Innovative approaches in invertase immobilization: Utilizing green synthesized zinc oxide nanoparticles to improve biochemical properties.

Invertase enzyme can effectively improve the taste, color, and durability of these products. Various methods have been proposed to increase the stability and efficiency of enzymes. One of the most important is enzyme immobilization, which can be implemented on different materials.

Electrochemical aptasensor for Escherichia coli O157:H7 bacteria detection using a nanocomposite of reduced graphene oxide, gold nanoparticles and polyvinyl alcohol.

In recent years, public attention has drawn to food safety due to the constant outbreaks of foodborne diseases; subsequently, to control and prevent this group of diseases, early screening of foodborne pathogens has become significant. In this study, a new aptamer-based electrochemical sensor was proposed to detect Escherichia coli O157:H7 (E. coli), one of the most threatening bacterial pathogens, using nanoparticles-modified glassy carbon electrode.

Survey of Omp19 immunogenicity against Brucella abortus and Brucella melitensis: influence of nanoparticulation versus traditional immunization.

Brucellosis is the most common zoonotic bacterial disease. Prevention of human brucellosis is achieved through pasteurization of dairy products, appropriate sanitation and vaccination of domestic animals against the Brucella species. B.

Structural changes of glucose oxidase upon interaction with gold-coated magnetic nano-particles.

Structural changes of glucose oxidase (GOx), as a typical protein, upon interaction with gold-coated magnetic nano-particles (GMNPs) were studied. GMNPs were characterized by UV-vis, FTIR and SEM. Due to GMNPs interaction, obvious changes in enzyme conformation and its secondary and tertiary structures were verified using intrinsic fluorescence and circular dichroism.

Magnetic nanocomposite of anti-human IgG/COOH-multiwalled carbon nanotubes/Fe₃O₄ as a platform for electrochemical immunoassay.

An electrochemical immunosensing method was developed based on a magnetic nanocomposite. The multiwalled carbon nanotubes (MWCNTs) were treated with nitric acid to produce carboxyl groups at the open ends. Then, Fe₃O₄ nanoparticles were deposited on COOH-MWCNTs by chemical coprecipitation of Fe²⁺ and Fe³⁺ salts in an alkaline solution.

Copper(II) modified carbon paste electrodes based on self-assembled mercapto compounds-gold-nanoparticle.

Three mercapto compounds [2-mercapto-5-(1-methyl-5-nitroimidazole-2-yl)-1,3,4-thiadiazole] (MMNIT), [2-mercapto-5-(5-nitrofuran-2-yl)-1,3,4-thiadiazole] (MNFT) and [2-mercapto-5-(5-nitrothiophen-2-yl)-1,3,4-thidiazole] (MNTT) were used for self-assembled-gold nanoparticle (SAGNP) modified carbon paste electrodes. The electrodes were applied as indicator electrodes for potentiometric determination of Cu(II) ion. The prepared electrodes exhibit a Nernstian slope of 31.