Phenolics and terpenoids change in response to yeast extract and chitosan elicitation in cell suspension culture.

Unlabelled: is an important medicinal plant with antioxidant and anticancer properties attributed to its phytochemicals. To develop a method for bulk production of valuable phytochemicals, cell suspension culture of were grown in liquid B5 medium and then treated in their log growth phase with chitosan (0, 10, 20, and 40 mg L) and yeast extract (0, 400, 800, and 1200 mg L) for 3 days. The levels of hydrogen peroxide (HO), nitric oxide (NO), malondialdehyde (MDA), and the main terpenoids and phenylpropanoids in the cell extracts were determined by HPLC and spectrophotometric techniques.