Nanoliter droplet vitrification for oocyte cryopreservation.

Aim: Oocyte cryopreservation remains largely experimental, with live birth rates of only 2-4% per thawed oocyte. In this study, we present a nanoliter droplet technology for oocyte vitrification.

Materials & Methods: An ejector-based droplet vitrification system was designed to continuously cryopreserve oocytes in nanoliter droplets.