LC-MS/MS Analysis of Five Neonicotinoid Pesticides in Sheep and Cow Milk Samples Collected in Jordan Valley.

The purpose of the present study was to evaluate the presence of five neonicotinoid pesticides, acetamiprid, imidacloprid, clothianidin, thiacloprid, and thiamethoxam, in sheep and cow milk samples collected from animals bred in the Jordan Valley. In this area, numerous citrus plantations are present, and these insecticides are commonly used to protect plants from pests and diseases. Thirty-seven sheep milk samples and 31 cow milk samples were analysed.

Pharmacokinetic profile and partitioning in red blood cells of romifidine after single intravenous administration in the horse.

The aims of this study were to assess the plasma concentrations of romifidine in horses after intravenous injection, to evaluate the red blood cell (RBC) partitioning of the anaesthetic drug, and to improve knowledge regarding its sedative effect in horses describing the pharmacokinetic model. Eight adult Standardbred horses received a single bolus of romifidine at a dosage of 100 g/kg. Blood samples (5 mL) were collected immediately before romifidine administration (), and at 2, 5, 10, 15, 20, 25, 30, 40, 50, 60, 75, 90, 105, 120, 150 and 180 min after injection.

Levels of perfluorinated compounds in human breast milk in Jordan: the impact of sociodemographic characteristics.

There is scarcity in literature in regards to the exact levels of such compounds in the Middle Eastern region including Jordan. This study was conducted to measure the presence and levels of perfluoroalkyl substances (PFASs) (perfluoroocane sulfonate (PFOS) and perfluorooctanoic acid (PFOA)) in human milk and local fresh cow milk in northern Jordan and also to investigate the relationship between levels of PFASs and some sociodemographic characteristics of breastfeeding mothers and their infants as well as usage of Teflon kitchenware products. Seventy-nine milk samples were collected from breastfeeding women and 25 samples from local fresh cow milk in northern Jordan.

Biliary ochratoxin A as a biomarker of ochratoxin exposure in laying hens: An experimental study after administration of contaminated diets.

To evaluate the levels of ochratoxin (OTA) in kidney, liver and bile of laying hens, forty-five laying hens were enrolled in this study and divided into three equal groups: a control group D₀, and two experimental groups, D₁ fed with 10 µg/kg OTA diet and D₂ fed with 200 µg/kg OTA diet for 6 weeks. Kidneys, livers, and bile from all hens were collected and analyzed by HPLC method for the presence of OTA. Eggs collected 2 days before the start of the experiment and 2 days after its end were also analyzed for the presence of OTA.

Oxidant and antioxidant profile of hyperketonemic ewes affected by pregnancy toxemia.

Background: Negative energy balance during late pregnancy in ewes is an important cause of hyperketonemia. Ketone bodies can generate superoxide radicals and cause oxidative stress and cellular dysfunction, as noted in cows with subclinical ketosis or in diabetic people.

Objective: The aim of this study was to evaluate the role of hyperketonemia in initiating the process of lipid peroxidation.

The relationship between serum biotin and oxidant/antioxidant activities in bovine lameness.

Serum biotin concentrations, erythrocyte superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), reduced glutathione (GSH) and plasma thiobarbituric acid reactive substances (TBARS) were measured in 36 dairy cows, 18 of them were healthy and served as control. In the 18 cows with lameness problems, there were 5 cows with interdigital necrobacillosis, 5 cows with subsolar abscessation, 2 cows with solar ulcers, 2 cows with white line disease, 2 cows with chronic laminitis and 2 cows with septic arthritis. The degree of lameness was estimated to be slight in 3 cows, moderate in 11 cows and severe in 4 cows.

Trace minerals status and antioxidant enzymes activities in calves with dermatophytosis.

The aim of this study was to determine the levels of trace minerals Zn, Cu, and Se, the effect of dermatophytosis on the level of thiobarbituric acid reactive substances (TBARS) as an indicator of lipid peroxidation, the status of enzymatic and nonenzymatic antioxidants, and the relationship between the mentioned trace minerals and antioxidant defense system in calves with dermatophytosis. A total of 21 Holstein calves with clinically established diagnosis of dermatophytosis and an equal number of healthy ones were included in this study. Results showed that 81% of mycotic isolates were Trichophyton verrucosum, while 19% were Trichophyton mentagrophytes.

Risk factors associated with camel brucellosis in Jordan.

During the period between February, 2004 and December, 2006, a cross-sectional study was performed to investigate some epidemiological aspects related to camel brucellosis in Jordan. Four hundred twelve camel sera from 37 herds were randomly collected and analyzed using Rose Bengal plate test and complement fixation test. A structured pre-tested questionnaire was administered to collect information on camel herd health and management.

Seroprevalence of Bartonella henselae and Bartonella quintana infections in children from Central and Northern Jordan.

Objective: To investigate the prevalence of antibodies to Bartonella henselae (B.henselae) and Bartonella quintana (B.quintana) among children from central and northern Jordan.