Publications by authors named "Khalid Pervaiz Akhtar"

Article Synopsis
  • - Mungbean (Vigna radiata) is the second most cultivated legume in Pakistan, experiencing wilting issues in several fields during 2022 and 2023, particularly in the PRI Mung-2018 variety, with symptoms including yellowing leaves and vascular discoloration.
  • - Plants collected from five different locations showed a disease incidence of 5-10%, with roots exhibiting stunted growth and discoloration.
  • - Fungal analysis revealed that the pathogens involved were members of the Fusarium genus, identified through both morphological characteristics and molecular techniques, including study of the TEF1 and RPB1 genes.
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Cotton ( L.) is a significant fiber crop. Being a major contributor to the textile industry requires continuous care and attention.

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Cotton leaf curl disease (CLCuD), caused by cotton leaf curl viruses (CLCuVs), is among the most devastating diseases in cotton. While the widely cultivated cotton species Gossypium hirsutum is generally susceptible, the diploid species G. arboreum is a natural source for resistance against CLCuD.

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Tomato is one of the most consumed vegetables in the world. Diseases are the number one concern in the development of high-yield and disease-resistant tomato hybrids which is the foremost priority of breeders. Present study was conducted (1) to develop DNA-based markers for genetic confirmation of tomato F hybrids, (2) to utilize sequenced characterized amplified region (SCAR) marker linked to the Ph-3 gene for Phytophthora infestans resistance in tomato and (3) to evaluate male and female parental genotypes and their F hybrids against late blight (LB) and cucumber mosaic virus (CMV).

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Cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. The disease symptoms are induced by DNA satellite known as Cotton leaf curl Multan betasatellite (CLCuMuB), dominant betasatellite in cotton but another betasatellite known as Chili leaf curl betasatellite (ChLCB) is also found associated with the disease. Grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cotton (several distinct strains of CLCuMuB are associated with the disease).

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Bt-cotton germplasm, consisting of 75 genotypes was evaluated against cotton leaf curl disease (CLCuD) under high inoculum pressure in the field and using graft inoculation in glasshouse by visual symptom scoring assessments. None of the tested genotype was found disease free under both evaluation tests. Under field conditions in 2011, 3 genotypes were found resistant, 4 moderately resistant, 3 tolerant, 2 moderately susceptible and one susceptible; in 2012, 3 genotypes were tolerant, 7 moderately susceptible, 5 susceptible and 38 highly susceptible; in 2013, one was moderately susceptible and 51 were highly susceptible with varying degree of percent disease index (PDI) and severity index (SI).

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