxygen activation. However, associating native redox partners with P450s can be challenging, particularly when they are genomically separated.

Deciphering the seasonal dynamics of microplastic morphotypes and associated co-contaminants along the northwest coast of India.

In the present study, the northwest coast of India, bordering the Arabian Sea, was selected to evaluate the microplastic (MP) abundance. This is the first study to emphasize the effects of different seasons on MP distribution. The collected MPs were dried, segregated, and evaluated based on their morphotype, size, color, and polymer type.

Improved production of alkaline and solvent-stable proteases from a halotolerant Exiguobacterium isolate through heterologous expression.

Halophiles are excellent sources of detergent proteases that are attributed to stability in alkaline pH, salts, surfactants, and hydrophobic solvents. The lower enzymatic yields and tedious downstream processes necessitate the search for newer halophilic sources. We have previously reported a halotolerant Exiguobacterium sp.

Screening of Multitarget-Directed Natural Compounds as Drug Candidates for Alzheimer's Disease Using Techniques: Their Extraction and Validation.

Alzheimer's disease (AD) is a neurodegenerative disorder that impairs neurocognitive function. Acetylcholinesterase (AChE) and β-site APP cleaving enzyme 1 (BACE1) are the two main proteins implicated in AD. Indeed, the major available commercial drugs (donepezil, rivastigmine, and galantamine) against Alzheimer's are AChE inhibitors.

Pan-genomic comparison of a potential solvent-tolerant alkaline protease-producing sp. TBG-PICH-001 isolated from a marine habitat.

Unlabelled: The identification and applicability of bacteria are inconclusive until comprehended with genomic repositories. Our isolate, sp. TBG-PICH-001 exhibited excellent halo- and organic solvent tolerance with simultaneous production of alkaline protease/s (0.

Biodegradation of terephthalic acid using Rhodococcus erythropolis MTCC 3951: Insights into the degradation process, applications in wastewater treatment and polyhydroxyalkanoate production.

Terephthalic acid (TPA) is an endocrine disruptor widely used as a plasticizer and as a monomer in the manufacturing of PET bottles. However, because of various harmful effects on humans and the environment, it is now recognized as a priority pollutant whose environmental level needs to be controlled. In the present work, the TPA biodegradation efficacy of the bacterium Rhodococcus erythropolis (MTCC 3951) was studied in mineral salt media with TPA as the sole carbon and energy source.

Ensemble Wavelet Decomposition-Based Detection of Mental States Using Electroencephalography Signals.

Technological advancements in healthcare, production, automobile, and aviation industries have shifted working styles from manual to automatic. This automation requires smart, intellectual, and safe machinery to develop an accurate and efficient brain-computer interface (BCI) system. However, developing such BCI systems requires effective processing and analysis of human physiology.

Whole genome sequencing and functional analysis of a novel biofilm-eradicating strain Nocardiopsis lucentensis EMB25.

The process of biofilm formation is intricate and multifaceted, requiring the individual cells to secrete extracellular polymeric substances (EPS) that subsequently aggregate and adhere to various surfaces. The issue of biofilms is a significant concern for public health due to the increased resistance of microorganisms associated with biofilms to antimicrobial agents. The current study describes the whole genome and corresponding functions of a biofilm inhibiting and eradicating actinobacteria isolate identified as Nocardiopsis lucentensis EMB25.

Monitoring the lipid oxidation and fatty acid profile of oil using algorithm-assisted surface-enhanced Raman spectroscopy.

Deep-fat frying of food develops lipid oxidation products that deteriorate oil and pose a health risk. This necessitates the development of a rapid and accurate oil quality and safety detection technique. Herein, surface-enhanced Raman spectroscopy (SERS) and sophisticated chemometric techniques were used for rapid and label-free determination of peroxide value (PV) and fatty acid composition of oil in-situ.

Inhibition and eradication of biofilms by secondary metabolites of EMB25.

Millions of people worldwide have been impacted by biofilm-associated disorders, which are impregnable owing to frequent changes in surface antigens and gene expression. Globally, about 11% of nosocomial infections, including cystic fibrosis, chronic wound infections, and post-surgical infections, are caused by , the most prevalent Gram-negative bacterial species. Moreover, biofilms are highly resistant to the host's immune system, and exhibit increased tolerance to stress factors such as starvation, dehydration, and antimicrobials.

Transglutaminase-Polyethyleneimine Nanoflowers Mediated Cellular Delivery of Anti-miR-210 for Effective Glioblastoma Therapy.

Glioblastoma (GBM) is a deadly tumor of the central nervous system (CNS) having a dismal prognosis. miRNA-based therapeutics hold immense potential for GBM therapy; however, its delivery remains a daunting challenge. MicroRNA-210 has been established as a critical oncomiR in GBM.

Computational Exploration of Bio-Degradation Patterns of Various Plastic Types.

Plastic materials are recalcitrant in the open environment, surviving for longer without complete remediation. The current disposal methods of used plastic material are inefficient; consequently, plastic wastes are infiltrating the natural resources of the biosphere. The mixed composition of urban domestic waste with different plastic types makes them unfavorable for recycling; however, natural assimilation in situ is still an option to explore.

An explainable and interpretable model for attention deficit hyperactivity disorder in children using EEG signals.

Background: Attention deficit hyperactivity disorder (ADHD) is a neurodevelopmental disorder that affects a person's sleep, mood, anxiety, and learning. Early diagnosis and timely medication can help individuals with ADHD perform daily tasks without difficulty. Electroencephalogram (EEG) signals can help neurologists to detect ADHD by examining the changes occurring in it.

SchizoNET: a robust and accurate Margenau-Hill time-frequency distribution based deep neural network model for schizophrenia detection using EEG signals.

Schizophrenia (SZ) is a severe chronic illness characterized by delusions, cognitive dysfunctions, and hallucinations that impact feelings, behaviour, and thinking. Timely detection and treatment of SZ are necessary to avoid long-term consequences. Electroencephalogram (EEG) signals are one form of a biomarker that can reveal hidden changes in the brain during SZ.

Efficient remediation of meropenem using Bacillus tropicus EMB20 β-lactamase immobilized on magnetic nanoparticles.

Reducing antibiotic pollution in the environment in essential to preserve the effectiveness of the available antibiotics. In the present study, β-lactamase from Bacillus tropicus EMB20 was immobilized onto magnetic nanoparticles (FeO) through covalent coupling method. The nanoconjugate was structurally characterized using SEM, FTIR, UV-spectrometry, and XRD diffraction analyses.

An Intelligent Motor Imagery Detection System Using Electroencephalography with Adaptive Wavelets.

Classification of motor imagery (MI) tasks provides a robust solution for specially-abled people to connect with the milieu for brain-computer interface. Precise selection of uniform tuning parameters of tunable Q wavelet transform (TQWT) for electroencephalography (EEG) signals is arduous. Therefore, this paper proposes robust TQWT for automatically selecting optimum tuning parameters to decompose non-stationary EEG signals accurately.

Insights from the genome sequence of EMB20, an efficient β-lactamase-producing bacterium.

Unlabelled: We report here the whole-genome sequence of β-lactamase-producing bacteria EMB20. The genome sequence of EMB20 has a size of 5.8 Mb (G + C content of 35.

Investigation of Streptomyces sp. Strain EMB24 Secondary Metabolite Profile Has Unraveled Its Extraordinary Antibacterial Potency Against Drug-Resistant Bacteria.

With the overuse and misuse of antibiotics amid COVID-19 pandemic, the antimicrobial resistance, which is already a global challenge, has accelerated its pace significantly. Finding novel and potential antibiotics seems one of the probable solutions. In this work, a novel Streptomyces sp.

Production of lactones for flavoring and pharmacological purposes from unsaturated lipids: an industrial perspective.

The enantiomeric pure and natural (+)-Lactones (C ≤ 14) with aromas obtained from fruits and milk are considered flavoring compounds. The flavoring value is related to the lactones' ring size and chain length, which blend in varying concentrations to produce different stone-fruit flavors. The nature-identical and enantiomeric pure (+)-lactones are only produced through whole-cell biotransformation of yeast.

Microbial transglutaminase nanoflowers as an alternative nanomedicine for breast cancer theranostics.

Breast cancer is the most common malignancy among women. With the aim of decreasing the toxicity of conventional breast cancer treatments, an alternative that could provide appropriate and effective drug utilization was envisioned. Thus, we contemplated and compared the effects of microbial transglutaminase nanoflowers (MTGase NFs) on breast cancer cells (MCF-7).

Genome Sequence Analysis of sp. Strain TBG-PICH-001, Isolated from Pichavaram Mangrove Forest in South India.

The draft genome sequence of sp. strain TBG-PICH-001, isolated from Pichavaram Mangrove Forest (Tamil Nadu, India), is reported here. Paired-end sequencing technology was used to sequence the genome on the Illumina HiSeq X Ten platform.

Molecular and structural insights of β-boswellic acid and glycyrrhizic acid as potent SARS-CoV-2 Envelope protein inhibitors.

Background: Over million people have been infected with SARS-CoV-2 virus worldwide, with around 3% reported deaths till date. A few conventional antiviral treatments have been tried to mitigate the coronavirus. However, many alternative therapeutics are being evaluated worldwide.

Effect of leaves extracts on thermo-lipid oxidation and Maillard pathway born food toxicant acrylamide in Indian staple food.

Unlabelled: Deep frying of food is a common practice that leads to the formation of lipid oxidation products. These lipid oxidation products have a role in the Maillard reaction, which ultimately leads to the formation of cancer-causing and neurotoxic substance acrylamide. In this regard, the leaves extract-treated sunflower oil on oxidative stability and acrylamide content in  a popular deep-fried staple food in India were studied and compared with synthetic antioxidant butylated hydroxytoluene (BHT) till four frying cycles.