mRNA encoding Sec61β, a tail-anchored protein, is localized on the endoplasmic reticulum.

Although one pathway for the post-translational targeting of tail-anchored proteins to the endoplasmic reticulum (ER) has been well defined, it is unclear whether additional pathways exist. Here, we provide evidence that a subset of mRNAs encoding tail-anchored proteins, including Sec61β and nesprin-2, is partially localized to the surface of the ER in mammalian cells. In particular, Sec61b mRNA can be targeted to, and later maintained on, the ER using both translation-dependent and -independent mechanisms.

[State of the liver antioxidant system and content of matrix metalloproteinase-2 of the large intestine under the effect of maleimide derivative in experimental colorectal carcinogenesis in rats].

The maleimide derivative--1-(4-Cl-benzyl)-3-Cl-4-(CF3-phenylamino)-1H-pyrrol-2.5-dione (MI-1) with cytostatic activity did not cause substantial changes of liver antioxidant system and level of matrix metalloproteinase-2 in intestinal mucosa after chronic treatment (for 20 weeks). MI-1 did not cause significant changes in the content of thiobarbituric-active products and plasma membrane protein carbonyl groups in the rat liver.

[Effect of maleimide derivative on the morpho-functional state of the kidney in experimental colon carcinogenesis in rats].

The effect of long-term administration of the novel maleimide derivative with antiproliferative activity on kidney morpho-functional state in experimental (1,2-dimethylhydrazine-induced) colon carcinogenesis has been investigated on 60 male rats. 1,2-Dimethylhydrazine was injected subcutaneously in dose 20 mg/kg one time per week during 20 weeks. Maleimide derivative in dose of 0,027 and 2,7 mg/kg was given per os daily during the same time.