Identification and computational analysis of conserved YXXΦ motifs in the pan-serotypes of dengue virus NS5 protein and their implications in host-virus interactions.

Dengue is a major global health challenge, caused by the dengue virus (DENV) and transmitted through the mosquito. The four DENV serotypes (DENV1-4) infect about 400 million people annually. The non-structural protein 5 (NS5) is the most conserved DENV protein, crucial for viral replication.

Potential of transcranial ultrasound- and near-infrared spectroscopy-based acute stroke imaging for decision-making on intravenous thrombolysis treatment.

Background: Mobile Stroke Units (MSU) shorten time to intravenous thrombolysis (IVT) and improve functional outcome, but they rely on computed tomography (CT) making them highly specialized and costly. Alternative technologies can potentially identify imaging-based IVT contraindications like intracranial hemorrhage (ICH) or malignancies (IM), e.g.

Synthesis, Structural Characterization, and Magnetic Properties of Two New Fe(III)Mn(III) 1D Bimetallic Compounds.

Two Fe(III)Mn(III) bimetallic compounds, [Tp*Fe (CN)][(Mnsalen)]·MeOH·MeCN () and [Tp*Fe (CN)][Mnsalphen]·MeOH·HO (), were prepared by assembling the tricyanometalate building block (TEA)N [Tp*Fe(CN)] [Tp* = hydrotris(3,5-dimethylpyrazol-1-yl)borate] and the respective Mn Schiff base precursors [salen = ,'-ethylenebis(salicylideneiminate), salphen = ,-bis(salicylidene)-1,2-phenylenediamine]. Both compounds exhibit a one-dimensional (1D) zigzag chain structure linked by cyanide bridges, forming a (-Fe-C≡N-Mn-N≡C-) motif. Magnetic studies show a gradual increase of χ values in both complexes upon lowering temperature, indicating ferromagnetic coupling between the Fe and Mn metal centers with Curie-Weiss constants of +1.

Profiling genetic mutations in the DNA damage repair genes of oral squamous cell carcinoma patients from Pakistan.

Herein, we reported mutations in five DNA Damage Repair (DDR) i.e., TP53, ATR, ATM, CHEK1 and CHEK2 involved in OSCC using NG-WES and their analysis using bioinformatics tools.

The effect of different parenting styles on the child behavior during the dental visit: observational longitudinal study.

Background: It is widely recognized that parental influence significantly shapes children's emotional health and behavioral patterns. The current study aims to evaluate the effect of different parenting styles on the child's behavior during the dental visit.

Methods: Sixty healthy children age ranging from 4 to 6 years old who needed class I restoration in one of their primary molars were selected from the outpatient clinic of the Pediatric Dentistry and Dental Public Health Department, Faculty of Dentistry, Alexandria University, Egypt.

Genome duplication in a long-term multicellularity evolution experiment.

Whole-genome duplication (WGD) is widespread across eukaryotes and can promote adaptive evolution. However, given the instability of newly formed polyploid genomes, understanding how WGDs arise in a population, persist, and underpin adaptations remains a challenge. Here, using our ongoing Multicellularity Long Term Evolution Experiment (MuLTEE), we show that diploid snowflake yeast (Saccharomyces cerevisiae) under selection for larger multicellular size rapidly evolve to be tetraploid.

Larger Zona Orbicularis Size on Magnetic Resonance Imaging Is Not Associated With Increased Resistance to Axial Distraction of the Hip Joint.

Purpose: To evaluate the relation between zona orbicularis (ZO) thickness on magnetic resonance imaging and distractibility of the hip in patients undergoing hip arthroscopy.

Methods: We conducted a retrospective review of primary hip arthroscopy patients treated for femoroacetabular impingement syndrome from December 2021 to September 2022. Prior to arthroscopy, hips were subjected to 100 lb of force (lbf) with fluoroscopic images taken to calculate joint space and overall distraction distance.

Enhanced Binary Classification of Gait Disorders Using a Machine Learning Majority Voting Approach.

This study introduces a machine learning-based methodology for classifying healthy individuals and those with gait disorders, employing a merged data set from 'GaitRec' and 'Gutenberg.' Key gait features were extracted from the normalized ground reaction force data that had 2435 subjects, including maximum peaks, load rate, lowest troughs, and area under the curve. Numerous machine learning models, such as Support Vector Machine, Logistic Regression, Random Forest, Gradient Boosting, K-Nearest Neighbor, Bagging, Adaboost, and Neural Network, were developed, and their hyperparameters were optimized using a grid search approach.

Predictive factors for insertion torque values in transalveolar sinus lift procedures.

Objectives: The objective of this retrospective study was to investigate the impact of bone-related factors such as initial bone height (IBH), imaginary pixel unit (IPU), bone diameter (BD), crestal cortical thickness (CCT), and sinus floor thickness (SFT) in transalveolar sinus lift procedures using threaded bone expanders without bone graft materials.

Materials And Methods: This retrospective cohort study was carried out on patients who had reported to the department of oral and maxillofacial surgery at Tishreen University between January 2018 and June 2024. The sample consisted of patients who had transalveolar sinus lift using motorized threaded bone expanders with simultaneous hybrid dental implants placement without bone graft materials.

A GPR-based framework for assessing corrosivity of concrete structures using frequency domain approach.

Ground-penetrating radar (GPR) is a prominent non-destructive testing (NDT) method for corrosivity evaluation in concrete structures. Most GPR interpretation methods rely solely on the absolute values of rebar reflection intensity, making them vulnerable to misinterpretation of the effects of complex factors. This study introduces a more comprehensive GPR data interpretation method, encompassing analysis in time and time-frequency domains.

Prevalence and risk factors of curable sexually transmitted and reproductive tract infections and malaria co-infection among pregnant women at antenatal care booking in Kenya, Malawi and Tanzania: a cross-sectional study of randomised controlled trial data.

Objectives: Malaria and curable sexually transmitted and reproductive tract infections (STIs/RTIs) are associated with adverse pregnancy outcomes. This study reports the prevalence and risk factors of curable STIs/RTIs, STI/RTI co-infection and STI/RTI and malaria co-infection among HIV-negative pregnant women at their first antenatal care visit in Kenya, Malawi and Tanzania.

Methods: HIV-negative pregnant women of all gravidae (n=4680) were screened for syphilis with point-of-care tests and treated if positive.

Pathogenicity of Highly Pathogenic Avian Influenza A/H5Nx Viruses in Avian and Murine Models.

The evolution and adaptation of highly pathogenic avian influenza (HPAI) viruses pose ongoing challenges for animal and public health. We investigated the pathogenic characteristics of the newly emerged H5N1/2022 and H5N8/2022 of clade 2.3.

Effect of Plyometric Exercises of Lower Limb on Strength, Postural Control, and Risk of Falling in Stroke Patients.

Stroke, a major contributor to long-term disability worldwide, often results in significant impairments in motor function. These impairments can include weakness, impaired balance, and decreased coordination, which can have a significant influence on one's quality of life and independence. Finding an effective protocol for rehabilitation to improve these points will decrease the impact of stroke and its coast of rehabilitation.

Does supplementation with pine bark extract improve cardiometabolic risk factors? A systematic review and meta-analysis.

Background: Supplementation with pine bark extract (PBE) may improve risk factors associated with cardiometabolic syndrome (CMS). The effects of PBE supplementation on cardiometabolic risk factors were evaluated in this systematic review and meta-analysis of randomized controlled trials (RCTs).

Methods: A comprehensive search of various databases was performed to identify relevant RCTs published up to September 2024.

Climate change and its environmental and health effects from 2015 to 2022: A scoping review.

Background: The rise in environmental pollutants has become a pressing global concern of international magnitude. Substantial evidence now demonstrates that escalating global temperatures and rising sea levels might exacerbate release of chemical pollutants into the environment which amplifies their toxicity. Existing research underscores the linkage between climate change and air pollution as driving forces, with increased mortality and morbidity.

Arterial stiffness in gestational diabetes: Latest insights.

Increased arterial stiffness is a known cardiovascular risk factor, associated with hypertension and acute coronary events. Gestational diabetes (GDM) is associated with the development of placental-mediated disorders and future cardiovascular morbidity, raising the possibility of an association with increased arterial stiffness (AS). Several studies have now investigated this association through the assessment of pulse wave velocity and augmentation index.

Cardiac function and neurological development in complicated monochorionic pregnancies: Current evidence and clinical implications.

Monochorionic twin pregnancies, characterized by a shared placenta and unique vascular architecture, face significantly elevated risks of perinatal complications compared to dichorionic and singleton pregnancies. This review examines the pathophysiology, cardiovascular, and neurological adaptations in three primary complications of monochorionic pregnancies: Twin-to-Twin Transfusion Syndrome (TTTS), Selective Fetal Growth Restriction (sFGR), and Twin Anemia Polycythemia Sequence (TAPS). TTTS disrupts hemodynamic balance, leading to distinct cardiac dysfunctions and increased neurodevelopmental injury (NDI).

Phytochemical-Mediated Green Synthesis of Silver, Copper, and Ag-Cu Bimetallic Nanoparticles Using Peganum harmala Demonstrating Advanced Catalytic, Antioxidant, and Biomedical Applications.

This study explores the green synthesis of silver (Ag-NPs), copper (Cu-NPs), and silver-copper bimetallic nanoparticles (Ag-Cu Bimetallic NPs) using the leaf extract of Peganum harmala, a sustainable and renewable plant source. This eco-friendly synthesis method avoids hazardous chemicals and minimizes waste, advancing the principles of green nanotechnology. Characterization demonstrated distinct properties; Ag-NPs, Cu-NPs, and Ag-Cu bimetallic NPs exhibited surface plasmon resonance peaks at 425 nm, 555 nm, and 525 nm, respectively, and crystallite sizes of 21.

Molecular biology of the novel anticancer medications: a focus on kinases inhibitors, biologics and CAR T-cell therapy.

Introduction: Cancer treatment underwent significant changes in the last few years with the introduction of novel treatments targeting the immune system.

Objectives: The objective of this review is to discuss novel anticancer drugs including kinase inhibitors, biologics and cellular therapy with CAR-T cells.

Methods: Most recent research articles were extracted from PubMed using keywords such as "kinases inhibitors", "CAR-T cell therapy".

Effect of printing orientation and resin thickness on flexural strength of direct 3D-printed aligners.

Background: Direct 3D-printed aligners served as a breakthrough era in clear aligner fabrication. Yet, there is a scarcity of studies evaluating their mechanical properties. The aim of this study was to compare direct 3D-printed aligners derived from different printing orientations (vertically, horizontally, 30, and 45 degrees) and thickness (0.

Antibody in Breastmilk Following Pertussis Vaccination in Three-time Windows in Pregnancy.

Background: Pertussis-containing vaccines are routinely offered in the UK at 16-32 weeks of gestation and have been shown to be safe and effective, but there remains debate about the best timing for vaccination. Most research into this has focused on serologic immunity, but breastmilk is also important in infant immunity, and the amount of IgA in breastmilk may impact mucosal immunity. It is important to understand if the timing of vaccination in pregnancy affects the concentration of IgA in breastmilk.

Maternal and Placental Antibody Responses in SARS-CoV-2 Vaccination and Natural Infection During Pregnancy.

Background: As COVID-19 becomes endemic, understanding antibody response and transfer during pregnancy is crucial to inform policy and vaccination schedules. While good immunogenicity has been shown from SARS-CoV-2 vaccines, few data are available demonstrating functional responses in pregnant populations and infants.

Methods: A prospective, multi-site observational study was completed across 14 centers in England from April 23, 2020, to December 21, 2022.

Broadening the Healthy Aging Paradigm: Inclusion of Gestation, Development, and Reproductive Health.

The prevailing focus of lifespan health research has predominantly centered on "healthy aging". This oversight may hinder the understanding of health across the lifespan, as disorders in earlier stages can substantially impact overall health and longevity. Aging, conceptually, begins at gestation.