Corosolic acid and its derivatives targeting MCCC1 against insulin resistance and their hypoglycemic effect on type 2 diabetic mice.

Corosolic acid (CA), a natural triterpenoid, exhibits various biological activities and is often called as plant-derived insulin due to its significant hypoglycemic effects, making it especially beneficial for individuals with diabetes or high blood glucose levels. However, CA has notable in vitro toxicity, low water solubility, and poor pharmacokinetic properties. To address these limitations, a series of CA derivatives were synthesized, resulting in the identification of derivative H26, which demonstrates a significantly enhanced hypoglycemic effect, reduced toxicity, and improved pharmacokinetic characteristics compared to CA.

A Cross-Sectional Serological Study to Assess the Prevalence and Risk Factors of Anaplasmosis in Dromedary Camels in Punjab, Pakistan.

Anaplasmosis is an infectious disease transmitted by ticks and caused by obligate intracellular pathogen of belonging to genus Infections of one-humped camels () and llamas () have been reported previously. The aim of this study was to investigate the seroprevalence and risk factors of anti- spp. antibodies in of the Punjab, Pakistan.

Therapeutic Utility of Mulligan Traction Straight Leg Raise Stretch and Proprioceptive Exercises in Osteoarthritis Treatment.

Background Given the increasing incidence and severity of knee osteoarthritis (OA), it is crucial to investigate and refine therapeutic approaches. Aim The objective of this study is to evaluate the effectiveness and potential synergistic effects of proprioceptive exercises combined with Mulligan traction straight leg raise (MT-SLR) in treating OA. This includes improving symptoms such as functional mobility, pain reduction, and relevant serological markers, highlighting the potential of this approach to enhance overall patient outcomes.

Determinants of Physical Activity Adoption and Maintenance in Older Adults: A Dual Process Approach.

Background: Dual process models represent a useful framework in explaining PA in that behavior is explained by reflective (i.e., conscious, effortful) and automatic (i.

Quantitative LC-QToF-MS Analysis of Mycochemicals in Amanita muscaria, Psilocybe spp. (Agaricomycetes), and Consumer Products.

The psychedelic mushroom market has expanded rapidly due to changing regulations and increasing consumer demand. Product diversity now extends beyond traditional capsules and tablets to include gummies, powders, and confectionery items, complicating quality control efforts. To assess the quality and potential adulteration of Amanita musca-ria and Psilocybe cubensis-based products, a validated LC-QToF-MS method was developed.

Impact of the CAPTURE COPD Screening Tool in US Primary Care: A Cluster Randomized Trial.

The impact of COPD screening on US primary care clinician behavior and patient outcomes is unclear. Assess the impact of receiving CAPTURE (COPD Assessment in Primary Care to Identify Undiagnosed Respiratory Disease and Exacerbation Risk) screening scores on clinical and patient outcomes. Cluster randomized trial included 49 usual care (COPD education only) and 51 intervention (COPD and CAPTURE education plus screening scores) primary care practices.

Optimizing broiler performance, carcass traits, and health: evaluating thyme and/or garlic powders as natural growth promoters in antibiotic-free diets.

The purpose of this study was to evaluate the effects of thyme powder (TP), garlic powder (GP), and their combination as natural alternatives to antibiotics on broiler growth performance, blood indices, and carcass characteristics. A completely randomized design was used, with four experimental groups consisting of 264 one-day-old Ross 308 broiler chicks (45.27±0.

Optimization of Binding Buffer Composition (Polyethylene Glycol, Sodium Chloride and pH) for Extraction of DNA from Biological Fluids Using Polyethyleneimine Functionalized Iron Oxide Nanoparticle-Based Method.

Introduction: Efficient extraction of DNA from biological fluids is crucial for applications in molecular biology, forensic science, and clinical diagnostics. However, traditional DNA extraction methods often require costly reagents and lengthy procedures. This study aims to optimize the binding buffer composition for DNA extraction using polyethyleneimine-coated iron oxide nanoparticles (PEI-IONPs), which offer the dual benefits of magnetic separation and high DNA-binding efficiency.

Frequency of Acute Kidney Injury in Patients Admitted With Acute Stroke at Hayatabad Medical Complex, Peshawar.

Background Patients hospitalized with acute stroke are at risk of developing acute kidney injury (AKI), and when both conditions occur together, patient outcomes are often worse. The relationship between stroke type, patient characteristics, and the development of AKI is not fully understood, particularly in tertiary care settings in Pakistan, where healthcare resources and patient characteristics may differ from Western populations. Objective To determine the frequency of AKI and identify associated risk factors, including stroke type, severity, and comorbidities, in patients presenting with acute stroke at a tertiary care center in Pakistan, the Hayatabad Medical Complex, Peshawar.

Silver nanoparticle toxicity in rainbow trout: insights into physiological and molecular responses.

Metallic nanoparticles, with their large surface area to volume ratio, are increasingly important in various life fields, but they can cause varying toxic effects on fish. This study investigates the toxicological effects of silver nanoparticles (AgNPs) on rainbow trout (), focusing on hematological, biochemical, antioxidant, and histopathological changes. Fish were exposed to varying concentrations of AgNPs (0.

Burden of neurodevelopmental disorder in Lakki Marwat population of Khyber Pakhtunkhwa, Pakistan.

Background: Pakistan is a multi-cultural country with different ethnic groups living in both rural and urban communities. There is a significant occurrence of congenital and hereditary neurodevelopmental disorder (NDD) contributing to mortality and morbidity rates.

Objectives: This study aims to explore the prevalence, patterns, and phenotypic spectrum of NDD in district Lakki Marwat population of Khyber Pakhtunkhwa, Pakistan.

Rectosigmoid Junction Cancer; The Role of Preoperative and Postoperative Radiation With Novel Nomogram in Predicting Survival in the United States.

Background: There is controversy and limited data the management of rectosigmoid junction cancer (RSJC), especially the role of radiation. We aim to investigate the role of preoperative and postoperative radiation in RSJC and whether this cancer should be treated as a colon cancer or as a rectal cancer.

Methods: The data were extracted from the Surveillance, Epidemiology, and End Results (SEER) database and identified from 2000 to 2018.

Novel homozygous SPAG17 variants cause human male infertility through multiple morphological abnormalities of spermatozoal flagella related to axonemal microtubule doublets.

Male infertility can result from impaired sperm motility caused by multiple morphological abnormalities of the flagella (MMAF). Distinct projections encircling the central microtubules of the spermatozoal axoneme play pivotal roles in flagellar bending and spermatozoal movement. Mammalian sperm-associated antigen 17 (SPAG17) encodes a conserved axonemal protein of cilia and flagella, forming part of the C1a projection of the central apparatus, with functions related to ciliary/flagellar motility, skeletal growth, and male fertility.

Molecular Functional and Transcriptome Analysis of Overexpression from Zicaitai ( var. ).

B-box transcription factors (TFs) in plants are essential for circadian rhythm regulation, abiotic stress responses, hormonal signaling pathways, secondary metabolism, photomorphogenesis, and anthocyanin formation. Here, by blasting the gene sequence, we identified a total of 18 genes from five distinct species (, , , , and ). The gene is most closely linked to the gene based on phylogeny and protein sequence similarities.

Cooled Multifunctional Platforms to Alleviate Heat Stress in Broiler Chickens: Effects on Performance, Carcass and Meat Quality Traits.

Platforms have been shown to be a suitable environmental enrichment for broiler chickens, accommodating their motivation to roost and rest at an elevated position. In order to increase the animal welfare benefits, we designed prototype elevated platforms with additional functionalities: a local cooling system, a sheltered area underneath the platform and collection trays underneath the platform that prevent manure from falling on the litter. This study assessed the effects of these multifunctional platforms during thermoneutral and heat stress conditions on two key determinants of their commercial uptake potential, namely production performance, carcass and meat quality.

Ameliorated delivery of amphotericin B to macrophages using chondroitin sulfate surface-modified liposome nanoparticles.

Background: The neglected tropical disease leishmaniasis has significant adverse effects from current treatments and limited therapeutic options are currently available.

Objective: The aim of this study was to develop a surface-modified nano-liposomal drug delivery system, anchored with chondroitin sulfate (CS), to effectively transport Amphotericin B (AmB) to macrophages.

Methods: Conventional liposome formulations (CL-F) and CS-coated surface-modified liposome formulations (CS-SML-F) were formulated by the thin film hydration method and characterized for particle size, polydispersity index (PDI), zeta potential, and entrapment efficiency with long-term stability.

Assessing the Efficacy of Modified CT Severity Index Versus Conventional CT Severity Index in Determining Severity and Clinical Outcomes of Chronic Obstructive Pulmonary Disease (COPD).

Aims The aim of this study was to evaluate the severity of chronic obstructive pulmonary disease (COPD) using the computed tomography severity index (CTSI) and the modified CTSI (MCTSI) and to assess their correlation with clinical outcome measures. Additionally, the study aimed to compare the diagnostic performance of these indices in predicting moderate to severe COPD, based on patient outcomes. Materials and methods In this prospective study, conducted between November 2023 and March 2024, two radiologists, blinded to clinical outcomes, independently assessed CTSI and MCTSI.

Demographics, Prognostic Factors, and Survival Outcomes in Hepatic Angiosarcoma: A Retrospective Analysis.

Background: Hepatic angiosarcoma (HA) is a rare malignant vascular neoplasm. Currently, there are no standardized protocols for treating HA. This study aims to understand clinicopathologic analysis, prognostic factors, and treatment outcomes comprehensively.

Genipin-enriched chitosan-Zein nanoparticles for improved curcumin encapsulation.

Polysaccharide-protein nanocomplexes are considered one of the desired systems to encapsulate bioactive compounds. The study prepared chitosan (CS)/genipin (GP)/zein nanoparticles with chemical cross-linkage for encapsulating and releasing curcumin. Fourier transform infrared spectra demonstrated that cross-linkage between molecules was attributed to electrostatic interactions and the formation of amido bonds and hydrogen bonding.

Targeting human plasma cells using small molecule regulated BCMA CAR T cells eliminates circulating antibodies in humanized mice.

Pathogenic long-lived plasma cells (LLPCs) secrete autoreactive antibodies, exacerbating autoimmune diseases and complicating solid organ transplantation. Targeted elimination of the autoreactive B-cell pool represents a promising therapeutic strategy, yet current treatment modalities fall short in depleting mature plasma cells. Here, we demonstrate that chimeric antigen receptor (CAR) T cells, targeting BCMA utilizing a split-receptor design, offer a controlled and effective therapeutic strategy against LLPCs.

Impact of activation energy on carreau nanofluid flow over non-linear stretching surface.

- The main goal of this article is to outline the key elements of activation energy that govern the slip flow of Carreau fluid over a non-linear stretched surface with heat transfer. By using the Arrhenius relation, activation energy aspects are included. Brownian diffusion and thermophoretic effects are shown through the study of nanoparticle dispersion using the Buongiorno model.

Impaired myofibroblast proliferation is a central feature of pathologic post-natal alveolar simplification.

Premature infants with bronchopulmonary dysplasia (BPD) have impaired alveolar gas exchange due to alveolar simplification and dysmorphic pulmonary vasculature. Advances in clinical care have improved survival for infants with BPD, but the overall incidence of BPD remains unchanged because we lack specific therapies to prevent this disease. Recent work has suggested a role for increased transforming growth factor-beta (TGFβ) signaling and myofibroblast populations in BPD pathogenesis, but the functional significance of each remains unclear.

Cesarean Section Trends and Associated Factors at a Tertiary Care Center in India: A Retrospective Study.

Background Cesarean section (CS) is one of the most common surgical procedures performed on women globally, and its prevalence has been rising significantly over the past few decades. CS rates have been increasing globally, raising public health concerns due to the associated financial burden and increased health risks compared to vaginal delivery. Methodology This study involves a retrospective analysis of delivery records from a tertiary care hospital in Uttar Pradesh, India, over 10 years, from January 2011 to December 2021.