Publications by authors named "Khaled Fathy Abdel Motelb"
Article Synopsis
- Weed infestations threaten global crop yields, and glyphosate is a common herbicide that targets the EPSPS enzyme, crucial for amino acid synthesis in plants.
- Researchers used CRISPR/Cas9 gene editing to develop herbicide-resistant maize by replacing a part of the ZmEPSPS gene with a modified version, resulting in plants with triple amino acid substitutions that enhance glyphosate tolerance.
- The edited maize showed similar agronomic performance to wild-type plants while containing higher levels of aromatic amino acids, indicating the potential for creating elite maize varieties that can withstand glyphosate without compromising yield.
Article Synopsis
- CRISPR-Cas9 technology has enabled the development of glyphosate-resistant rice, addressing weed threats to crop productivity.
- Researchers achieved this by creating specific amino acid substitutions in rice's EPSPS enzyme using targeted genome editing techniques.
- The modified rice lines showed significantly improved glyphosate resistance, increased aromatic amino acids, and higher grain yields compared to wild-type rice, suggesting a promising approach for sustainable weed management.
Article Synopsis
- * Different isoforms of PAPs are found in various parts of the plant Setaria italica, and their expression increases when the plant is low on phosphorus, helping to improve phosphorus availability.
- * Researchers isolated the SiPAP18 gene and created a protein model based on a similar structure from kidney beans, highlighting five important conserved features and seven metal-binding amino acids that are crucial for phosphorus enhancement under nutrient-poor conditions.
Developing an efficient and reproducible plant transformation protocol relies on callus induction and plant regeneration, which is prerequisite for genetic enhancement of crops, especially rice. The present study has been carried out in order to establish a genotype-independent regeneration and biolistic transformation protocol for rice varieties. Putative transgenic rice lines were confirmed by PCR analysis, DNA sequencing, and Southern analysis.
View Article and Find Full Text PDFGenome editing (GE) technology has emerged as a multifaceted strategy that instantaneously popularised the mechanism to modify the genetic constitution of an organism. The clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated (Cas) protein-based genome editing (CRISPR/Cas) approach has huge potential for efficacious editing of genomes of numerous organisms. This framework has demonstrated to be more economical in contrast to mega-nucleases, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs) for its flexibility, versatility, and potency.
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